FastQCFastQC Report
Tue 10 May 2022
EGAF00003647539

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003647539
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences214561
Sequences flagged as poor quality0
Sequence length146
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTGTAGATGCTCCAGTCTCGGCTCAGCCAAACACTGTCAGGGCCCCCAG19370.9027735702201238No Hit
GTCCTGCAGCTCCGCTGCTGCTTTCTTAGTGGGCTCGGCCAAGCCTGTGG12330.5746617512036204No Hit
GTTTTCAGCCCTCGCGGGTGGGCAGCGTCTTGTCCTCAGGTGTAGATGCT10990.5122086492885474No Hit
TTATTGAAGAGAGCAAGGCTGGTGGGCGTGGACTCAAAGCATGGCAGCGG10830.5047515624927177No Hit
CCCTCACCCTGGTCACACTGGCTCTCTGCTGCAGCTCCGCTTCTGCAGAG10490.4889052530515798No Hit
GTTGGGGATCTTCAGCTTCTAAATGCTAATTACACAGTGAGCTTTGGGCT9080.42318967566333116No Hit
GGCCAGAGCATCCCCCTCCTCCACCATGAAACTCGCTGTCACCCTCACCC9030.4208593360396344No Hit
CTTCTGGGAGCCCTCTATGAGGTGGTTCACGGGGATGCCCAGGCTGCTCA8930.4161986567922409No Hit
GTCCTCCCCGCGGCGGGGTTTTCAGCCCTCGCGGGTGGGCAGCGTCTTGT8860.41293618131906545No Hit
GAGCAAGGCTGGTGGGCGTGGACTCAAAGCATGGCAGCGGCAGAGGCTGG8850.41247011339432604No Hit
CTCCTGGGGCTCTGCGTGGCCCTGTCCTGCAGCTCCGCTGCTGCTTTCTT8660.4036148228242784No Hit
TAATTACACAGTGAGCTTTGGGCTATTTTTTCCATGAGCTTAATGATGCT7320.3411617209092053No Hit
CTATGAGGTGGTTCACGGGGATGCCCAGGCTGCTCAGCAGGAGCTTCAGC6840.31879046052171645No Hit
GGGATGGACGGTCCTCCCCGCGGCGGGGTTTTCAGCCCTCGCGGGTGGGC6760.3150619171238016No Hit
ATTGAAGAGAGCAAGGCTGGTGGGCGTGGACTCAAAGCATGGCAGCGGCA6680.31133337372588676No Hit
GTCTTGTCCTCAGGTGTAGATGCTCCAGTCTCGGCTCAGCCAAACACTGT6660.3104012378764081No Hit
GGCTGGAGCAGTTGGGGATCTTCAGCTTCTAAATGCTAATTACACAGTGA6460.3010798793816211No Hit
GCCCTGTCCTGCAGCTCCGCTGCTGCTTTCTTAGTGGGCTCGGCCAAGCC6100.2843014340910044No Hit
GCGTGGCCCTGTCCTGCAGCTCCGCTGCTGCTTTCTTAGTGGGCTCGGCC6010.28010682276835025No Hit
TTCTTAGTGGGCTCGGCCAAGCCTGTGGCCCAGCCTGTCGCTGCGCTGGA5690.265192649176691No Hit
TCCTGGGGCTCTGCGTGGCCCTGTCCTGCAGCTCCGCTGCTGCTTTCTTA5680.2647265812519517No Hit
CCCCGAGCCCCCGCGCCATGAAGCTCGCCGCCCTCCTGGGGCTCTGCGTG5600.26099803785403686No Hit
CTCCTCCACCATGAAACTCGCTGTCACCCTCACCCTGGTCACACTGGCTC5410.25214274728398917No Hit
GGTCACACTGGCTCTCTGCTGCAGCTCCGCTTCTGCAGAGATCTGCCCGA5370.2502784755850318No Hit
GCCAAACACTGTCAGGGCCCCCAGCAGGGCCTTCAGGGCCTTCACGGCCC5210.24282138878920215No Hit
GTCCTCAGGTGTAGATGCTCCAGTCTCGGCTCAGCCAAACACTGTCAGGG5170.24095711709024473No Hit
GCTTAATGATGCTTTCTCTGGGCTTTTGGGGGAGGGTGTCCACCAGCTTC5150.24002498124076604No Hit
GGTCTATAGAGCTCACACCCATACTCCCAGCTGGGACCCAGCCTTCCCTG5010.2335000302944151No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG4970.2316357585954577No Hit
