FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003647274

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003647274
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences101
Sequences flagged as poor quality0
Sequence length146
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGGTACTGGGACATGGGGAGTCTAACCCGGATCTTGAAGTCCTTCTTTC43.9603960396039604No Hit
GGGATTGTCTGTTTTTTTCTTTTAAATTTGAGTTCATTGTAGATTCTGGA43.9603960396039604No Hit
ACACGGGAAACCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGAT32.9702970297029703No Hit
CCCCATTGCCCACCCAGAGTTCTTCTGGGGGAACCTCCACTATTCATTTC32.9702970297029703No Hit
AACCAAAACTGTCCCATCAGGGACCAGATGGCATTTGACTTTGGGGCTGA32.9702970297029703No Hit
GTGGCGGGGGAGCTGGCGGCGACCGAGCAAGTGCCGCTGAGCGCCCGGAT21.9801980198019802No Hit
CCCTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAAT21.9801980198019802No Hit
CCTTGTAGAGATTTTTCACCTCCCTAGTTATTTTGTGGCAATTGTGAATG21.9801980198019802No Hit
GAGCATTGCTGCTGGATTCAGAATGTTTGTCCCTCACATAGGATTCCAGA21.9801980198019802No Hit
GATGAAACCCAGACACATAGCAATTCAGGAAATTTGACTTTCCATTCTCT21.9801980198019802No Hit
ATATCGTGAAAATGGCCATACTGCCCAAGGTAATTTATAGATTCAATGCC21.9801980198019802No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC21.9801980198019802Clontech SMART CDS Primer II A (96% over 26bp)
GTCTCTGGGTGCTGAACTGGAGTGGAGGTAGCTAGGCGTGGGAGAGAAAT21.9801980198019802No Hit
AAGGACTGCGAGGTGGACTTTGTGGAGTATGTGCGCTCACTTGCCTGCCT21.9801980198019802No Hit
TAACGACGCGGGATAGTGACGCTAAGTTATGCAACTTGAGACTGATGAAA21.9801980198019802No Hit
GATCAGCCCTTCCATACCGCATACGAACAGGTTACCGAGTACAACGATCA21.9801980198019802No Hit
GTGGTATCAACGCAGAGTACTTGGGAAGCAGTGGTATCAACGCAGAGTAC21.9801980198019802Clontech Universal Primer Mix Long (96% over 26bp)
GGTTCACAGTGTCCACCGTGGAGGAGGAGAGGACTCTGAACCCTCGGCTC21.9801980198019802No Hit
GATTTCTAAAATGGATGTAAAAGCTGGTTATGATGTTGACAGCCAAAAGG21.9801980198019802No Hit
CATGGACGCCTCTGAGGAGCCGCTGCCGCCGGTGATCTACACCATGGAGA21.9801980198019802No Hit
GATTCCCTCTTTTTCTATTGATTGGAATAGTTTCAGAAGGAATGGTACCA21.9801980198019802No Hit
CCTGAAGCTCCGCCTGCTCCATGGACCAACTCTAGTAGCATTCGCCGCTT21.9801980198019802No Hit
ATTGTGCACCGCCGCTTTACAGGCTTCGACGCCGCTTCGTTCTACCATCG21.9801980198019802No Hit
AACCGGAACCGCTCTACTAGGCCTCTTCTCGCATGTCTTGCCAGGATCGC10.9900990099009901No Hit
CATTAAGAGGGAGCAGCAGTCATGGCTTATTCACCTCTTAAAGGCCCCAC10.9900990099009901No Hit
GCGACCAAAGGAACCATAACTGATTTAATGAGCCATTCGCAGTTTCACTG10.9900990099009901No Hit
GTGCTATACTGCATACTTTTTAAATGTAAAGATATTTTTATCTTTATATG10.9900990099009901No Hit
GATCTTAGTTATTTCTTGCCTTCTGCTAGCTTTTGAATGTGTTTGCTCTT10.9900990099009901No Hit
GGGCACACCTTCAGAGGACTTCAACATCCCTGCTTGCCAGCTCTGAAGAA10.9900990099009901No Hit
GTAATAAATAAGATGCCAAAGAAGAAACTCTCTTTAAGAAAAGAAAAGAT10.9900990099009901No Hit
TCTTTTTTACATGGTGAAAATGTGGCCCTGACAGTTTTAAGTTTTACACC10.9900990099009901No Hit
TTGTGTTCCAATTTAGCATTTTCAGAAACTTAAGTAAAATGCATTTTCAT10.9900990099009901No Hit
GCATTTATCAGATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCC10.9900990099009901No Hit
