FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003647255

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003647255
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences90
Sequences flagged as poor quality0
Sequence length146
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTGCCCAGGCCCGGCGGGCCGAAAAGCAGCACGTGGTCGAGGGCTTC44.444444444444445No Hit
ACCTGAAATAGCCTTTAAATTTTGACTTCTCCTGCAAGAAAGCATCTGCA33.3333333333333335No Hit
GTGGAGGCGAGTCAGGCCCGCGAAAAATTGATCCAGGAAGCCCAGTCCCG22.2222222222222223No Hit
CCGCAGCGCTGCCGTATCGTTCCGCCTGGGCGGGATTCTGACTTAGAGGC22.2222222222222223No Hit
ACTTAGAACTGGTGCGGACCAGGGGAATCCGACTGTTTAATTAAAACAAA22.2222222222222223No Hit
ACCGAGATCTACACTCTTTCCCTACACGACGCCTTCCGATCTATCCCTTT22.2222222222222223Illumina Single End PCR Primer 1 (96% over 32bp)
AGTCTGGCACGGTGAAGAGACATGAGAGGTGTAGAATAAGTGGGAGGCCC22.2222222222222223No Hit
GAGCGTGCCACTGCACTCCAGCCTGGGTGACAGAGTGAGACTCTGTTTCC22.2222222222222223No Hit
GTGTAAGAGAGCTGCTGCAGAATAAAACCGTATTCACCTGCTCATCCACC22.2222222222222223No Hit
GAGCTGAAGGATAGGAAAAGAGGATTTTCCCAGAAAGCCAAGGAATACTC22.2222222222222223No Hit
CCCCCTCCCCAGCCTTGCCCATGAGGTGCTCGAACACCTGCCTGCCCGAC22.2222222222222223No Hit
GACGTTGGTGGCGCTCAACGTGTTGATCGTGCCGGCGTTGGCCGTCAGGT22.2222222222222223No Hit
AACTACAGGCTCACGCCACTATACCCAGCTAATTTTTTTGTATGTTTAGT22.2222222222222223No Hit
GTGCATGTCTGTTTCTCTCTAATCCATATTTCCATTTACAGTTAGCAGAA22.2222222222222223No Hit
GGTTTTAATAGAGGACAGTGCCTTCACATATCCACATATACACTAAGTTT22.2222222222222223No Hit
ATCGTGAGATGAACGCCCAGCCCGCCCGGCTCGGGCACCGGGTAGACGAG22.2222222222222223No Hit
GGCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAA11.1111111111111112No Hit
AAGATTGAACCATGAAGAAATCCAAAATTTAAACAGACCAGTAATAAGTA11.1111111111111112No Hit
AGATAATCTTGTGGTTTTTGTGTTTGGTTCTCTTTATGTGATGGATTATG11.1111111111111112No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTACC11.1111111111111112Clontech SMART CDS Primer II A (100% over 21bp)
ACGCAGAGTACATGGGGTGGGCCATGCCGATGCCGCTTCCGAGCACCAGC11.1111111111111112No Hit
GGCAGGTACCAGAAAGTAAGTCCTCAGCAGGAAAAACACAAACAAAATTT11.1111111111111112No Hit
GGCTGTGGCTGAATGCCTCATGGATTCTTACAGCTTGGATGTCCATGGGG11.1111111111111112No Hit
GATTTATTTATAAGGCTTTGAGGCTTCTCTTACAAGAAGGGTCCCCTGCC11.1111111111111112No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTAC11.1111111111111112Clontech SMART CDS Primer II A (100% over 21bp)
GTTTATGGTAGGTTCTATTGCCATTTGGCCGGCATACCATTTACATTTCC11.1111111111111112No Hit
ATTATGATGTCAGCTGATCATTTTTCCCATTAATTGATGGCACTTTCTTC11.1111111111111112No Hit
CTCTCAGGGTCGGCCCTGCCAGGGACCAGCGATCCACCCTCAGCAAAACA11.1111111111111112No Hit
GATGGACACCGGCCGCGTGGTGGAGGGCGGCAAGGTCTACGACCTCTTCT11.1111111111111112No Hit
GAATTTCTCATTATTTAACACTACTTAAAGTAAGGGAGAAAAACACCAGT11.1111111111111112No Hit
CAATAAACCTATGAAAAAATGCTCAACATCACTAATGATCCAGGGAAATG11.1111111111111112No Hit
GTACATGGGAAGCACCAGATTACCTTTTCCCTACACGACGCTCTTCCGAT11.1111111111111112Illumina Single End PCR Primer 1 (96% over 26bp)
ACTTAGCAGAGACCCTGACCTCTCACCCCAACCCGATTCCAACTGCCCAG11.1111111111111112No Hit
ATTCAAACCTGCTCTATGATAGGGAATGTTCAACTCTGTGTCCTGAATAC11.1111111111111112No Hit