CCTGGGGCTCTGCGTGGCCCTGTCCTGCAGCTCCGCTGCTGCTTTCTTAG4910.2288393510470216No Hit
GGCCAAGCCTGTGGCCCAGCCTGTCGCTGCGCTGGAGTCGGCGGCGGAGG4870.22697507934806418No Hit
GTTATGAGGCTGCCATGGAACTTTTCAGCCCTGATCAAGACATGAGGGAG4460.20786629443375076No Hit
CTCCAGTCTCGGCTCAGCCAAACACTGTCAGGGCCCCCAGCAGGGCCTTC4460.20786629443375076No Hit
GTACATGGGATACTAGCCCACCAGACTCAGAGACGGAACCAGAGACGGGC4440.20693415858427208No Hit
CACTGGCTCTCTGCTGCAGCTCCGCTTCTGCAGAGATCTGCCCGAGCTTT4430.20646809065953273No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGATGCGTG4300.20040920763792114No Hit
ACTCAAAGCATGGCAGCGGCAGAGGCTGGAGCAGTTGGGGATCTTCAGCT4220.19668066424000635No Hit
TCTAAATGCTAATTACACAGTGAGCTTTGGGCTATTTTTTCCATGAGCTT4210.19621459631526697No Hit
GAACCAGAGACGGGCCAGAGCATCCCCCTCCTCCACCATGAAACTCGCTG4200.19574852839052762No Hit
CCTCATGGACACACCCTCCAGTTATGAGGCTGCCATGGAACTTTTCAGCC4130.19248605291735216No Hit
CTTCTGCAGAGATCTGCCCGAGCTTTCAGCGTGTCATCGAAACCCTCCTC4080.19015571329365544No Hit
GCTTGTGGTTTATTGAAGAGAGCAAGGCTGGTGGGCGTGGACTCAAAGCA4080.19015571329365544No Hit
AGGCTGGTGGGCGTGGACTCAAAGCATGGCAGCGGCAGAGGCTGGAGCAG3920.18269862649782578No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT3900.18176649064834707No Hit
CCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAGACGTTCG3900.18176649064834707No Hit
GTACATGGGAGCCGCAGGTTCCCCGCGCGCCCCGAGCCCCCGCGCCATGA3830.17850401517517164No Hit
GCCTGTCGCTGCGCTGGAGTCGGCGGCGGAGGCCGGGGCCGGGACCCTGG3820.17803794725043226No Hit
GTCAGGGCCCCCAGCAGGGCCTTCAGGGCCTTCACGGCCCCCACGGCCTG3790.17663974347621422No Hit
CTCCAGTTATGAGGCTGCCATGGAACTTTTCAGCCCTGATCAAGACATGA3730.17384333592777812No Hit
AGACAGGCCAGAGCATCCCCCTCCTCCACCATGAAACTCGCTGTCACCCT3670.171046928379342No Hit
GGTGGGCAGCGTCTTGTCCTCAGGTGTAGATGCTCCAGTCTCGGCTCAGC3660.17058086045460266No Hit
CTGCGTGGCCCTGTCCTGCAGCTCCGCTGCTGCTTTCTTAGTGGGCTCGG3620.16871658875564524No Hit
ATTACACAGTGAGCTTTGGGCTATTTTTTCCATGAGCTTAATGATGCTTT3580.16685231705668785No Hit
CTGTCAGGGCCCCCAGCAGGGCCTTCAGGGCCTTCACGGCCCCCACGGCC3560.16592018120720914No Hit
GCTAATTACACAGTGAGCTTTGGGCTATTTTTTCCATGAGCTTAATGATG3520.16405590950825175No Hit
CTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCATGGTAGGC3510.16358984158351236No Hit
CCATGAAGCTCGCCGCCCTCCTGGGGCTCTGCGTGGCCCTGTCCTGCAGC3500.16312377365877304No Hit
GCTTTCTTAGTGGGCTCGGCCAAGCCTGTGGCCCAGCCTGTCGCTGCGCT3440.16032736611033693No Hit
GGCCCAGCCTGTCGCTGCGCTGGAGTCGGCGGCGGAGGCCGGGGCCGGGA3400.1584630944113795No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT3400.1584630944113795No Hit
