FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003647371

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003647371
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50
Sequences flagged as poor quality0
Sequence length146
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACCGACTACATGATCGAGGCCGCGCGCACGCACAGGAATGCGGTGGTCA48.0No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG36.0Clontech Universal Primer Mix Long (96% over 26bp)
GTACTGGGTTGTCTGAACAACGCCACCACAGATGCCTGTGATGACTGAGA24.0No Hit
GACGCTTTCCAAGGTGAACAGCCTCTGGCATGTTGGAACAATGTAGGTAA24.0No Hit
TAATAGAATAGAGAAGATTTATTAGTGACACCTCATTTATCTGGAGGTAA24.0No Hit
GCCGCTGACCGACGCCGACATGCAGGCCGCGCCTGCCGAGCAGATGCGCT24.0No Hit
CACCACGGGCCTGGCCGTGGCTGCCCAGGCCACGCACGTGATGGCACGGG24.0No Hit
ACCTACGCACACGAGAACATGCCTCTCGCAAAGGATCTCCTTCATCCCTC24.0No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTAT12.0Clontech SMART CDS Primer II A (100% over 26bp)
GGTCGGTGAACAGCTCGTACTTGATCGCGGTGGAGACCACTACCTGGCCG12.0No Hit
GCATTTTATCAGTAGCAATGTAAGGATCTTATATATGTATCCCAGTTCAA12.0No Hit
TTCGTCGGCCATGTTGCGGCCACCCGCCACCGCCGCCACGTTGTCGGCCA12.0No Hit
CATCGAATGCCTCCGCCTGGCTGTGCGAGCAGCCGACGGAGCAGGGCACC12.0No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT12.0Clontech Universal Primer Mix Long (96% over 26bp)
CCGGCGAGGAGGACGAGGTGCGCTTCGGCCTGACGTCCGAAGCGGGCCTC12.0No Hit
ACCAGGCCCAATGCCCCTCTCATCTGACCCCACAGGGCCTGTCTCTTATA12.0No Hit
GATCTCGGTGGTCGCCGTATCATTCCCATGTACTCTGCGTTGATACTGTC12.0Illumina Single End PCR Primer 1 (100% over 24bp)
CCACGAAGTAGTCGCGCTCCGCCGCCGTGCCGTGAAGGACGATGCTGAAC12.0No Hit
GGCGCGGGCCGAGAGCGCCGAGGTAGATGGGCGGCGGGCCGTACGGGCTG12.0No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA12.0Clontech SMART CDS Primer II A (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG12.0Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CACCGACTACATGATCGAGGCCGCGCGCACGCACCGGAATGCGGTGGTCA12.0No Hit
GTACATGGGCAAGCAGAAGACGGCATACGAGATCTTGCTTCGTGACTGGA12.0Illumina PCR Primer Index 1 (96% over 28bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT12.0Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CGTTTTTCAGGACCTGTTGGTACAGATCGGAAGAGCGTCGTGTAGGAGTA12.0Illumina Single End PCR Primer 1 (95% over 23bp)
GCGGGGCGATGATGAGCAGTCGTGATCCAGCCGTCTGACCGGCCAGACCC12.0No Hit
GTGCTCGCGGCCGGTGGGCGCGTGCGGGAAGAGCTGCTTGCGGCGCTCCA12.0No Hit
GAATAAGACTGAAATAGTCCCAATACGCCCCGCTTGGCCGCAGTGCGAAC12.0No Hit
ATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAAACTTCA12.0No Hit
CCGATATGCTGGTTGCGGTCACCAATTCCGATGAAACGAATATGGTCTGT12.0No Hit
ATGTCTGTCTCGGCGCCGACTGGGATGTGGGCGGCGGTGTTATCGGCATG12.0No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTAGGCAC12.0No Hit
GAGCCCTCTTTACGTGATTCAAACCCAGAAGAGATTGAGATTGATTTTGA12.0No Hit
AGCCACGAGTCACGGGGTCTAACGCCTACCAGTACTGATTGCCGAGCAAA12.0No Hit
CTGTAGCCGCTTGGCAGGACCATTTTTCTGGTCATCGGGTCCAGCGTGGC12.0No Hit
AGGTAGGAATGGGAGTATTTGCACTTGTGGTAACGGTATTTGCATTATTG12.0No Hit
GTCGAGGCCATGGACACCGGCCACCTCGACGAAGCCACCGCCACCCTGCT12.0No Hit
AAGCTGATGCCTTCGCCCAGGCAGGGAAAACTGCCGTATTGCAGAACATC12.0No Hit
GTGGTGGCGCCTCATCAGCTGTCGGTAGGCAGCGTCGACGGAGGCTGGAT12.0No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)