FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003647273

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003647273
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences101
Sequences flagged as poor quality0
Sequence length146
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTAGGCAATACCATTCAGGACATAGGCATGGGCAAGGACTTCATGTCTA43.9603960396039604No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG32.9702970297029703Clontech Universal Primer Mix Long (96% over 26bp)
TTCTTCCACACCGTGACCCATTTGTGAGGGAAAGCATCCTTGGAGCGCTG32.9702970297029703No Hit
TCGCTCCACCAACTAAGAACGGCCATGCACCACCACCCACGGAATCGAGA32.9702970297029703No Hit
GAGTTTTTTGCAGCAAATGGAGCTAGAACAACTCTCCTTCCTTCATGGAA32.9702970297029703No Hit
CAGCGATAGCGATGGCGAACATGGATCAACCGTAATCGGTTGCAATCCAA21.9801980198019802No Hit
CTGCTTCCCAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC21.9801980198019802Clontech Universal Primer Mix Long (96% over 26bp)
CGCTAGGCGGGATTATGGCTAAGGCTAAAGATTCAATGCTCCATATTCGA21.9801980198019802No Hit
CCATCCTTTATTGCCTGAACCCTAAGAACTATGAAGACTACTACTAGAAC21.9801980198019802No Hit
GACATATACACCCTCTCAAGACTAACCCAGGAAGAAGTTGAATCTCTGAA21.9801980198019802No Hit
GACCACCGACCCTGGTACGACCGCCAGCGTCCCGATCTGTTCGCGGCGGC21.9801980198019802No Hit
GAAGATGACAGAAGGAAAAGTGATGTTCTTTGTGCAAGGATTTTTCGAAT21.9801980198019802No Hit
GTACGAATACAGACCGTGAAAGCGGGGCCTCACGATCCTTCTGACCTTTT21.9801980198019802No Hit
CCCAGAAACCAGCAGCTGTGTTTTGGAATCCTTTGTGAGGATAAACAAAC21.9801980198019802No Hit
GCTCTTAGTTTCGGTCACTCTCCAGAGACTCACAGCTGGATTTGGAAAAT21.9801980198019802No Hit
CCCAGTAGCCTCTGCTCCAGGGGGTGCGCTGGCTGTCGGGGGCTGGGCAT21.9801980198019802No Hit
AACCTCACCTTGGGGGCATCCTGGACCCCCGGCCTGGTGTGGTCACCAAG21.9801980198019802No Hit
TTTTCCTTTCCTTTATTTGGGCCTCCTCCTTCTATCTGGACCCTAGAATC21.9801980198019802No Hit
TCTCTTATCAGACCGTTTCCCGCGTGGTGAACCAGGCCAGCCACGTTTCT21.9801980198019802No Hit
CGCAGAGTACATGGGTTCCTGAAGCTGACAGCATTCGGGCCGAGATGTCT21.9801980198019802No Hit
GCTCTCCTGTATGAGGTGTCTGTTGACCCCTGCTGGGAGGTGTCTCCCAG10.9900990099009901No Hit
GAGTTTTTTGCAGCAAATGGAGCTAGAACCACTCTCCTTCCTTCATGGAA10.9900990099009901No Hit
GCACTGGGTAACTAAAAAACATAACTATATTGGAAAAACTGGGATAGTTT10.9900990099009901No Hit
CGGTAATGCTTCTGATAGTAATAGGTGGAACGAGGAATTCTGAGTATTTT10.9900990099009901No Hit
GTCCCCCTCGTCAGCCAGCCTGGGCATCAGGAGCTCCTCCGGCTCTATGA10.9900990099009901No Hit
CAAACGCATCTTCAGCACCGGCAATGCTTCGGCGGTGGCGCAAGACGTGC10.9900990099009901No Hit
GTATATACCCAAAGGAAAATAAATCATTCTATTATAAAGACACATGCACA10.9900990099009901No Hit
GTCGTAATTATTGATGTGGAAGCTGCAAAAGGCTGCAATACCCCATCCTT10.9900990099009901No Hit
GATTGCTTCTGGGATAAATTCTTGATCAAGCCAGTGATGCAAGCGCTGGC10.9900990099009901No Hit
ACTTAAACTGATGAGAGAGGAGAGGACAGAAAGGGAAGATGATGCAGAAT10.9900990099009901No Hit
GCTTATAGGGCACTTGATGACATTAGTGAAAGCATCAAAGAGCTTCAGTT10.9900990099009901No Hit
GAGGATGCTGCTGTCTACTTTTTATACGTAATCCCGTTTCCAACGAAATC10.9900990099009901No Hit
CCATAGTACCTTCCACCTCTTCCAAATGTTCTAATCACTGGGGTGGAAAT10.9900990099009901No Hit
TTTTCCTTTCCTTTAATTGGGCCTCCTCCTACTATCTGGACCCTAGAATC10.9900990099009901No Hit
CCTCAAGGCACTTAACTGAACTCCCAAAGGTTAAGGATAAAGAAATGATT10.9900990099009901No Hit
TGACATTTTAGGACACAGGATGAGAGTAGATGCCATTAAACCCTGTAAGT10.9900990099009901No Hit
TGGGTAATTAGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTC10.9900990099009901No Hit
GGGACGGTCAGAAGGCGTGTCCCTTGACCCCAAGCAGCAGCTGACAGCCG10.9900990099009901No Hit
GCCATTAACAGCTATCATCAACCTATGGAGTTCCCACGCCCAAAATACTT10.9900990099009901No Hit
ATGTATTTCCTTAACTCTTAAAAGGTTTTTGCTCACCTCTTTGCTCCCTG10.9900990099009901No Hit
CTCCACTCCACTCCATTCAATTCCATTCCACTCCTTTCCACTCTAATCCA10.9900990099009901No Hit
GTTCCATTGGTCTATATCTCTGTTTTGGTACCAGTACCATGCTGTTTTGG10.9900990099009901No Hit
AGGTGATCTAGAGTCACCAAAGCCGCCGGCGCCCGCCCCCCGGCCGGGGC10.9900990099009901No Hit
CACCACTACCATGTTTGAAAACTTTCAGGACTTACTTTATCTTTGGTATT10.9900990099009901No Hit
AATCAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATACCGTCGTAGT10.9900990099009901No Hit
CTCTGACTATGCTTCACGCATATTAGCAAGGAATGACATCTAACAAGTCA10.9900990099009901No Hit
GCATTCTCCGTCCTTACCCAAGCCATCACTGGCCAGGTGGGTTTTGTGGA10.9900990099009901No Hit
TGAAAGACCAGTATGAATATACCTTCAAAGCAGCAACACAGGTTCCCCAT10.9900990099009901No Hit
CTCTCATTCAATCATCATCAATGGAATTGAATGGAAAAATCATCAAATGG10.9900990099009901No Hit
ATATCAGTATTTAAAGAATTATAAACCCATGGTTTGAATGTCTTTATCTC10.9900990099009901No Hit
GCGCCACAGCAGGTCCAGGTACCGGCTGGAGGTGGCCAGCTCGGACTCCA10.9900990099009901No Hit
GACACGTTCTTGATCGAGTCGTTCGATCCGTTCGATGGCAGGGGCTTCGG10.9900990099009901No Hit
ACTCTATGCCAGTGGTTCAGAAGATGGAACATTGAGACTATGGCAAACTG10.9900990099009901No Hit
CACCTGCTCTCTGGCTGGGCTCTGGCACAGGAAGCCCTCAGCAAACACCC10.9900990099009901No Hit
ATACCTCAGGAAGACCAATTTTTTCAAAACAAAGGACAAAGTTAAAGACA10.9900990099009901No Hit
GAAAATTGTAAAAAGCTGTAGTTTAACCAACTCTAATTCCTAATCTTCCC10.9900990099009901No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC10.9900990099009901Clontech Universal Primer Mix Long (96% over 26bp)
ACTTCCTCCACTCTTGAGAAAGGGGATTGTTTCTGGTTGGAAGGGTAGGA10.9900990099009901No Hit
GCACTCTGTCCAGTAAATATGGAGGAAGGGAAACGTGAGCAGAGCTTTGT10.9900990099009901No Hit
TATCAACGCCGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG10.9900990099009901No Hit
CCTGTTCCGCTAACTTTTTCGCGAGCGCGGCGCGGCGGCGGTGATGGATC10.9900990099009901No Hit
GAAGGTACCTGTCTTAGTTATTTTCATAAGTCTATGTAGCTACACCTTGC10.9900990099009901No Hit
CAAGTATGTTGTTGCATAGGCTGTACAGTTGGGAAGTAGAAACCAAATGC10.9900990099009901No Hit
GCACTCTTTCCAGTAAATATGGAGGAAGGGAAACGTGAGCAGATCTTTGT10.9900990099009901No Hit
GAGCAGGTTTCAAACACTCTTTTTGTAGTATCTGGAAGTGGACATTTGGA10.9900990099009901No Hit
CATTAATAGTGGATGGGAGCATCTGACAGAAGTGAGATCAGGCAGTGGGT10.9900990099009901No Hit
CCATGAACTCGCTGTCTTCCAGCTTCCACTCATGCCCCTCGCATTCATTA10.9900990099009901No Hit
GGGGGAGACTATGATGTAAAACTAGGCTAATCCTGGCTACAGGTGACTGA10.9900990099009901No Hit
CCTCAGCACCCCTCCAGTTTTGCCTGAGAAGGGGCAGATGTTCCCGGAGC10.9900990099009901No Hit
GTGCAATCAAACTAGAACGCAGGATTAAGAAACTCACTCAAAACCACTCA10.9900990099009901No Hit
TTCCTCATCTGACTTGGGATTCTCAAATCCTCTTAAAATTCTCTCTCTTT10.9900990099009901No Hit
TCAGTAAGCATATAAAATTTCAGCAATCTTTTGGACCAGTAATTGATTTG10.9900990099009901No Hit
GTTCCATTGGTCTATATCTCGGTTTTGGTACCAGTACCATGCTGTTTTGG10.9900990099009901No Hit
GCATCGAATAGAACGGCGTGAAGTACCAGACCGGGGCGATGTGGTTCGGC10.9900990099009901No Hit
CAGTAATAGTGGATGGGAGCATCTGACAGAAGTGAGATCAGGCAGTGGGT10.9900990099009901No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)