FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003647634

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003647634
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54
Sequences flagged as poor quality0
Sequence length146
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA23.7037037037037033Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC23.7037037037037033Clontech Universal Primer Mix Long (96% over 26bp)
GGCCGAATGGGGCCGGAAAGAGATTGAGATTTCGGAGCACGAGATGCCCG23.7037037037037033No Hit
ACGCCAGGCAGGGCACCGTCGAGCACCTTCTTCACCTCATCGGCATTGAT23.7037037037037033No Hit
AGAGCCGCGTGGGCGAGGGCAGCACCTTCCGGCTGCAGCTGCCGCTGCAC23.7037037037037033No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT23.7037037037037033Clontech Universal Primer Mix Long (96% over 26bp)
GAGCCAATCCAGCTGGCCCGGGTCGAGGTAGCCGTGGGTGCGTCCCGGAA23.7037037037037033No Hit
GCAGTGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT11.8518518518518516Clontech Universal Primer Mix Long (100% over 23bp)
GGTATTGCCAAACGGTACTGGTAAAACTTCACGCGTTCTTGTTTTCGCAC11.8518518518518516No Hit
GCAGAGTACATGGAAAGCAGTGGTATCAACGCAGAGTACATGGAAGCCTG11.8518518518518516Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCTCTTAT11.8518518518518516Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTACTGGGGGTTTAAACC11.8518518518518516No Hit
GATGAGGCCGACCCCTCGGCCACGGGCAGGCAGGGTTCGGCCGACGCCGC11.8518518518518516No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT11.8518518518518516Clontech Universal Primer Mix Long (96% over 26bp)
GCTTGGTTTAGAGGCCTTACTCCTCGGTGGTTTCTTCTTCAGCGCTCTTC11.8518518518518516No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG11.8518518518518516Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAGTGTGCCTTTTTTAAA11.8518518518518516No Hit
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA11.8518518518518516No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTGGTTTTTACCACGGCTTACCA11.8518518518518516No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGCTGTCTCTTATACA11.8518518518518516Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CACACACACACACACACACACACACACACACACACCTGTCTCTTATACAC11.8518518518518516No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTGTGCCTGTTTTAA11.8518518518518516No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC11.8518518518518516Clontech Universal Primer Mix Long (96% over 26bp)
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA11.8518518518518516Clontech Universal Primer Mix Long (96% over 26bp)
GTGCAAATAATTTGCCAGAGCTGGGTAGTGTGGCCGAGCGGTCTAAGGCG11.8518518518518516No Hit
AATTTCACCTTATCAGCTATTTTAAAGTTTCTGAGAAAAATTCCTTTGGT11.8518518518518516No Hit
GGGCAGTGGTATCAACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11.8518518518518516No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTCTGT11.8518518518518516Clontech Universal Primer Mix Long (100% over 23bp)
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTACC11.8518518518518516Clontech Universal Primer Mix Long (96% over 26bp)
CGGCGTTGTCGGCGCCCCGGGCGCGGGCGCTCCCCGCGTTCTCGGCGCAC11.8518518518518516No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC11.8518518518518516Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG11.8518518518518516Clontech Universal Primer Mix Long (96% over 26bp)
CCGCGGCGGCCAGTGGATTGGCCGCGCCCATATTGGTGATGATGCGTATG11.8518518518518516No Hit
ACGCAGAGTACTTGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC11.8518518518518516Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATGACGGACAGCCTGGCTGACCGCGGTAACGACCGTCTTTTGCGCGTCGA11.8518518518518516No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA11.8518518518518516Clontech Universal Primer Mix Long (96% over 26bp)
GCGCATCGCGCAGGCTTTGCCACGGCTGTCTCGCTCGCACCGGCAGGTGG11.8518518518518516No Hit
ACGTGGAGTCGCCGTCCATCGACGTGCTCGAGGCGCTGATGGCACTGGGC11.8518518518518516No Hit
GGTGATGGACGGCCTGTTCACCCAGCCGCGCACCCGCATCGCCAGCCCCG11.8518518518518516No Hit
GTACATGGGAAGCAGTGGTGTCAACGCAGAGTACATGGGAAGCAGTGGTA11.8518518518518516Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GAACACCCCTATCTGAGACCCACCTCCATCTGTATTGACGCCACGTGAGC11.8518518518518516No Hit
GTTGATGAGGATCAGCGCCGGGTTGGCCAGGGGCAGCGGCTGGCCGTCGT11.8518518518518516No Hit
CTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGC11.8518518518518516Clontech Universal Primer Mix Long (96% over 25bp)
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA11.8518518518518516Clontech Universal Primer Mix Long (96% over 25bp)
GTGCCCACCGTCGCACTCAGGGAAACGGTCGGCAGCAGCGCGGCGCGTGC11.8518518518518516No Hit
CACACACACACACACACACACACACACACACACCTGTCTCTTATACACAT11.8518518518518516No Hit
GCGTCGAGCGGGCGGCCGGCGAAGCGGAATTCGCTGTCGTAGTCGTCCTC11.8518518518518516No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)