FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003647232

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003647232
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37
Sequences flagged as poor quality0
Sequence length146
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCCATCGAAGCTGCTCGGCAGCCCTTCGTCCACGCGGGTCCAGCGGTCG38.108108108108109No Hit
CATTAATCAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATACCGTCG25.405405405405405No Hit
GATTATCATATCACAAGCTCAGGTGATAGCATCGGCATTGCCAGTACAGC25.405405405405405No Hit
TCTCTATACTAATCTCCCTACAAATCTCCTTAATTATAACATTCACAGCC25.405405405405405No Hit
CCAGTAACTACTACTAATCAACGCCCATAATCATACAAAGCCCCCGCACC25.405405405405405No Hit
CCCAACAGGAAGCTATTAGGGGAGAGACCTTTTCCCTGTGCCCTCCCTCC25.405405405405405No Hit
GTACGAATTGTAGGCCCAACCCTGACTGCCGTTGACTTTGTTGAAGAACT25.405405405405405No Hit
TCTCAGAAATCACCACTAAACAGCTTATCCATTTAACAAAACACCACCTA12.7027027027027026No Hit
GGGTTCGACCTCGATGCCGTTCTTCGCCACAATCTCCCGGACCAGCACCC12.7027027027027026No Hit
GTCATGTATTGTTTAAGGGCAGGGATAAGCTCTGAGAAATGGGTCTTTAA12.7027027027027026No Hit
GCAGAAACCCTACAAGCCACAAGAGAGTGGGGACCAATATTGAACATTTT12.7027027027027026No Hit
GCCTCCAAACCCTGAACTCTCTTGCCCACAGGAAAGGTGGTGAAAACTTT12.7027027027027026No Hit
ACCCGACGCAGCGCGGCTGGTTCCTGCAAGCACGCAAGGCGGGCGGGCCG12.7027027027027026No Hit
CTTTAAAAGAAATATGGCGCAGGTGAACATTTGCTTGGAGAGGGGAATCC12.7027027027027026No Hit
GTCGTCGGGATCGAGCGTGCCGTCGCGCAGCCCGGCGGCGAGCTCGGCGT12.7027027027027026No Hit
GAAATGTGGGACTATGTGAAAAGACCAAATCTACGTCTGATTGGTGTACC12.7027027027027026No Hit
ACCTTTTTTGGGAGGTTCTTTCCCTTTTAAAGCTTTTGGCCCTTTTGGGA12.7027027027027026No Hit
ATCTTGGTGAACATTTACTTGATGGTCCAGTTAAGTGCAGACACTTGGGT12.7027027027027026No Hit
CATTTGTACATCTTCTTTTGAAAAATGTCTATTCATGTCCTTTGCCCACT12.7027027027027026No Hit
TAGTATGACGAGTTAACAAGTTGGTGACCTGCACAAAGCGAGACACAGCT12.7027027027027026No Hit
CTGCCGTGATGGACACCAGCTCTTTTAGCTGCGGGTTTGGGGAGGCCGGG12.7027027027027026No Hit
TCCCTCGACAGCTCACACCCTGAATCGCCGGAATCGAAGGGGATCACCTT12.7027027027027026No Hit
CTTTTTATCATGTTTGATGTCCTAACAATTATTGTTCGTTTATAGTTATC12.7027027027027026No Hit
GCTTGCAGTGTTACCATCCATTCTAAAGATTCAGCATTATCAAAAAAGTG12.7027027027027026No Hit
CCCTATTAGTGGGTGAACAATCCAACGCTTGGCGGAATCAGCGGGGAAAG12.7027027027027026No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTGGAGACAGAGTCCTT12.7027027027027026No Hit
TCTCGAAGCGCTGCCCGTTCAGGAAACCGCCGACGCCGCCGCAGAACTGG12.7027027027027026No Hit
GCTTGGTATTGGGCAACGCGGTCCGCCGCGTGCCTCAAATCGTCCGGCTG12.7027027027027026No Hit
GTGATGGCCACCTCATCCTCCTGGCTCTGTTGTAACTGTTGCAGAGCCAG12.7027027027027026No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)