FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003647245

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003647245
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33
Sequences flagged as poor quality0
Sequence length146
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTGCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAATGAAGCGCGGG39.090909090909092No Hit
CGAGTGCTCAGCTCCTGGACAGGATGGTGAGTCGATGCAGATTGGCAACC39.090909090909092No Hit
ACATTAATGTTATGTAAATAGTTGTTTTACAGTATTGTTTAGAAAATAAT26.0606060606060606No Hit
GGGTTGCCGCTCGCCCAGCCCAGCAGATCATGAAAACGCCGATTGGTTTC26.0606060606060606No Hit
ATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA13.0303030303030303Clontech Universal Primer Mix Long (95% over 21bp)
AGAATTACCACAGTTATCCAAGTAGGAGAGGGAGCCTGAGAAACGGCTAC13.0303030303030303No Hit
CCACCAGGAGTGGAGCCTGCGGCTTAATTTGACCCAACACGGGAAACCTC13.0303030303030303No Hit
GAATCAATGGGAGACGAAGGTGTACGTCATTAATCCCAAACAGATCAAAA13.0303030303030303No Hit
ACCACAACCGGCGACAAGGCAGGGACCTCAACGAGGTCACGCTTTCGGAT13.0303030303030303No Hit
GGCCATGATGATCAGTCCCCACGACTTGGCCGTCAGGCCCAGCCAGGTGA13.0303030303030303No Hit
GGTTCTAGTGGTTCATCTGGCATCTTGGCATAACGGAACTCAAATACATC13.0303030303030303No Hit
GTGAACCTGCAGCGTCATGTGGAGAACATGCGGCGGTTCGGGGTTCCTGT13.0303030303030303No Hit
GCGGTGTGCTGTCCATACATGCAGTGGCCTGCCAGCACTTGGGAGAATGC13.0303030303030303No Hit
CACATTCCATACTGTGGCTGACATGATGTATTTCTGCCAGATGCTGGCAG13.0303030303030303No Hit
GCGTTGGATTGTTCACCCACTAATAGGGAACGTGAGCTGGGTTTAGACCG13.0303030303030303No Hit
GTACACCTGTCAAACGGTAACGCAGGTGTCCTAAGGCGAGCTCAGGGAGG13.0303030303030303No Hit
GAGCTGCTCTGCTACGTACGAAACCCCGACCCAGAAGCAGGTCGTCTACG13.0303030303030303No Hit
GATGGAGACGCACCTGGGAGAGGACAGCGCCCCCTCTGTATTTTATGCCG13.0303030303030303No Hit
AGGCAGGAGGCATGCTGCCGTGACCGGTCTGGCTCGTCGTGGCGGACGCT13.0303030303030303No Hit
CTCCCAGACCCAGAGATTTTCCGACAGCGATTCAGGCAGTTTGGATACCA13.0303030303030303No Hit
CATATATATTTAGGATAGTTAGCTCTTCCTGTTGAATTGATCCCTTTACC13.0303030303030303No Hit
GTAATGAGGCGTGCGCCGCCAATATGCACTGTACATTCCACAAGCATTGC13.0303030303030303No Hit
GCCCAGGAGACCTCGGACGCCGCGTTCCTGCGCCACATGCTTCAGGTGGA13.0303030303030303No Hit
CCATCATCTGTTGTTTCCTCACTTTTTAATGATAGCCATTCTAACTGGTG13.0303030303030303No Hit
GACTTTGAAGCTGTCACACAAACAATTACATTTCACTGTGATTGAAGTAA13.0303030303030303No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA13.0303030303030303No Hit
GCCGGGTTCGATCGTCGCGAGACGGACAGCCAGGCCACGGCCGTCTGGGG13.0303030303030303No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)