FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003647636

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003647636
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64
Sequences flagged as poor quality0
Sequence length146
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA34.6875Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC34.6875Clontech SMART CDS Primer II A (96% over 26bp)
CATGTGCTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC23.125Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG23.125Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCACGAAGGCTTGCAACTTTTCCGGCGGCAGCGGCAGCGGCAATTCGCTG23.125No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG23.125Clontech Universal Primer Mix Long (96% over 26bp)
GTGCAAATAATTTGCCAGAGCTGGGTAGTGTGGCCGAGCGGTCTAAGGCG23.125No Hit
GTGATGGACATCTCCGACCGCGTGGTGGTGCTGGACTACGGCAAGAAGAT23.125No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTGGGAAGCAGTGGTA23.125Clontech SMART CDS Primer II A (100% over 26bp)
GGCATCTAGCCTTGTTGTTTCTTGAGAATCATGTCGGGAGGCTGACGCTG23.125No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG23.125Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGT23.125Clontech Universal Primer Mix Long (96% over 26bp)
GGACGGCTGCGTGACCGACGCGGTGGTGGCCGAGAACCTCACGCAGGCAC23.125No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTCTGT23.125Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCTACACCTACGACAACAAGACCGAGACCAAGGACATCGGCACCGTC23.125No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGAAAAACAAA11.5625No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA11.5625Clontech SMART CDS Primer II A (96% over 26bp)
AGCTTCGACTGGCGCACGCTCACGCTGGTCGGCAAGCTCGCGCCGCAGAT11.5625No Hit
GGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAATTA11.5625Clontech Universal Primer Mix Long (95% over 22bp)
GGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAATT11.5625Clontech Universal Primer Mix Long (95% over 22bp)
ACGCAGAGTACTTTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTAGGAGGG11.5625No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA11.5625No Hit
CCGCTCGGCGATGTCGAACATCTTGGTCGAGCCCGCCTGCTGCGACAGCG11.5625No Hit
TTCCCCGGTGCCGAGCGGCGGCCTCCGCCGGTCGCGGTCCAGTCGACGGC11.5625No Hit
GCCTGGACTGGGTGCTGGGCCACCAGCGACTGACCCTGGTTGTGGCTGGC11.5625No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT11.5625Clontech Universal Primer Mix Long (96% over 26bp)
CCGCCATCAGCGCCGCGAACAACGCGATGTACGACAGGGAACGGGACGAA11.5625No Hit
GTACTTGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA11.5625Clontech Universal Primer Mix Long (96% over 26bp)
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCCTGTCT11.5625Clontech Universal Primer Mix Long (96% over 28bp)
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA11.5625No Hit
GGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAATAAAAAA11.5625Clontech Universal Primer Mix Long (95% over 22bp)
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTACC11.5625Clontech Universal Primer Mix Long (96% over 26bp)
CTACGGCACCAAGAAGGGCCACGTCGTGCGCTTCCTGAGCGCCGGCGACA11.5625No Hit
GGCTGGTGAGCGGTCTCTACGTATGCGGCTGGAGCAAGCGCGGGCCACAA11.5625No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA11.5625No Hit
AGCGCAGCCAGCGCGATGGCCTGGACGAGATCCTGCCGCGCCTGCAGAAG11.5625No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG11.5625Clontech Universal Primer Mix Long (96% over 26bp)
GATTACATTGGAAGCAGTGGTATCAACGCATAGTACATTTAAGAAGTGGT11.5625Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG11.5625No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCAAGTACTCTGCGT11.5625Clontech Universal Primer Mix Long (96% over 26bp)
GGTGGTGCTGACCATGGCGCTGTTCCTGCGCCGGCTGGCACCGACGCTGA11.5625No Hit
CGCCGGAGGTCGTGGCACCGCAGGGCGGATTCGTGACCTGGGTGTGGCGG11.5625No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGATTAC11.5625Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GCACCGACAAGGGACGGATCGGCAGCCCGAACGGGGTATCGCTGTCTCTT11.5625No Hit
AAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC11.5625Clontech Universal Primer Mix Long (96% over 26bp)
CCAGTACTCTGCGTTGATACCACTGCTTCCCAGTACTCTGCGTTGATACC11.5625Clontech Universal Primer Mix Long (96% over 26bp)
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCTCTTATACAC11.5625Clontech SMARTer II A Oligonucleotide (100% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)