FastQCFastQC Report
Wed 31 Aug 2022
EGAF00003647223

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003647223
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22
Sequences flagged as poor quality0
Sequence length146
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCGATACACCGAGAACAGGGTGTTCGCCGGCGTGGACAGGTCACAGAA313.636363636363635No Hit
CGCCAAGGCGGCCGACGCGCTGGACCTGTCCTCGGCCGCGCTGAGCAAGC29.090909090909092No Hit
GGCATAATTTGGGTAGCCAAAAAGAGTAATGTAACAAAAGAGAACAAAAG29.090909090909092No Hit
ATACGTTTTTGCCGAGTGTGTTCTGTACGTAAAGCTTTTGATAATAACGT29.090909090909092No Hit
GTGCAATTTTACCGTCATCACGTAAGGTGACCGAGAAAATATTACCCAGA14.545454545454546No Hit
TCTATGGGCTGTCCACTTGGACCACAAATCATCGATCAAATTAAGACAGT14.545454545454546No Hit
ACCTCGGGCTGAAGAGGCCGGTCCGGGGACACAAAAACGACTCTCTGCGG14.545454545454546No Hit
ATAAAAGGTCTCCAAATGGGAAAAGAAGAATTTGAATTATTCCTCTTCAC14.545454545454546No Hit
ATCTCAGCTCACTGCAACCTCCGCCTCCCGGGTTCAGGCGATTCTCCTGC14.545454545454546No Hit
CCCTTGCGTAATTTAAACTGCTCGCTTTGCAGATCGTAGATGGCGTGTCC14.545454545454546No Hit
ACCCATGGTCAAACAATGCCCAAAAATAAAAAATTTTAAATTACAGTATT14.545454545454546No Hit
CGCCAAGGCAGGAGGATCACCTGAGGTTGGGAGTTCAAGACCAGCCTGAC14.545454545454546No Hit
CAAGAAGATCAAGCGCAAGCGCGCATGGCCGAGTTTCGCCCTTTCCAAGT14.545454545454546No Hit
CAGTAACAGAGTCAGGCCACCTGTGAGGACCAGTAGTAACAGGTCCGAAA14.545454545454546No Hit
GTCAAGCTGCTGAAAATCAAACTTCCACGATGGAGAAAGATGTTCGTCCA14.545454545454546No Hit
CTCTGAAGCAATTGTGAATGGGAATTCACTCATGATTTGGCTCTCTGTTT14.545454545454546No Hit
CGCTGCGTGCCTGCGGCGTCGACGAGATCCACGACGGCATCGGCAAGACC14.545454545454546No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)