FastQCFastQC Report
Tue 10 May 2022
EGAF00003647650

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003647650
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44016
Sequences flagged as poor quality0
Sequence length146
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACACACACACACACACACACACACACACACACACACACACACACACACA2660.6043256997455471No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT2380.5407124681933841No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT1000.22719011268629588No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA790.17948018902217378No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT720.16357688113413305No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC690.15676117775354417No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA650.14767357324609234No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA640.14540167211922936No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT610.13858596873864049No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA600.13631406761177753No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT590.13404216648491457No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC580.1317702653580516No Hit
CTCCTACACCTACCTCTCTCTGGGCTTCTATTTCGACCGCGATGATGTGG540.12268266085059978No Hit
TCTTAATAGTCTTGTCTCTGGATCCAGACAGCAAGAATGGCCCAGGTTTA540.12268266085059978No Hit
GCCTATACGGTGCCCTCGCTCTTGGGTCCGCGCGTCATCGGCAAAGTCTC530.12041075972373683No Hit
TCTCTATGTTGGTCAGGCTGGTCTCAAACTCCCGACCTCAGGTGATCTGC510.11586695747001091No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT510.11586695747001091No Hit
CCCTTGATCCGCCCCGCAGGCAGGTACTTCCCGGAGCGAGCGGGGAACGC510.11586695747001091No Hit
TCCTGTAACAACGCATCTCATATTTGGAATGACTATTAAAAAAACAACAA490.11132315521628498No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG480.10905125408942204No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG470.10677935296255905No Hit
ATCTACGGCCGCAGAGCGGCTGGCAGTTTCTTCGAGGACCTCAGCAAGAC470.10677935296255905No Hit
CTACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCG450.10223555070883315No Hit
ATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAAACTTCA450.10223555070883315No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)