GTTGTGTGCCACAATGCCCGACTAATTTTTGTATTTTTTAGTAGAGATGG10.9900990099009901No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC10.9900990099009901Clontech Universal Primer Mix Long (96% over 26bp)
CCCTAAGAAACAATGCCTAATCAAGCACCAATTTACCACACATAATGCCC10.9900990099009901No Hit
CCTTGACGCCTACGGTGAAAAGGGAAATATCTTCCCATAAAAACTAGACA10.9900990099009901No Hit
GCTGCTGGCCGCCGACGAGCGACCGGCGATCGACGCGCTGCGGCGCGCCG10.9900990099009901No Hit
AATTTGATACGGTCGAAATCGAGGAGCGGCTCGAGACCTTCCAACCGGAC10.9900990099009901No Hit
CTTGGTGGATTCTTGGGTCCCTGTGGGACCTTGTGCATGCATGGTCCTCT10.9900990099009901No Hit
ATTGTGAATAATGCTGCAATAAACATACGTGTGCATGTGTCTTTATAATA10.9900990099009901No Hit
CTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCAACC10.9900990099009901No Hit
CAATGAAGATTGCATTCGATTCCATTTGATGATGATTCCATTCAAAACCA10.9900990099009901No Hit
CTGCCAAGCCATCAGCGTGAGAAAAAAAAAACCACCAGAAGTTGCCTCCA10.9900990099009901No Hit
GAGTAAACCAGTGTGTTTTCTGGCAGGTTTGATCACAATATATTACCATT10.9900990099009901No Hit
AAATCGAATTAAATTGAATGGATTCAAATGGAATTGAATCAAATGGAATG10.9900990099009901No Hit
GCATAACAATAGGTAAAACACACCCTGTGGTTTTCCCAAAGGTTACTGAA10.9900990099009901No Hit
CTACTATTAATTCCGATAAATCATGCACATAATGGATTCAGGTCATCCCT10.9900990099009901No Hit
CAGTTGGACCTCACAATGTGGATTGTGCCCTCACCCAGCAATGTTTACGC10.9900990099009901No Hit
ACCTCAGCCTCCCAAGGTACTGGGATTGTAGGTGTGAGCCACTGCATCTG10.9900990099009901No Hit
CCCAAAGTGTTGGGATTAAAGGTGTGAGCCACCGCGCCTGGCCTATTTTT10.9900990099009901No Hit
CTACGAAAGTATTTTTTGGCAACCTGAGTCTTTAATATAAAAATAAAAGA10.9900990099009901No Hit
GCCGTGGCCGACGCACTGAGCGCATTGAAGAACTGCGTGGACGCGCGCAA10.9900990099009901No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT10.9900990099009901Clontech Universal Primer Mix Long (96% over 26bp)
GTCTCAAAATACATAAGGCAAAATGACAGCAAATATATTTAATATGGTTA10.9900990099009901No Hit
GAATGCTTCTGCCTAGTTGTTACGGGAAGATATTTCCCTTTCCAACATAG10.9900990099009901No Hit
GTGAGAACTACATTTAGAGTAACCATTTAGATAAATTTTCCAGAACTAGA10.9900990099009901No Hit
GGCACTGTGCCTGGCGCTTTATGCACAGTTCTCTTTCTCATCATCATGAG10.9900990099009901No Hit
CAGCAATAACTGGGAGCAGCCAGCAGAATGACTGTTGCCAAACATTTCCA10.9900990099009901No Hit
AGACCAGATCATGCAGGGCGTTGAGGTTATAGAAAGGAGTTTAACTATTG10.9900990099009901No Hit
ATTTTATGGTTTTTCAGCAAAGTGTGTCTACAATTAGTCTTAAAGTGAAA10.9900990099009901No Hit
TGCCTTCCGTGTGGCGGCAGCATCGGGACACTGCGGAGCCTTCTCAGGCA10.9900990099009901No Hit
CACCGGCGGCCACCAAGTGCCGGAAGCGGAAGCTGGAGCGCATCGCGCGC10.9900990099009901No Hit
TCTGCAAGGTGTGCCTGAGGGTCAGTTCCCTGAGATGTGACAACAGCGAG10.9900990099009901No Hit
GAAATTACTTGGTGAAAAAGACCTTGAAATTGCCATTTTGCGAGATCTCA10.9900990099009901No Hit
CTCTGAAGCAATTTCTTCTAGCTCCTCTTCTGTTGTCTCTGGAAAACCAA10.9900990099009901No Hit
CCCTGGGGAGGGGGATAAAGCCCTGATTTGTAATGTTTGCTGATTTCTGA10.9900990099009901No Hit
GCACTCGGCGAGACGAGCACAGGTTATCAGCAAGTTGGAGCGATCGCCTT10.9900990099009901No Hit
GTCTAGAAGTCCCTTTGGCATTTCCTGTAGTAAAGGTCTGGTGTTAATGA10.9900990099009901No Hit
GCACGAAGTGGGTTAGAACAACCCCGACGGCATCGAGATCAAGGCCAAGC10.9900990099009901No Hit
ACTTTATGTGCACTGAGCTGCAACTTCTTACTTCCCTACTGAAAATAAGA10.9900990099009901No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)