FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003647247

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003647247
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44
Sequences flagged as poor quality0
Sequence length146
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTATTGCCACAACTAACCTCCTCGGACTCCTGCCTCACTCATTTACA36.8181818181818175No Hit
GCCCGTTCCCTTGGCTGTGGTTTCGCTGGATAGTAGGTAGGGACAGTGGG24.545454545454546No Hit
AGATTAGGTTGGCTGTTAAATGGAAACCAACCTGGTCCTTGCATGTTGTC24.545454545454546No Hit
GTTCCACTCCTGTCAGTGTCAAAACTGATAAAGTGTTGTCTCCAGCCATT24.545454545454546No Hit
GGAGGATAAAATAATCTTAAATTTTGACACCTCGTCTAGGTCTAGAAGTC24.545454545454546No Hit
CGTTTACGCTGTAGGATCCTCATTCCCACTGGCTTTGAACATTTTGGGGA24.545454545454546No Hit
GATGTGTATCGTGAACGTTTGGGGCCTGTGCTCGCCTAGTCCTCATCTTT24.545454545454546No Hit
GGCCGTAGCCCGGCCTCTAGCATCGACCGGTGGTACCCTTCCAGGCGCTT12.272727272727273No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA12.272727272727273No Hit
CCGTGATGTACCTCAAGCGGTGTTGCCCAACGGAATGAATACCAGAGAGA12.272727272727273No Hit
GTGGTGCGCGCGATGTTGTTCTCGGCCTGCTGCGTCGTCAGCGACGAACC12.272727272727273No Hit
GGGTTTTACCACATGGTCAGCTCCAAGAAAGAAGCACTCAGGAAGCATAG12.272727272727273No Hit
ATCCAACTGTCTTAGAATTTTTTCTCTGTAAATTTATACTTGACTTTGTT12.272727272727273No Hit
CGTTCAGGCCGGAGGCGTGGTCGCGGAGCTCATAGAGGATCTCGTCCATC12.272727272727273No Hit
CATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGACCCGGGGA12.272727272727273No Hit
CTATTACTTTGTTAATGTTGCTGGACCTTGAACCACATTTTGAATAGCAC12.272727272727273No Hit
GATTGACCAAGATGCTCGGGATCTCTATGACGCTGGAGTGAAGAGGAAAG12.272727272727273No Hit
AGATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGC12.272727272727273No Hit
TCGTTGTTCTCGGCTGCCTCTCCGGCGCACTCGGCGGGCTGCTGAGATCC12.272727272727273No Hit
GTGTTATGATGATGGGAAGACATACCACGTAGGAGAACAGTGGCAGAAGG12.272727272727273No Hit
CCCCCACACCATCTTAGGCGCCGTGTCCTTGGCGGGAGCCTTCCTGCTCG12.272727272727273No Hit
AGTGGAATCAACTCATTCAGGGGGTGAAAGTTTTCATCCATGGAGACAAC12.272727272727273No Hit
CAAAAAAATGGGGGCGTAACATAAGGAAAACAAGCAGAGCAGTTACCACA12.272727272727273No Hit
CACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCA12.272727272727273No Hit
GCATAAGAGTCCTATTTGCCCCAGTTAATTCAACTTTTGTCTGCCTGTTT12.272727272727273No Hit
GACTTTAGAGTGACTAATGCTTATTTTCTCAATGTAAGCACATTCTGCAA12.272727272727273No Hit
GTGATTTCTGCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAATGAAG12.272727272727273No Hit
GATGTTAGCAGAAGTATGACATGGGACTGCTAGGACAGCACCTTGAAAGG12.272727272727273No Hit
ATACAATATTAGCATGGATCGTCTGTTCTAATATGCTGCAAGCAGGTAAA12.272727272727273No Hit
TGGGTGAGATGTTCGCCAGCCTCACCGACAACCTCACCACCCTGGTCCGC12.272727272727273No Hit
GCCATAGGTGTGCTGGCCACCATCTTTCTGGCTTTGTTTGCAGCCTTGGT12.272727272727273No Hit
CTAAGAAGTTGGGGGACGCCGACCGCTCGGGGGTCGCGTAACTAGTTAGC12.272727272727273No Hit
GTTCCACGCCTGTCAGTGTCAAATCTGATAAAGTGTTGTCTCCAGCCATT12.272727272727273No Hit
CGGCCACGGAGATCCATTTCATGCCCTGCTCCAGCTCTGCCGATCCCGCG12.272727272727273No Hit
GTGACAGCCCCCACAGCCCAGACATGAGCTACGAGATCATGAGCCAGGAC12.272727272727273No Hit
GGTTACCGCAGCTAGGAATAATGGAATCGGACCGCGGTTCTATTTTGTTG12.272727272727273No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)