FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003647235

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003647235
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences123
Sequences flagged as poor quality0
Sequence length146
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGAATATAAGCAACTTCAGCGGAAATCGGAAGATGAGATCGAAAAGGA43.2520325203252036No Hit
TTGGTCGACAAGTGATGCCATGGCACTTGCCACAATTCCGAGTTCCTGCA43.2520325203252036No Hit
CTCAGCAGGAGGATCGATTTCCCGCCGCTTTGCCAAAAAGGCTGTTGAAA32.4390243902439024No Hit
CTTTAGACATCTGTTCTTTTGCAGTAGTATTATTGTGGAAAATCTCCGAC32.4390243902439024No Hit
GCGACAAACATTCCGCCGCATCAGCTCGGCGAAGTGATTGACGGAGTCCT32.4390243902439024No Hit
GTACATAAACAAGAAATTGATTCTGCTCCTCGACCCGATAAAGAAATTAG21.6260162601626018No Hit
TTTCTTCCGCTGTCGTAACGTAAACGACTTCACAACCCGGCGGCGGGGCA21.6260162601626018No Hit
ATCTTCTTTGGCAGCCTGAATCATGACGCCCGCACCAAAGGCCAGCCCAA21.6260162601626018No Hit
CCGCATGATTCAATCCTCACCGGCCCTCAAAGAAGGTGCAAAGCTTGCTG21.6260162601626018No Hit
CGTATGATGTGTTGCTGCAAATTAAAGAAAAAGGGCGTTTGCCTGTTGTT21.6260162601626018No Hit
GGGCTGAAAAAAGGAGAAATCGTTCTTTCGCACGAAATGCTCAGCGAACT21.6260162601626018No Hit
GTCCTGGATCAGAGCCAAAGGATTTTACCACTCATAGAACAGCAAGCAGC21.6260162601626018No Hit
GTTCAAATTCATCCGGTGTTTGTTTTCCCATGTTGGTTTCACGAATGTCC21.6260162601626018No Hit
TCTTTTGCTCGGTCAGCGCTTCAGTCTGCGCAATTTCGTTTTCGTGGTGA21.6260162601626018No Hit
TTGTATATCTTCCATAGAGAAATATCTGCAGCCAGGTACAGTGGCTCATG21.6260162601626018No Hit
TATTGGAAATGCTCGACCGGCAGTCGTTTTCGACACGGTGCGCCGTTATT21.6260162601626018No Hit
TAATTGCATGCCTCCGGCGTCTCCTTTTTCTTTTTGTTCCCCATTATATC21.6260162601626018No Hit
GTATAAAAACTTCGTCAAAGCGAAAGCCCGCTCTTATTTCCTCATCGGGG21.6260162601626018No Hit
GATCTATGGAGCGCTTTTAGATGCAAAAGCCGCTGAGCATGCAGCGCGCA21.6260162601626018No Hit
GCGTAGCCGGCCTGAGAGGGTGAACGGCCACACTGGGACTGAGACACGGC21.6260162601626018No Hit
ACGGGATGATCGTCTTAAGTTCCGCCAAAATTAAGCGACCGTTGCGCGAA21.6260162601626018No Hit
TTACAATATTTATGAAGCCACGAAAATGGCGATGACACGGGCCGTAAAAT21.6260162601626018No Hit
GTTCCATCAATAATTCTCCTTTTCTTATACAACTTTTTTCGCAAGAAGAC10.8130081300813009No Hit
GAATTGTCCGAACATCAACGAATTTCCCGTGAATCGCCGCCGCAGCCGCC10.8130081300813009No Hit
CTTTCGCAAAGGCTTCTCTAACTTGTTTCGTGACGACTTCCTCCGTCTTT10.8130081300813009No Hit
GCCAATTCCCGCGCCGCTGCTTTTGTCAGCCCGATGACGCCGGCTTTTGC10.8130081300813009No Hit
CAGACAGGAAGGCGAGGATCAACAAGGCGGCCTACCGTTTGGCGGCGGCA10.8130081300813009No Hit
ACGCCGAGCCGCATCACTTCGGCTCCGATCGACAACAATCCGGCAACAAG10.8130081300813009No Hit
GCAGTGGAACGAGAAATTGATTCCGTATTGGCGGGAGGCTGGGAAATTTG10.8130081300813009No Hit
