FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003647635

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003647635
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64
Sequences flagged as poor quality0
Sequence length146
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA69.375Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT34.6875Clontech Universal Primer Mix Long (96% over 26bp)
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA34.6875Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGT23.125Clontech Universal Primer Mix Long (96% over 26bp)
GAACCACAGTTCGCCCTGCTTGGTCACGTAGACCGAGCTCACCGCCCTGG23.125No Hit
GTACACCAGCGTGTTGATGCGCTCTTCCTCGTTCTTAAGGAAGGCCTTGG23.125No Hit
CCCTCTCCCGCTTGCGGGAGAGGGCGGGGGTGAGGGCAGGCGGCGTTGGA23.125No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG23.125Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATCTATGTATTAACCATGTTCTTACATCAAACTAATGTTCTTGTGTATC23.125No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC23.125Clontech Universal Primer Mix Long (96% over 26bp)
CACGTCGAGCGACTCGCCCTGCACAACGGCCGTACGGTTTTCCAGAGCAG23.125No Hit
CCGCGGGGGCACAGGACTTGGTGGCTGGTTGGTTAAGGCGATGGACTACA23.125No Hit
CAAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTTTAAACAC11.5625No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATCTGTCTCTTATACAC11.5625Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGTAAGCAGTGGTATCAACGCAGAGTACTT11.5625Clontech SMART CDS Primer II A (100% over 26bp)
GGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAGTACAATGC11.5625No Hit
ACGTTGTACTCGCGCATCAGCGAGGTCGTCACGATGGGCCAGCGCACCGA11.5625No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGTTTTTAACACTGGT11.5625No Hit
CAGTGGCAGCGTCTGGACGACGCCTACCGCGTCCGCGCGGGCCACGGCGG11.5625No Hit
GTATCAACGCAGAGTACATGGGAAACCAGGGGAAACAACGCAGAGTACTT11.5625No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTAT11.5625Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA11.5625No Hit
CTTCGGACTTCTTGCGGGTCGGTCCAAGGACCATGACCATGTTGCGGCCA11.5625No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCAAGTACTCTGCGTTGAT11.5625Clontech Universal Primer Mix Long (96% over 26bp)
CGATACCCCGTTCGGGCTGCCGATCCGTCCCTTGTCGGTGCCTGTCTCTT11.5625No Hit
CGCCCTGGCTGTCGCGCAGGGCCGCGACGCGCGCGGCGACGTTGTCGAGT11.5625No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTTGAAACAAAGGAT11.5625No Hit
CCGTCGACTGGACCGCGACCGGCGGAGGCCGCCGCTCGGCACCGGGGACC11.5625No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTACTGTCT11.5625Clontech Universal Primer Mix Long (96% over 26bp)
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG11.5625Clontech Universal Primer Mix Long (96% over 26bp)
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG11.5625No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTGGGAAGCAGTGGTA11.5625Clontech SMART CDS Primer II A (100% over 26bp)
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG11.5625Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCCCAGCACGCTGGCCTTGTCGCGGTCGATGTTCAGCTCCAGGGCGCGGC11.5625No Hit
GAGTACTTGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG11.5625Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTCAGCGCCGCCTGCATGCGCGGCATGCCCTCGTCGAGGTGGCGCATGA11.5625No Hit
GCGTGTTCCGCTTTCACCTGGAGCCGCAAGGCATCGAAGGCGCCGCTTCG11.5625No Hit
ATCGACAGCTGGCCCTGGTTGAGCGTGGGGTTCATGCTGCCGGCACCGAT11.5625No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTAAACGGGGT11.5625No Hit
CAACATCAAACCAACAAGACGCTCTTAAGATCTAATGAAAAAAAAAAAAA11.5625No Hit
ATCCAGAAGCGCTACGACTCGCCCGACGAGGCCGAGGCCGACATCCGCCG11.5625No Hit
GAGTACTGGGAAGCAGTGGTATCAACGCAGAGTACTGGGAAGCAGTGGTA11.5625Clontech SMART CDS Primer II A (100% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT11.5625Clontech Universal Primer Mix Long (96% over 26bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC11.5625Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGTGGCGACGGCAGCGAGAAAAAAAAAAAAAAAAAA11.5625No Hit
GGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAGTACTCTGC11.5625No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)