FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003647256

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003647256
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences90
Sequences flagged as poor quality0
Sequence length146
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTAAACTCGTCGGCGCACACTTCCAGCAAATGACCATCCAGACAGAC44.444444444444445No Hit
CATTTAAAAACATCGAGTACCTCAAGTCAGTTTGCCTTGAAAAATATCAA44.444444444444445No Hit
ATGCTGGCGCTGAAGTCGCCCATCACGCGCGCCGAGTGCGGCGATGGCGC33.3333333333333335No Hit
CAACAATAGATTGCTGAAATTATTCAACCAAACCCTTCAAAAGATATACT33.3333333333333335No Hit
GGCAACAGAGTGAGACTGTCTCAAAAAAAAAAAAAAAAAAAAAAAAGAAA22.2222222222222223No Hit
GGTGACGAAGAAAGGTGAGGGACTGGCAGTGTGGTAGACCAGCACAGTGC22.2222222222222223No Hit
CTTCCGATCTATCCCTTTTTTGGTACCAACAGGCCCAAAAAAGGGATAGA22.2222222222222223No Hit
GGCCCCGCTTTCACGGTCTGTATTCGTACTGAAAATCAAGATCAAGCGAG22.2222222222222223No Hit
CTAATAGGGAACGTGAGCTGGGTTTAGACCGTCGTGAGACAGGTTAGTTT22.2222222222222223No Hit
GCTCCCTATTCCCTGAAACTCCTCCTTCACATAGCTTCCATAGGACCACA22.2222222222222223No Hit
GCTTGGATCCATTTTTCCCAAAAAGTGTGATCCCCAGGCCATAAACATTA22.2222222222222223No Hit
TGGTTCAATGCGGCGGTGCGGCGCTGATGACCGCTACGGCGTCGGCGCAG22.2222222222222223No Hit
GGTGCTTTGTAGACCTGGCGTGGCAGTAGCTGCTGCTCTTTGACAAGTGT22.2222222222222223No Hit
GATGCTCACTTGTCCTGGGAAGACTCCTGCCTGCCATCCTGGCCCCTTGC22.2222222222222223No Hit
ATCTGTCGTTGGTGTCCAAGAGCCTCGCGCTGGCGCGTCTTCCGCAGGCG22.2222222222222223No Hit
GACTAGAGTCAAGCTCAACAGGGTCTTCTTTCCCCGCTGATTCCGCCAAG22.2222222222222223No Hit
CCTGAATTCCTGGCTGAAGTCCTAAGCTTTGAGCTGATTAATCAGTTTTG11.1111111111111112No Hit
CAGGGGCCGTGACGGGTTTTACGCGGGGGCGCGCTTTCAGGGGGCGCGCC11.1111111111111112No Hit
CAACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCCGGAAGCTGCC11.1111111111111112No Hit
CGCATCGCCAAGTCAATCCTAAGCCAAAAGAACAAAGCTGGAGGCATCAT11.1111111111111112No Hit
TCCTACGCCTTCCTCGGCCCACTGATCGGCTCGTTGGTGCGTGTCGTCTG11.1111111111111112No Hit
TGGCACCATTGACATCCCCGAATTTTTGACTATGATGGCTAGAAAAATGA11.1111111111111112No Hit
CTGTTTTCCATAGTGTTTGTACTAGTTTACATTCCTACTAGCAGTGTAGA11.1111111111111112No Hit
CCGCGCCGCACCGGCAGCCCGTAGCGGTCCTCGTAGTCGGCCAGGTACGC11.1111111111111112No Hit
CTACCCATGATCCTTGTTCTCAATACTGTGAGTCTAGCTGTCAATTCTTG11.1111111111111112No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG11.1111111111111112Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACATATGCAAATCAATAAACATAATCCATCACATAAAGAGAACCAAACAC11.1111111111111112No Hit
ACGAAAAATTGAAAAATGCAATCAAAGTCAGTACCAGTGTTTGCGTATAA11.1111111111111112No Hit
GAGATGGGGTTCACCATGTTGGCCAGGCTGGTCTTGAAATCCTGACCTCA11.1111111111111112No Hit
TCGTTGATCAGATCGACCTTAAGCCGCGCATCGGCGGCGTACGACGTATC11.1111111111111112No Hit
GCACAATCTCGGCTGACTCTAGCCTCCACCTCCTGGGTTCAAGCGATTCT11.1111111111111112No Hit
ACCTAGTAAGACAGACCAACATTCAAATCCAGGAAATCCAGAGAACCCCA11.1111111111111112No Hit
