FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003647248

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003647248
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44
Sequences flagged as poor quality0
Sequence length146
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAGAGGGAAGGTTAATGGTTGATATTGCTAGGGTGGCGCTTCCAATT36.8181818181818175No Hit
GTTATGCTCCACTCTCAGTGCTGGTGAAACGTATGATGCGTAACACAGTG24.545454545454546No Hit
CATCCCGGGAGAGGCTGGCCAAGTCCTTCAGGGCTTCCAGCTTGGCATCC24.545454545454546No Hit
GGGTGGGACTCCAGGAGCCAGCTCTTACCCTAGAGTTTCTGCACGGGCAG24.545454545454546No Hit
AAACTAACCTGTCTCACGACGGTCTAATCCCAGCTCACGTTCCCTATTAG24.545454545454546No Hit
GTACATGGGGGGCGGAGAGGCCTGGCGCACAGGGCGAGGGCGGCTGCGGC24.545454545454546No Hit
CTGTTTGAACAAAGAGGCTTTGTGGCCCATTTTCCATCTCTGTGTCTCTG24.545454545454546No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTGGTGCATG12.272727272727273No Hit
GTAGGTAGGGACAGTGGGAATCTCGTTCATCCATTCATGCGCGTCACTAA12.272727272727273No Hit
GGAACGAGATCTTCACTTTCGCCGAGTCCGAGCTGGGTCTGGAGCACGGG12.272727272727273No Hit
CTTCAGATGAATCAGAGGTGCCAAAGATGACAGAAGCTGCTTCAGGTGGT12.272727272727273No Hit
CCGCCGGTTCGTCGCTGACGACGCAGCAGGCCGAGAACAACATCGCGCGC12.272727272727273No Hit
GTTCTGGGTCCTTTCATCATAAGGGAGAAGCTTCAGAAAGTTCCGAGGAC12.272727272727273No Hit
TCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCC12.272727272727273No Hit
CCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGG12.272727272727273No Hit
GGACATGGGGGGCGGAGAGGCCTGGCGCACAGGGCGAGGGCGGCTGCGGC12.272727272727273No Hit
AAATTAGAGTGTTCAAAGCAGGCCCGAGCCACCTGGATACCGCAGCTAGG12.272727272727273No Hit
ATTTCAAGATGACATTTAAAAATTATTCTAATATATCAGCAGCAAAAATA12.272727272727273No Hit
CCCAAGATCCAACTACGAGCTTTTTAACTGCAGCAACTTTAATATACGCT12.272727272727273No Hit
GATCCAGACCGCATGGTCCTTCTCATCGCGTCCCTTGGATCCGGTGGTGC12.272727272727273No Hit
CCCTTGCCCTTCATGGAGTCATACAGCCGATCAGCAAAATACAGGGGCTT12.272727272727273No Hit
AGGTGGTTCTCGCGTGCCGTTTGCAGAATAGAGCCAGCGAACCACCACAG12.272727272727273No Hit
GGCGTATCCTTGCAGCCTCACAACAGTGGGAGGTCTGTATCCTGAATGTC12.272727272727273No Hit
GAACACCTGCGCCGGGAGGTGTCCGCCGGGCTTCCGGTCGTGGCCTTCGA12.272727272727273No Hit
GATCTTGGCAGAGAGACATGCTTGTTCCTCTGGATTGGAAAGATGATTTA12.272727272727273No Hit
TGACTGGGAAGTTACTAAAACTGGGGTTCCAAGAAAAAACAGTGGATAAA12.272727272727273No Hit
GAATCACTTGAGTTATTGTAATTTAGTCATGACAATAACAGAACAGTTAC12.272727272727273No Hit
GCCCATTTTTTTGTCCTAAAAATCATCTGAAAAGTTTTGCCATCACATGT12.272727272727273No Hit
GCTCTTTCTCGATTCCGTGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTG12.272727272727273No Hit
GATCACGGTGATCGCCTACGTGCTGCTGGCCGTCGGATTCATCGGGCACC12.272727272727273No Hit
CCTCTGCCTTGGCCAGGGTGATCTCCTGGCGGACCAGGGTGGTGAGGTTG12.272727272727273No Hit
GTGGGACATGAGACCCGAGGCATCTTCGATCCAGTCTTCATTCTCCAGAA12.272727272727273No Hit
ATGCGGGGGTGGGATCTCAGCAGCCCGCCGAGTGCGCCGGAGAGGCAGCC12.272727272727273No Hit
TCTCAGGGTGGTGGCTGGGATGCACGAACTGGGAGTTTCCACCAAGGAGA12.272727272727273No Hit
CTGTAAGTGCTTCCTTGGAGTCTGTCAGGGGAAACAGGAGCCTCTTCACT12.272727272727273No Hit
CCTGGACACCTCAGAGCTCCTTAATAAGCATTTATAGTCAGTTTTTGGCA12.272727272727273No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)