FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003647372

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003647372
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50
Sequences flagged as poor quality0
Sequence length146
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGCCGTCATTCGCGTCGCCGACCAGCTCCTGCGTCTGCGCACCCAT48.0No Hit
TGTTGATCATACATATTCATTGGGTCTTGGGTTTTTTTCAGCCTGCTCAC24.0No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT24.0Clontech Universal Primer Mix Long (96% over 26bp)
GTATTGTCACAGGAATCATTGGTGCAGAAATTGCCATAATCACAATCTGG24.0No Hit
CGCCGAAATCGGTCGAGCGGTAGGTGGTGGTTGAATTTTCCGGACTATGC24.0No Hit
AAGTTACGGATCCGGCTTGCCGACTTCCCTTACCTACATTGTTCCAACAT24.0No Hit
GCATCAGCGGCCCGGCCGGCGATGAGCAGCCGGACGGCTGGCAGCGCCAC24.0No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA12.0Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTTAT12.0Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTACC12.0No Hit
GACCAACGGCAACGCGCTGGCCGGCCGTCTCGCCAAGGCCGCGATGGACA12.0No Hit
TCCGCGCGCGCTGGTGCGCTGGGCGAAGCCGACCGCGTCGCGCATCGCCT12.0No Hit
TATCAACGCAGAGTACATGGGAATGATACGGCGACCACCGAGATCCTGTC12.0Illumina Single End PCR Primer 1 (95% over 24bp)
CAGTGGTATCAACGCAGAGTACTTGGAAGCAGTGGTATCAACGCAGAGTA12.0Clontech Universal Primer Mix Long (96% over 25bp)
TTTTAGTAGAATTGAGCTGTCCGCTGACATCTTGTAACCGCTTTTCTAAT12.0No Hit
CCTGCCGGCGGCGAGGGCGGCCAGGAGCCAGTCGATGACCTCGCGGTGGA12.0No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAC12.0No Hit
CCGTGGCAGGTCGGCGGCATTGTCCATGCCGATAACACCGCCGCCCACAT12.0No Hit
GTTTTTATATCTATAAAAATTATAAATTTTAATATAATCAAATATCCAGT12.0No Hit
GCGAATGACAGTGGAAAGCTGTGTGTTGATTTCATGAATGCGATTTCTGA12.0No Hit
CTCCTGCCGTCATTCGCGTCGCCGACCAGCTCCTGCGTTTGCGCACCCAT12.0No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC12.0Clontech SMART CDS Primer II A (96% over 26bp)
GTTCAGCATCGTCCTTCACGGCACGGCGGCGGAGCGCGACTACTTCGTGG12.0No Hit
GTGTCCGGGCAGCGTTCCCCCTACAGCGTCCTCCGCCTGAAGCCCGGAGC12.0No Hit
AGCCGATACCGTGAAAGAAGAAAGAAAAACGGTGTGCTTCAAATTGCGCT12.0No Hit
ACCATATTCGTTTCATCGGAATTGGTGACCGCAACCAGCATATCGGCTGT12.0No Hit
CTACACCACCGGCGAGGACGACCTGCTCCTCGCACTCGCCAGCTACCCGA12.0No Hit
CTCTTATACACATCTGATGCTGCCGAAAAAAAAAAAAAAAAAAAAAAAAA12.0No Hit
GTACATGGGCGGCGGATACTCCTACACGACGCTCTTCCGATCTGTACCAA12.0Illumina Single End Sequencing Primer (95% over 24bp)
GCCCTGTGGGGTCAGATGAGAGGGGCATTGGGCCTGGTCTGTCTCTTATA12.0No Hit
GCTTTACGCTTTCACGCTTCGGTGGATTTGTTCCAAGAAAGGGCTGCCCA12.0No Hit
CCCCTGCTGGCCTGCCCACCGTCAGCATGGCCAAGGACGGCCCCAAGGTG12.0No Hit
GCTTGACGATATAGAAGTGCTCGACCGGCCCGCCCGACGGCTTGAGGATG12.0No Hit
CAATGATTCCATATAACGGCCATAATGGAGCTATAGAATACAACACCAAC12.0No Hit
TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG12.0Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT12.0Clontech Universal Primer Mix Long (96% over 26bp)
TGATTGCACAACCTGCGATCACCTTATACAGAATTATCAATCAAGCTCCC12.0No Hit
CCATCGGCCTCGGCCAGGTAGTGGTCTCCACCGCGATCAAGTACGAGCTG12.0No Hit
GGCCAGGGTTTTCCAGAGGACGCTCTGCAGCAGCCACTCCCCGGCCCAGG12.0No Hit
CATCCAGCTGGCGCACCAGGCCCACGACCTGCAGACGCTCAGCGAGGGCC12.0No Hit
TTATCAAAGAATAGTAATTTTTTTATTTTTTTATATTTTTTTTTTTAGTA12.0No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)