CTACAAATAGTGTGTTTCGAAACTGCTCCATCCAAAGGGATGTTCAGCTC11.1111111111111112No Hit
CTCAGAAACTTACTCGTGATGTGTGTCCTCAACTAAAGGAGTAGAACCTT11.1111111111111112No Hit
GCATCACGCCAACCACACAGAGGGTGCTCATTAAATGCCACCTCGTTGAG11.1111111111111112No Hit
CAATCGGTGATTTCATTAGATTCCATTCGAGGATGATTCTATTCGAGTCC11.1111111111111112No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC11.1111111111111112Clontech Universal Primer Mix Long (96% over 26bp)
GGCCTGCAGTACACCTTCCGCCTGCGCCGGGGCGTGAAAAGGCACGACGG11.1111111111111112No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11.1111111111111112No Hit
GAGCAGATCGTCCAGCGCGACGAGAACTCATGGCTCATCGACGGCGTCAC11.1111111111111112No Hit
TACCTCTTCACAGTCAGCAATGCTTTCCAGGGGATGTTCATTTTTTTATT11.1111111111111112No Hit
GTGCTCACCGAATGGCTGGTGGACACCGCGCGCCATGCCGGCTATGCGGC11.1111111111111112No Hit
ACCATGATCAGCCGACCCTCGGTGCGCGCCCGGATGCGGGCCACCTCGTC11.1111111111111112No Hit
ATGTTTTCTCCGTAACATTTTTCTAAAGAGGTACCAGTAGCTCTGGGCAG11.1111111111111112No Hit
GGCCTTATAGAATGAGTTTGGAAGTATTCCCTCCTCCTCTATTTTTTTTT11.1111111111111112No Hit
CTTCTGCTCTCTTGATCTTTCATAGTACTGCAGACATTTTCTCATCAGAA11.1111111111111112No Hit
CTCTTGTTGCTTGGGCTGGAGTGCAATGGCACCATCTCGGCTCACTGCAA11.1111111111111112No Hit
AGACCCTGTCTCTACAAAAAAAAAAAAAAAAAAAGAAAGAAAAAAAGAAA11.1111111111111112No Hit
AGTCTTGAAGTGGGACTTATGATTGAAACGAAGGGTTTGAGAGAAAGTAT11.1111111111111112No Hit
GAATGGAAAGGAATGGACTCGAAAGAAATGGATTGGAATGGAATGGTCTC11.1111111111111112No Hit
GTCTGTATTCGTACTGAAAATCAAGATCAAGCGAGCTTTTGCCCTTCTGC11.1111111111111112No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11.1111111111111112No Hit
GGCCTGCAGTACACCTTCCGCCTGCGCCGGGGCGTGAAATGGCACGACGG11.1111111111111112No Hit
AACAAAAGCAATGGGAAGACAGTGAGATAACCCATTTCAATTGTGGGAAT11.1111111111111112No Hit
GCTCGACCGGGCGCAATGGGAAGCCCTTTGCGACGGCTGCGGCAAATGCT11.1111111111111112No Hit
ACATAAATGTTACCATCTTAACCATTTTTAAGTGTAGAGTTTAATTGTAT11.1111111111111112No Hit
GAAATCTGTGAGACAATTGATTAAATCTCTGAATGGTTCCTTATTTAACT11.1111111111111112No Hit
GAGCGCAACCAATGTCAGCACGACGGACCTGACCGCCAACGCCGGCACCA11.1111111111111112No Hit
GGCCGTGATGGCGCATGCCGACTGGATCATCGACCTCGGCCCCGGCGCGG11.1111111111111112No Hit
GTTTGGAAACACTGTGAGTTGAACACACACGTACCAAAGTAGTTTCTGAG11.1111111111111112No Hit
AGCCTGGGGAACACAGCGAGACTCTGTCTCAAAAAAAAAAATAAATAAAT11.1111111111111112No Hit
CCCAAAATGCTGGGATTTCAGGCGTGAGCCACCCTGCCTGTCTCTTATAC11.1111111111111112No Hit
GGAAAGAATTTTCAACCCAGAATTTCCTATCCAGCCAAACTAAGCTTCAT11.1111111111111112No Hit
ACGCTGCAGTCTGCAATTTGTATGAATTCCCGCTTCCAACGAAATCCTCA11.1111111111111112No Hit
GAATAAACTGTAAAAGAAAATAACATGGACATGTGAGTAGGCAGAAAAAT11.1111111111111112No Hit
CTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTA11.1111111111111112No Hit
GACTTACTTATTGCTTACTATGATGTGGACTATGAAAAGAACGCTAAAGG11.1111111111111112No Hit
GAGCGCAACCAATGTCAGCACGACTGACCTGACCGCCAACGCCGGCACCA11.1111111111111112No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)