AGCCAGCGCAGCAGAAATGCCAATTTGATAGGCGTCGCCTCTTGCGTAAT10.8130081300813009No Hit
CATTACGTCTGCCCATCCTGTTGCTATTCCCGATTTTTTAATGACGGATC10.8130081300813009No Hit
GGACGGGGCCCTCGGGCGCCGGTCGTCGAGGGGGCGGCGGGCCTCACTCC10.8130081300813009No Hit
CGATAAACATCGTGTGACGGGAAATCCTCGGATTTCAGAAACGATTGAAA10.8130081300813009No Hit
CGGCATACTAATGACACTCGCGGCAATTCCTTCTTTTCTTAATTCTTTTT10.8130081300813009No Hit
TATCAGTACTGCATGTTACATATTCTTCTTTAAAATTTTGTAATAAACAT10.8130081300813009No Hit
AGCCGGGATTAGGGTCGACGGCTGTCCAACCTATCACGGGCGGAAGCGCT10.8130081300813009No Hit
GGAAAGAAATTTTGCAAGTGTATGATTCACCAGTCCGTCCGAATGTTGAA10.8130081300813009No Hit
AGAAAATATAATCCAGAGAAACATGAAAAAGCAATTACAATCCCGACTTC10.8130081300813009No Hit
CTTTCGGCACTTGGTCATTCATCAGAAAACGTGAATGATATCACACTTGC10.8130081300813009No Hit
ACAACATTCGTTGACGTTTTCATCGGACAACCCGTCAGGCAAGCTAAGAA10.8130081300813009No Hit
CTGCAACCCGATCTCGGGACGGCGCTCGTGCTGACTGCAATTACGTTAAG10.8130081300813009No Hit
AATACATGCTCTTGACCAGATGCAAGCGCCTCCGGCATAAAACCGATCTC10.8130081300813009No Hit
ATGTAAAGTCGTTCTTGTCGGGCGTTTGATGTCTGTTACAGTCCGCCATT10.8130081300813009No Hit
TGCCTAAGAAATCGGACAATCTTTTACATCGTGCGCTGAGCGCGGCTGTT10.8130081300813009No Hit
TTCCTAACGTCGGTAAATCGACGCTTATTAATCGTCTGGCAGGAAGAAAG10.8130081300813009No Hit
TCGCTGGAGGACTGGTACTCGGTGGTGGTGGTCAGCCTGCCCATGCCAAT10.8130081300813009No Hit
GTATGGATCGCCTTTTCGCCCCATTGCTGAAACGACAACGACGACTTTAT10.8130081300813009No Hit
TATCTGATATGACATGTCCAAGCGCCCCATATTTCATCGTTTTTGGATGA10.8130081300813009No Hit
CCCGCAACGGTTTTAGACAGATTGAGGTGACCGGAAAGTGAAATGGAATG10.8130081300813009No Hit
GCCGTGGCGCTATCAATAATTTCAAAACCTTCCTCGAGGAAAATTTCAAC10.8130081300813009No Hit
TATCTTGACAGAGAAAAAAAGCAAGAACAATATTGACAGCACGCATTTTA10.8130081300813009No Hit
CTTCTAGACATTCCCATTGACAAGTACGTTGCCGTCGGTTTTAAAGGATT10.8130081300813009No Hit
CTCCACCATTTTCGGATCAATCAGCAACAGCTTTACATCTTCTGGTTTTC10.8130081300813009No Hit
GGCAAGTATACGTTCTCTTCTTCAAGGACAATTTTTCCTTCCTCGTCAAG10.8130081300813009No Hit
ATTATGGTGTTGCCTTTCCAGAAGGTTCCGAACTTGTGGATGATGTAAAT10.8130081300813009No Hit
GTGAGAGGAGCTTCGACATGAATGGGTCGCCTTCTCTTGAAGGAATTCAA10.8130081300813009No Hit
CTGATGACGTCCGCGCTGATGTCATCATTTTGACGCACGGACATAATGAT10.8130081300813009No Hit
GTTTATGTATGTTAGAAAACCTTACAAGGAATTTTGTAAAACAGCACGGA10.8130081300813009No Hit
CCATTTGTGAATCCGGGTATGAAATTTTGACGAAAGCCTAAATGAAGGTG10.8130081300813009No Hit
AAGGTGCCGAACGAGCACATCGACTTTTGCTGCGGCTCGGGGAGGACCGT10.8130081300813009No Hit
CGAGGGGACAATGCGCGGGAAAATCAGTCCGATTTATGAAAAGACGGAAA10.8130081300813009No Hit
CATCGGCACAGTGGGGTCGGGAAAAATCTGTTGCAGCGGCCGTGGGGTTT10.8130081300813009No Hit
