FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003647231

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003647231
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37
Sequences flagged as poor quality0
Sequence length146
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGGAAGCCGGCTATTACACGGCCGACACGGAGAACGACCGGGTGACGC38.108108108108109No Hit
GCCACGACCATTGCAAGTTCTTCAACAAAGTCAACGGCAGTCAGGGTTGG25.405405405405405No Hit
GGATATACTACAGCGATGGCTATTGAGGAGTATCCTGAGGCATGGGGNTC25.405405405405405No Hit
GTCCATGGCATGCTGTACTGGCAATGCCGATGCTATCACCTGAGCTTGTG25.405405405405405No Hit
GAGCTAGATGGGGAATAAAGAGATTTCAAAGCCAACAAGAAAAATGGTCT25.405405405405405No Hit
ACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCAAC25.405405405405405No Hit
CAGTGAGAGTGAGTAGTAGAATGTTTAGTGAGCCTAGGGTGTTGTGAGTG25.405405405405405No Hit
GCATCGATGTTGCCGGCTGCAGCACCGGAGAGTTGGTCTTCGGGCAAGAA12.7027027027027026No Hit
AGCTTTGTGTGAGTACACTCTATGATGTTTGCACTATGACAAAATTGCTT12.7027027027027026No Hit
GGCTGTGTCTAATGCCATAAGAAAAGTAAATCAGGAAGCCAATTGCCATC12.7027027027027026No Hit
GTTTCTGCCGAGAGATCTGCTGTTAGTCTGATGGGCTTCCCTTTGTGGGT12.7027027027027026No Hit
ATTCATGTTTCTAGCGTTATTGAATGGATTAGCGCTATTTGGTTGATTTG12.7027027027027026No Hit
CAGTATGCCGAATCCACTCCCGCAACAACGAGAAGAGTCACGCCCTGTGA12.7027027027027026No Hit
CTCTAGTAGAATTAGAGCAGAATTCCATCCAGGAGGAACATTTTTCTTTT12.7027027027027026No Hit
GAGAAGGACTCTGTCTCCAAAAAAAAAAAAAAAAAAGTACTCTGCGTTGA12.7027027027027026No Hit
GTGCAGGGACGGCTGAGCCTGATTCCCTGGGTGTTCTTCGCGGTGAGCTC12.7027027027027026No Hit
GGGGCCTCCCACTTATTCTACACCTCTCATGTCTCTTCACCGTGCCAGAC12.7027027027027026No Hit
GATAGTAAGCAGCTCGCTGTAACTGTTCAGTGGCTCTTTCGACGTGCACC12.7027027027027026No Hit
GAATTTACATCAAGTTTTAGCTACATTGTTGAAATACAAATATGCAGATT12.7027027027027026No Hit
CACAAATTTTCTTATGAAAATTTCACAATTTTCTACCTAACACACTTCTG12.7027027027027026No Hit
AATTTGAGCTATTGCTGCTTCACTCGCCGTTACTAGAGCAATCCCTGTTG12.7027027027027026No Hit
CCCATGAGAAGGGCGCTGTTATTCTTATCTTTGTTACAATGTTTGTGATG12.7027027027027026No Hit
TTGTTATATCTCCTTCTCACCATTGGCTATGAAAGTATTCTCCAAATTCC12.7027027027027026No Hit
GTTGTAGACTGCACAATTAAAACAGATCCAGTCACTGGGAGATCAAGAGG12.7027027027027026No Hit
GGTCTTTACAATTTGGCATGTTTTTGCAGTGGCTGGTACCAGTTGTTCCT12.7027027027027026No Hit
CTTACGATGAGCGCCTCGATGTACTCTTGAACCAGGTCTTGGTCATCCTC12.7027027027027026No Hit
AGGTAGCGGCGGCCTTCGTAGGTGAAGGAGAACGCGGTGTCGCTGCCGCC12.7027027027027026No Hit
GCTCGTCCTCGGGCTCGTCGCGCCGGGGGACCCGCTGCCGGACGCCCCGA12.7027027027027026No Hit
GGTAAAGAATTCATGACAAAAATCTCAAAAGCACAGGCAACAAAAACCAA12.7027027027027026No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)