GACTCAAAGCATGGCAGCGGCAGAGGCTGGAGCAGTTGGGGATCTTCAGC3390.15799702648664016No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGA3380.1575309585619008No Hit
GCTGGTGGGCGTGGACTCAAAGCATGGCAGCGGCAGAGGCTGGAGCAGTT3320.1547345510134647No Hit
TGCTTGTGGTTTATTGAAGAGAGCAAGGCTGGTGGGCGTGGACTCAAAGC3310.15426848308872534No Hit
CCATGAAACTCGCTGTCACCCTCACCCTGGTCACACTGGCTCTCTGCTGC3300.153802415163986No Hit
TGCCCAGGCTGCTCAGCAGGAGCTTCAGCGGGTTGAGGGTGCCGAGGGGG3200.14914173591659247No Hit
GAAGAGAGCAAGGCTGGTGGGCGTGGACTCAAAGCATGGCAGCGGCAGAG3150.14681139629289575No Hit
CTGTCACCCTCACCCTGGTCACACTGGCTCTCTGCTGCAGCTCCGCTTCT3140.14634532836815636No Hit
CTGCTGCAGCTCCGCTTCTGCAGAGATCTGCCCGAGCTTTCAGCGTGTCA3110.14494712459393833No Hit
CTTCAGGGCCTTCACGGCCCCCACGGCCTGGGGACCCAGCTCAGCCACAC3100.14448105666919897No Hit
CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTT3070.1430828528949809No Hit
ATTTTTTCCATGAGCTTAATGATGCTTTCTCTGGGCTTTTGGGGGAGGGT3060.14261678497024158No Hit
GATCTGCCCGAGCTTTCAGCGTGTCATCGAAACCCTCCTCATGGACACAC3000.13982037742180545No Hit
AAGCATGGCAGCGGCAGAGGCTGGAGCAGTTGGGGATCTTCAGCTTCTAA3000.13982037742180545No Hit
GTATCAACGCAGAGTACATGGGATACTAGCCCACCAGACTCAGAGACGGA2990.1393543094970661No Hit
TCCCCCTCCTCCACCATGAAACTCGCTGTCACCCTCACCCTGGTCACACT2960.13795610572284805No Hit
GGCTCTCTGCTGCAGCTCCGCTTCTGCAGAGATCTGCCCGAGCTTTCAGC2940.13702396987336934No Hit
TTTCAGCCCTGATCAAGACATGAGGGAGGCAGGGGCTCAGCTGAAGAAGC2940.13702396987336934No Hit
CCCTCCAGTTATGAGGCTGCCATGGAACTTTTCAGCCCTGATCAAGACAT2910.13562576609915128No Hit
GCCTGGGGACCCAGCTCAGCCACACACTTCTGGGAGCCCTCTATGAGGTG2870.1337614944001939No Hit
GGGGAAGGGGATGGACGGTCCTCCCCGCGGCGGGGTTTTCAGCCCTCGCG2850.13282935855071518No Hit
GTCTTGATCAGGGCTGAAAAGTTCCATGGCAGCCTCATAACTGGAGGGTG2850.13282935855071518No Hit
GGCGTGGACTCAAAGCATGGCAGCGGCAGAGGCTGGAGCAGTTGGGGATC2820.13143115477649714No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC2780.12956688307753972No Hit
GGTTTATTGAAGAGAGCAAGGCTGGTGGGCGTGGACTCAAAGCATGGCAG2780.12956688307753972No Hit
GCCTGTGGCCCAGCCTGTCGCTGCGCTGGAGTCGGCGGCGGAGGCCGGGG2750.12816867930332168No Hit
CTCAGAGACGGAACCAGAGACGGGCCAGAGCATCCCCCTCCTCCACCATG2710.12630440760436426No Hit
CTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGG2700.1258383396796249No Hit
AGCTTTCAGCGTGTCATCGAAACCCTCCTCATGGACACACCCTCCAGTTA2690.12537227175488555No Hit
GGGGATGGACGGTCCTCCCCGCGGCGGGGTTTTCAGCCCTCGCGGGTGGG2640.1230419321311888No Hit
TGGACACACCCTCCAGTTATGAGGCTGCCATGGAACTTTTCAGCCCTGAT2600.12117766043223138No Hit
TCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCC2580.12024552458275269No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2580.12024552458275269No Hit
ACCAGAGACGGGCCAGAGCATCCCCCTCCTCCACCATGAAACTCGCTGTC2550.11884732080853462No Hit
CACCCTGGTCACACTGGCTCTCTGCTGCAGCTCCGCTTCTGCAGAGATCT2540.11838125288379528No Hit
ACCAGAGACAGGCCAGAGCATCCCCCTCCTCCACCATGAAACTCGCTGTC2530.11791518495905594No Hit
GTCTCGGCTCAGCCAAACACTGTCAGGGCCCCCAGCAGGGCCTTCAGGGC2510.11698304910957723No Hit
CAGTGAGCTTTGGGCTATTTTTTCCATGAGCTTAATGATGCTTTCTCTGG2500.11651698118483789No Hit
GTACATGGGGAGCCGCAGGTTCCCCGCGCGCCCCGAGCCCCCGCGCCATG2500.11651698118483789No Hit
TAATGATGCTTTCTCTGGGCTTTTGGGGGAGGGTGTCCACCAGCTTCTTC2480.11558484533535918No Hit
AATTACACAGTGAGCTTTGGGCTATTTTTTCCATGAGCTTAATGATGCTT2480.11558484533535918No Hit
CTCATGTCTTGATCAGGGCTGAAAAGTTCCATGGCAGCCTCATAACTGGA2480.11558484533535918No Hit
CTTGTGGTTTATTGAAGAGAGCAAGGCTGGTGGGCGTGGACTCAAAGCAT2440.11372057363640178No Hit
GTGAGCTTTGGGCTATTTTTTCCATGAGCTTAATGATGCTTTCTCTGGGC2350.1095259623137476No Hit
CTCTGCTGCAGCTCCGCTTCTGCAGAGATCTGCCCGAGCTTTCAGCGTGT2330.1085938264642689No Hit
CACTTCTGGGAGCCCTCTATGAGGTGGTTCACGGGGATGCCCAGGCTGCT2300.10719562269005085No Hit
GCATGGCAGCGGCAGAGGCTGGAGCAGTTGGGGATCTTCAGCTTCTAAAT2300.10719562269005085No Hit
CCCCAGCAGGGCCTTCAGGGCCTTCACGGCCCCCACGGCCTGGGGACCCA2290.10672955476531149No Hit
GTTTATTGAAGAGAGCAAGGCTGGTGGGCGTGGACTCAAAGCATGGCAGC2290.10672955476531149No Hit
CCCCCGCGCCATGAAGCTCGCCGCCCTCCTGGGGCTCTGCGTGGCCCTGT2280.10626348684057214No Hit
TCTATGAGGTGGTTCACGGGGATGCCCAGGCTGCTCAGCAGGAGCTTCAG2250.10486528306635409No Hit
GTGTAGATGCTCCAGTCTCGGCTCAGCCAAACACTGTCAGGGCCCCCAGC2250.10486528306635409No Hit
GCCCCCAGCAGGGCCTTCAGGGCCTTCACGGCCCCCACGGCCTGGGGACC2250.10486528306635409No Hit
CGGGTGGGCAGCGTCTTGTCCTCAGGTGTAGATGCTCCAGTCTCGGCTCA2240.10439921514161472No Hit
CCCTGATTCCCCGTCACCCGTGGTCACCATGGTAGGCACGGCGACTACCA2220.10346707929213604No Hit
TTTGTGATGCTTGTGGTTTATTGAAGAGAGCAAGGCTGGTGGGCGTGGAC2210.10300101136739667No Hit
GCCTGGGCATCCCCGTGAACCACCTCATAGAGGGCTCCCAGAAGTGTGTG2210.10300101136739667No Hit
CTCCTCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCG2190.10206887551791799No Hit
GCGCTGGAGTCGGCGGCGGAGGCCGGGGCCGGGACCCTGGCCAACCCCCT2190.10206887551791799No Hit
GAGCATCCCCCTCCTCCACCATGAAACTCGCTGTCACCCTCACCCTGGTC2180.10160280759317862No Hit
TATCTAGAGTCACCAAAGCCGCCGGCGCCCGCCCCCCGGCCGGGGCCGGA2180.10160280759317862No Hit
CTGCAGCTCCGCTTCTGCAGAGATCTGCCCGAGCTTTCAGCGTGTCATCG2170.10113673966843928No Hit
GATCAAGACATGAGGGAGGCAGGGGCTCAGCTGAAGAAGCTGGTGGACAC2160.10067067174369994No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)