ATGCAGAACTTTCTCTCGCCTTCTTGTGCGTTCTGTATCCGGTTTTATTT10.8130081300813009No Hit
GTATATAAGGGAACCATGCTTTTGTGAGAGAAATCGACTGTGGTGGTTTC10.8130081300813009No Hit
GATCTGAACAAGCCTTTTTTCTTGGGCGCGTTTGAGAATCGACTCGTTGA10.8130081300813009No Hit
CTTCGAAGTCCATGACACAAAAGACCCCTTACGATGGCAAAAGTATTGTA10.8130081300813009No Hit
AAGTAAAGGTTCCGTTCATGCGAATGGCCGTTTCCATAAAACGAACAACG10.8130081300813009No Hit
GGAAAGAACTTCCCGCACGCCTCCATTTTCCTCGCCGCCGACGGCCACGA10.8130081300813009No Hit
AAATGGAAGGGTTTGTCATTTCAACTTCATACAGCAAAAAACCCTCAATC10.8130081300813009No Hit
GGGGATGCATACATCGCACAGTCCGGCAACGAGAAAATCTTTTTAAAACG10.8130081300813009No Hit
CGCATCAGCCAGTCGGTTGCTATCTTTGTCCTGAACTACGGCAATCACCA10.8130081300813009No Hit
GGGCGCTCGGTACGGCGCCCTGACCTGACATTTGCCACGATGGATCATAA10.8130081300813009No Hit
AAACTTCCCTTACCAAAAATGAGGTTTGTCTAAAATCATCGAAAACAGAC10.8130081300813009No Hit
GGCGTAGTCCATCGTGACCACGCGGTAGCCTTCCATGGCGGCCTGCAGCG10.8130081300813009No Hit
AAGTAAACCAGTTCATTTCAGAAAGAAGGCAGAAGCATTTTTTAAAAACT10.8130081300813009No Hit
CATTAACGTTGCTGACGAACTTTACGGATACAACCGGGAAAGGGCATTAA10.8130081300813009No Hit
GTGGAGAATGCTGTTTTTTCAAGTCCGTGGACCAAACAGGCATTTTATCA10.8130081300813009No Hit
TCTCCATACAGTACGACAGTCGTTCCTTCTCTATTTGCCAGCGCGCTTTC10.8130081300813009No Hit
CTATTGTATAGGCCTTAAAATTGCGGGTATCATAAAGTCTCTATGGTACC10.8130081300813009No Hit
GTACGTGCGGTTGGCGAACGATGGGGAACAGTTGACGTGCTAGTGACAAA10.8130081300813009No Hit
GGGCTGGGAAAGAAATTCTCCGTGATCTTTACGAGCGCGGGGCCGAGGAA10.8130081300813009No Hit
ATTAAAACCTGGGCCCGAATCGGCAGCTGAGGGGAATCCGGGTTTACTTT10.8130081300813009No Hit
CTCTCCAGGTCTCCAGCTGCACGACACTCATACTAGAAAAATGCCAGCTC10.8130081300813009No Hit
GTCCTCCAGTCTGAATCGTTTGCGAGAAAAAGCCGGATTGGACCTCTTGC10.8130081300813009No Hit
GTGAATCAATCTCCTATGATGAGACTGTCCACGAGTTGGTCACCCGTTTT10.8130081300813009No Hit
GTAGACGGGAACGTCATGCGCGTACTTAGTCGTCTGTTTAAGATTGAAGA10.8130081300813009No Hit
GCCTGCATCCTGACAGCCAAAATTCACCCCGTCGAGGACTCGTTGCGGCA10.8130081300813009No Hit
CAATACATCCGGATGCTGCCGTGCGCGGTCTCGGCATCGTGGGCCTCGAA10.8130081300813009No Hit
GGACTGACGAATGTGAAAAAAACGGTTCGCGATTTAAACGGTTCCATCGT10.8130081300813009No Hit
GTACAGGCAGACGACAAGCGGCAATTCTAGGTAGATGATCGTGTCTGCCT10.8130081300813009No Hit
AAGCAAACCGTATCCAACCTTACGCAACTTGTATCCGAAGACGTGCAGGC10.8130081300813009No Hit
CTGTAGAATGGAATACGGTTCGTACATACGACGAAATTTTGTATGAAAAG10.8130081300813009No Hit
GTTCTGGCGCTTGCTGAACGAAGCGTCTGGATTACGCTTCTCATTTCCGC10.8130081300813009No Hit
ACAAATTGTTCAATGATTTCAAGCTGATGATCATCATTATGCTCACCATA10.8130081300813009No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)