FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003647370

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003647370
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52
Sequences flagged as poor quality0
Sequence length146
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG35.769230769230769Clontech Universal Primer Mix Long (96% over 26bp)
GCCCAAGGCGCCGCGATCAACCAGGCCCTGGGCCGTACCGCGCTGCTGGT35.769230769230769No Hit
TGCGCGGTCCGGCCGCGTGGCCGGTTCCTGCGGATGCGGAACGAATGAAG23.8461538461538463No Hit
CGAGCAGGCTGCGCACCACCTCGGCGTCCAGGTAGGTGGCGAAGACGCGG23.8461538461538463No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT23.8461538461538463Clontech Universal Primer Mix Long (100% over 23bp)
GATACAGGCTCAGCAAAGTGGTGTGCCCCAGCAAATCAAACTCCAGTTAC23.8461538461538463No Hit
TTACAGCAAATACCAGCGGCCCTGACCCCGGGGAACAGTCGCATGACGAG23.8461538461538463No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT23.8461538461538463Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA11.9230769230769231Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTATGACTCTCTTAAGGTAGCCAAATGCCTCGTCATCTAATTAGTGACGC11.9230769230769231No Hit
GTGTGCTTCGTTTTCGATTTCGCCCCTACTCGCGCTTTGCGGCAACTATC11.9230769230769231No Hit
GGGTTTGAGGGTGATTGCGTAACCTGACGCCTGACTTTAAGGAAGTCAGG11.9230769230769231No Hit
GTACATGGGTGGCGACAGCGAGGCAAAAAAAAAAAAAAAAAAAAAAAAAG11.9230769230769231No Hit
GAACATGCCGGCCGCGGAGATCCTCAGGCGCTGCGACGGCCGGACGGCGG11.9230769230769231No Hit
CGTCTACGCCTACTGGGCCGCCGTCGACCAAGGCCTCTGCGAGGCCGTCA11.9230769230769231No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT11.9230769230769231Clontech Universal Primer Mix Long (96% over 26bp)
CTGCTGGGCGAGTCCCTGCGCGAGGCCCTCGACCCCCTGTCTCTTATACA11.9230769230769231No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG11.9230769230769231Clontech Universal Primer Mix Long (96% over 26bp)
GTATAAAATCAGACAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC11.9230769230769231No Hit
CAACCACATTCATCTTGATCTCGAAGATATTGATAGCGTCAGTGACAGCC11.9230769230769231No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA11.9230769230769231Clontech Universal Primer Mix Long (96% over 26bp)
TCATAATTTGGGGTAAAAATGCGTAAATCAGCAACCGCTTGAACGCCATG11.9230769230769231No Hit
CACCGACCTCGCCGACCAGACCGGGGCCCGCTCGGTGGCCTCCTGGCCTG11.9230769230769231No Hit
TTGCCGACTTCCCTTACCTACATTGTTCCAACATGCCAGAGGCTGTTCAC11.9230769230769231No Hit
CGGCGAAGAGCGGCCGGTCCTGCGCCGCGTCGCTGCGCTGCAGCTGCCAG11.9230769230769231No Hit
CATCAATGCGATTGATTATAACGATAAAAAAGTACTGGATTACATTGGAA11.9230769230769231No Hit
TCGTCGCTCTTCGTTGTTCGTCGACTTCTAGTGTGGAGACGAATTGCCAG11.9230769230769231No Hit
CTCGCAGACCCTCGCTGCGGTCGGCCTCAGACACCCAGTTGCGGATGGCC11.9230769230769231No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA11.9230769230769231Clontech Universal Primer Mix Long (96% over 26bp)
GTACGAATACAGACCGTGAAAGCGGGGCCTCACGATCCTTCTGACCTTTT11.9230769230769231No Hit
CAGGATACATATATATTTAGAGAAGGAAATATGAAATCAAGAGTTTTGGC11.9230769230769231No Hit
GGTCCGACATCCGGATCGGGAACCGCACGGCACAACCGAACCAGCGCTCC11.9230769230769231No Hit
CGCCCAATGCGACGCCTTGAGCATGATTGCCGGCCGAGGCGCAAATCACG11.9230769230769231No Hit
TTATAGGTGTACAGCGGCTTGCCTTTGTAGACCCACTGAGAAGCCTGTCT11.9230769230769231No Hit
CTATAAAGGTGCCACCCCCAGCAGGGGTGCTGCCCGGTACCTTGTGGGCA11.9230769230769231No Hit
CCGCCCGCTCGGCGCACTGGACAGCGAGCAATGCCTGAGCCTCGCCGCCG11.9230769230769231No Hit
GTGGAGGACCCGAAGTCCTCCAAAGTCGCCGGGCAGACCGCTTACGCCCC11.9230769230769231No Hit
GTTCGATGTCCAGATCAGTGATGGCGTTGACCTCGGCAATGGCAGGCTTG11.9230769230769231No Hit
ATACAAAGCCACAGGCAGCGGCCGAAGCTAAAAGGAGCGCGAAGCCGGCA11.9230769230769231No Hit
GTACATGGGATGATACGGCGACCACCGAGATCCTGTCTCTTATACACATC11.9230769230769231Illumina Single End PCR Primer 1 (95% over 24bp)
CGATGAGCGAGGGGACCGAACACCGGCGTCGGTTCGGCAGGTTCCGTCCG11.9230769230769231No Hit
ATGCGTGGCGGTTTCGGCGGCGTGGTCAGCTTTGAAGTGAAGGGTGGCAA11.9230769230769231No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)