FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003647226

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003647226
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44
Sequences flagged as poor quality0
Sequence length146
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTATTACCTAGGCCAAGTATTATATGAGCTATTAGTTTATAGAATACA511.363636363636363No Hit
CGTCGGTGGTCGAGGACCAGGTGGCGTCATCGAAGGCGGGCCAGTTCGAG49.090909090909092No Hit
GTCCACGGGAATTCGCTCGCCCAGTGCGCGGTGTCGATCACGGCGGGGCC36.8181818181818175No Hit
GATGAATACAATGTGGAAAGCATTGATGGTCAGCCAGGTGCCTTCACCTG36.8181818181818175No Hit
ACCTGCATGTACCGGATATGGTCTGCGATGGTTGGGCCGAGTTACACCGC24.545454545454546No Hit
GTTGACAGCATTAAATTCCTACATCAAAAAAGTCTGGAAGAGCACAAATA24.545454545454546No Hit
ATATAGCAAAATGATAGAAAATATTGTTAGTAGGTACTAGAAATGGAAGT24.545454545454546No Hit
ACAAGGGCCTGAACGGCATCGCCATCGACGGCGGGCGCGATGTCTACGTG24.545454545454546No Hit
CTCCCAAGTAGCTGGGACTACAGGTGCATGCCACCATGCTCGGCTAATTT12.272727272727273No Hit
GGCACCTGTGTTATGAAATGCCTATGCAGAAGGCATCATGGCAGAGATAT12.272727272727273No Hit
TCATTTAGAGGACATTCACTGAACAATGCCAGTGATACAAGTGCCATGGA12.272727272727273No Hit
TCATTTAGAGGACATTCACTGAACAATGCCAGGGATACAAGTGCCATGGA12.272727272727273No Hit
ATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATC12.272727272727273No Hit
CACAAAAAGCACCATCCATAAAAGGAAGTATAGATAAACTGTACTTTATC12.272727272727273No Hit
GTATATAGTATACATACTTGATTTTGGCCACAACAAACAGATCATTGAAT12.272727272727273No Hit
ATTTTGAGTGTTTCTTGCCTTTTCCATTTATAATGAGGAAAAGTGAACAC12.272727272727273No Hit
GGGACAGGCGGGGGACCGGCTATCCGAGGCCAACCGAGGCTCCGCGGCGC12.272727272727273No Hit
GTACACAGCTTACATAAAGAAATGTGCATGCCATTTTTTTGTGAAAAAAA12.272727272727273No Hit
CACCTGAAGTAGGACAAGATCGGCGCCCCCACCATCATTCTCCATGTGAC12.272727272727273No Hit
GAACTCAAACACGTTAAGGCAAGTGAGGAAGGTAAGTTTGTTTGTTCTCT12.272727272727273No Hit
GTATTTTTTTCTCTTTGTACCAGCTGAGTTGGCCTTCTTTACATCAGAAA12.272727272727273No Hit
AAGGAGGACGTCAGAATTAAATGCTTGATGGAATTGACATAACACATCCA12.272727272727273No Hit
GCCGCTTCCTGGCTGTTCGGGCCGGCCTCCTGGCTGCACCTGCCGCAGTG12.272727272727273No Hit
GGCCAAGAAGAAGGCAGCCAAAAAGAAGGAGGCTGCCAAAGCTCGACAGC12.272727272727273No Hit
GAATTAACCCGAATAGAATGGAATGGAATGGAGTGGAACGGAACGGAATG12.272727272727273No Hit
GCTTTGAAATCACGTTGCCTGCTATTCCTGCCGCTGGGTCAAACATCATC12.272727272727273No Hit
ATGCCCTGGAGTCAAAGACTGCTGCCAGCGGGGTGGAGGCGAACAGCAGA12.272727272727273No Hit
GTAGTGCAGATGGTGAAGGAAGAGAAGGGGATAAAGCAGAGAAGTCCAAT12.272727272727273No Hit
GTACAGAATATGAATCATAAGTTTGGGTGGGCCAGGCACGGTGGCTCACT12.272727272727273No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)