FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003647225

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003647225
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44
Sequences flagged as poor quality0
Sequence length146
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGAAGAAGCAGCCCATTCAGGAAACTTTAATAAAATGTGGGTAAAACAA511.363636363636363No Hit
CGTCGAGCACCGACTCGAACTGGCCCGCCTTCGATGACGCCACCTGGTCC49.090909090909092No Hit
GCGGCCCCGCCGTGATCGACACCGCGCACTGGGCGAGCGAATTCCCGTGG36.8181818181818175No Hit
ATGTGCTTCCGATGTACTTCTGCATTAAATTCCTTGCTTTCAGAATCATA36.8181818181818175No Hit
AGGCATTGCTCTCGAACAGCACGAGCCGCCCGGGCTTCCACGCGCGGATC24.545454545454546No Hit
ACCTTGGCGTACCCCATCGCCTGGGTGGCGGCCGCGACGATGTCACCATC24.545454545454546No Hit
GACTTAGTCCACAGTCCACAACTTGCACGCTGTCATTATAGTCACCCTTG24.545454545454546No Hit
ATACTAATGTTGGGTGTAAATGGCCTAAATGCTCCATTTAAAAGATACAG24.545454545454546No Hit
CCTGATCAAGAAACAGAAAATTACAAAGAGAAGCTACTCACATGTACCCA24.545454545454546No Hit
GGATAACTGGCTTGTGGCGGCCAAGCGTTCATAGCGACGTCGCTTTTTGA12.272727272727273No Hit
AATCTTTTATGATGAAACAGAACAGTTTTCTGTTTAAATTATACACAAGT12.272727272727273No Hit
GCCACTTTAGTGACAGGAGCTTTAACACTTCTGAATCCTGAGGGCTTGTT12.272727272727273No Hit
CTCAATAAATAGTAGCTATTACACTGATGATGTATGGGCAATCCTGAAGT12.272727272727273No Hit
GATATATTAAGATTCACTGGGTGCGGGCCAGGCACGGTGGCTCAGGCCTT12.272727272727273No Hit
AAAGAGTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGT12.272727272727273No Hit
GCGTTCACTAAAGGGACTGGTAGCAATGGGAGAACATTAAATACAAATCA12.272727272727273No Hit
TCACTGAGCAGCTCTTGACCATGAAAGGTAAGTGAGAGGTTGATGATGTG12.272727272727273No Hit
TCCTTACAGAATCCTATAAAAACACACACAGGGAATTTTTATGATTTTTT12.272727272727273No Hit
GTTTGAGGGCATTTATCAGGCTTTTAACTCTAGGTACTCTTTCCCGCAGT12.272727272727273No Hit
CATGGGAGAGGAGTGACTTTGACTGCTGGGGAATTTCCTCCTAAGAACCT12.272727272727273No Hit
ATATTAAAGACTACTGTGTGGTGAATAGACTCATGACCAGTTAGGATGCT12.272727272727273No Hit
TCCCATTTCCGTGTCACCTTCTTTCTCACCACTGGGGCCCCTTGTCAGGA12.272727272727273No Hit
GCTTAAGACCAGGTCCTCTGCTCCGGGTCTCACGAGACTACAAGGAAGGT12.272727272727273No Hit
ATTCCATTCCATTCCATTCCATTCCATTCCAATCGAGTTGATTCCATTCC12.272727272727273No Hit
CATCAGGATATGTAAAGGAAGAGGATTTGTACCTTGTGGTTCTAGGGAAT12.272727272727273No Hit
GCTCTGTCGCCCAGGCTGGAGTGCAGTGGCACCATCTCGGCTTACTGCAA12.272727272727273No Hit
GGTAAGGGTTCCGCTCAAAGCAGGCTCGCATGGCCTCGCTTTGGCTCGGG12.272727272727273No Hit
TGGCCTCAAGTGATCCGCCTGCCTCAGCCTCCCAAAGTGCTGGAATTACA12.272727272727273No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)