CAACAGCTCCGGCATCTACACCGGCCAGGCCGTCGGTGCGGCGCTGGGCG11.1111111111111112No Hit
CCTCTCCAGAGCCTTCCCATCACGCGAGAACTCCTCCTGCATGACAAACT11.1111111111111112No Hit
CCTCATCAAATGTAATAAAAGAGAATCACCGAATGCACTAAAATGGGATG11.1111111111111112No Hit
GTCCTGAAACACCCCTTTTGTAGTATCTGGAACTGGACTTTTGGAGCGCT11.1111111111111112No Hit
AACGTTCCTTTAGACAGAGTAGATTGGAAACACTCTTTTTGTGGAATTTT11.1111111111111112No Hit
GTTGATGAGCGCATTCAGCCACTATCTGGCGCAGGATTATCCGGAATCGA11.1111111111111112No Hit
CTCCCCCGCCGAGCACTCCTCCCTGCCGGGCCGACCCATGCCGCCGTCGC11.1111111111111112No Hit
ATCGTGAGATGAACGCCCAGCCCGCCCGGCTCGGGCAACGGGAAGACGAG11.1111111111111112No Hit
CGGTAACGCAGGTGTCCTAAGGCGAGCTCAGGGAGGACAGAAACCTCCCG11.1111111111111112No Hit
GGTTAATATTAATAGGGATAAGGTTAACCAACACCAGACTTCTCTAAATC11.1111111111111112No Hit
GCTTTGAGGACTATGGTAGTAAAGGAAATAGCTTCATATAAAAGCTAGAC11.1111111111111112No Hit
GGCAGGGGCTTGCCCTCGCCGCGCAGGCGATAGGCGCAGGTGGAGGGCAG11.1111111111111112No Hit
GTGGCGGGCACTTGTAGTCCCAGCTACTCGGGAGGCTGAGGCAGGAGAAT11.1111111111111112No Hit
TTCCAATACATTTCATGAAACTGCATTCCATTCCATTCTGTCCAATCGGA11.1111111111111112No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCTACAGAACTATCTCGTATG11.1111111111111112No Hit
GAGTTGAAAGTAGGTCTTCATTTTGCAGTCATCATCTGTACGAATTCTTC11.1111111111111112No Hit
GGGGAATTGCAATAGAGAAATACTTTAATTCATGCCGAGCTGGCTGAACA11.1111111111111112No Hit
GGATAAGATCTGGTGTTCTATATACTGGATCGTGACTATAGCTACTAATA11.1111111111111112No Hit
TATATGAGGTATCAAATGTGGTCAAACTCATGGGAAGTAGAATGGTGGTT11.1111111111111112No Hit
GTCATTTTTATACATTGGTCATCCACAGCTTGGAATTTTTATTTTTGTGC11.1111111111111112No Hit
CCTCATGACCACGCTTTCCTTCTCCCTCGCCCTTGGGGGAGAGGGAGCAA11.1111111111111112No Hit
AAATTAGAGTGTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGG11.1111111111111112No Hit
GTCGGTCCGCTTGGGCACGCGGCGCAGCATCACCATCAGGAAGCCGGCCA11.1111111111111112No Hit
CAGGAGTTCGAGACCAGCCTGGCCAACATGGTGAAACCCCGTCTCTACTC11.1111111111111112No Hit
CCTTTGAAACTCCTTTGTAATAATTTGATCAAGCTAACACACATTTTTCA11.1111111111111112No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTACA11.1111111111111112Clontech SMART CDS Primer II A (100% over 21bp)
GGGCGTGCGCACTTCGGACACGCTCGCGAAGGTGGCGCGCCCGCGCGGGT11.1111111111111112No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTACCA11.1111111111111112Clontech SMART CDS Primer II A (100% over 21bp)
CTTTCCTTTATGATGGATGATAGATAAGAGTGAACTTTGTGCTTTCCTTG11.1111111111111112No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC11.1111111111111112Clontech Universal Primer Mix Long (96% over 26bp)
TTATTAAATAGGGAATCCTTTCCCCATTTCTTCTTTTTGTCAGGTTTGTC11.1111111111111112No Hit
GCAGGGTGGCTCACGCCTGAAATCCCAGCATTTTGGGCTGTCTCTTATAC11.1111111111111112No Hit
GGGCGTGCGCACTTCGGACACGCTCTCGAAGGTGGCGCGCCCGCGCGGGT11.1111111111111112No Hit
TTATGTAGCTGTGTTGGATGGATACATTATTTTAAGAGTCCTGTCCAGAT11.1111111111111112No Hit
GGAAGTAGCCGAACTGGCTTGGACTTGTGGGGTTGAGGAAGAAGGCGCAG11.1111111111111112No Hit
TTTAAAAGGTGGTACTATTGTTCAGAAATTTGCACAGAATCAAAGGACTT11.1111111111111112No Hit
GCAGGCCTGATAGTGGTGAATTCTCTCAGCATTTGCTTTTGTGAAAAGGA11.1111111111111112No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)