FastQCFastQC Report
Tue 10 May 2022
EGAF00003647713

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003647713
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences363503
Sequences flagged as poor quality0
Sequence length146
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC20670.568633546353125No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC20360.5601054186622944No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT16040.441261832777171No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA15880.4368602184851294No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG15790.434384310445856No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA15410.42393047650225724No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT14850.40852482648011157No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC14000.38514125055364057No Hit
GTATCGGCTTCTATGGCGAATGACAGTGGAAAGCTGTGTGTTGATTTCAT13560.3730368112505261No Hit
GCAATATGCACTGCCGCGTCTGACGGGACAAGGGATCAACCTTTCCCGGC13090.3601070692676539No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC12400.3411251076332245No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA10570.2907816441679986No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT10420.28665513076920957No Hit
GCCCTAACTGCGCAGTTAATAATTCTGGCAATTCGTCTCCACACTAGAAG10180.2800527093311472No Hit
CCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTTGA9930.2731751869998322No Hit
ATTATTAACTGCGCAGTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTT9790.2693237744942958No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT9400.25859483965744434No Hit
AGCCGATACCGTGAAAGAAGAAAGAAAAACGGTGTGCTTCAAATTGCGCT9340.2569442342979288No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG9170.25226751911263456No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA9150.25171731732612934No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA9120.25089201464637156No Hit
ATCCTACTGTATCTGAAAGAAGAACACTGTAGCCGCTTGGCAGGACCATT9100.2503418128598664No Hit
CTTCATAGCTGTCAGCACTCGTCAGGCGGTTGAACCATGTTGATTTCCCT8890.24456469410156176No Hit
ATATAGATTGGCGCTAGTAGATCACAGAACAACCGCCGCATACGGCCGAT8190.22530763157387973No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT7950.2187052101358173No Hit
CAGTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTA7800.2145786967370283No Hit
GTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTTCGCCTTGACCGCGGG7700.2118276878045023No Hit
GCCTAGATGTGATACACGTTTCTGGAAACTGCCTCGTCATGCGACTGTTC7660.2107272842314919No Hit
ATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAAACTTCA7560.2079762752989659No Hit
CCCTTAGACGTCTTGGTATACGGACAACTGATGGACCCACGTTGCGAGTC7540.2074260735124607No Hit
GTGGAAAGCTGTGTGTTGATTTCATGAATGCGATTTCTGATATGGCGGCG7150.19669713867560928No Hit
GTTTTTTCATGTCCCGCATAATCCTCATTTGAAGAATCAATTAAATGCAG6960.19147022170380987No Hit
ATCCAAGATAGTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTG6820.18761880919827345No Hit
GCTGTAAAGAGGGGCGTTGAGTCCGTCCGACTTCACTGCCCCCTTTCAGC6720.18486780026574745No Hit
GCTACATATATAGGAAAAGGGAAGGTAGAAGAGCTGAAGGCACTCGTGGA6590.18129148865346367No Hit
ATTTGAAGCACACCGTTTTTCTTTCTTCTTTCACGGTATCGGCTTCTATG6560.18046618597370587No Hit
ATATATAGGAAAAGGGAAGGTAGAAGAGCTGAAGGCACTCGTGGAAGAGC6520.17936578240069545No Hit
CCCCTATCCCCTACGAGGCTACCCGGATCGATGACGCGAATTGGGGACAT6510.17909068150744287No Hit
GAATAAATCCTACTGTATCTGAAAGAAGAACACTGTAGCCGCTTGGCAGG6260.17221315917612784No Hit
GTCTTCTTCATAGCTGTCAGCACTCGTCAGGCGGTTGAACCATGTTGATT6260.17221315917612784No Hit
GTACATGGGGGAATTCGAGCTCGCATTTTGAAAATTCTATGGAAGAGCTA6210.17083765470986484No Hit
AGGCTACTACGTGCGCGATATGACAAGTTAACAAGACGGCGCAGGTTGAT6130.16863684756384403No Hit
CTACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCG6110.16808664577733884No Hit
GAATTAAATCCGCTTCTTTTACTTCCTCAAGCGTTGAGCGGAATGCAGCA6090.16753644399083364No Hit
GTAATATAGATTGGCGCTAGTAGATCACAGAACAACCGCCGCATACGGCC6040.16616093952457062No Hit
ACATATATAGGAAAAGGGAAGGTAGAAGAGCTGAAGGCACTCGTGGAAGA5960.16396013237854984No Hit
ATCCTGAATAAATCCTACTGTATCTGAAAGAAGAACACTGTAGCCGCTTG5890.16203442612578162No Hit
GTCTCAGGGGATCTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGC5860.16120912344602384No Hit
ATACAAACGCAGGGAAATCAACATGGTTCAACCGCCTGACGAGTGCTGAC5840.16065892165951862No Hit
GAACTCGGATAGGTCTCAGAAAAACGAAATATAGGCTTACGGTAGGTCCG5820.16010871987301342No Hit
ACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCGAT5690.15653240826072964No Hit
GCATAATCCTCATTTGAAGAATCAATTAAATGCAGAATTAAATCCGCTTC5690.15653240826072964No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG5650.1554320046877192No Hit
ATGCACTGCCGCGTCTGACGGGACAAGGGATCAACCTTTCCCGGCAAGGC5640.15515690379446662No Hit
TCTTTAAAGGCTGCAGCGTCGTCCTCAAATTTCGCACTGACCATAATGTG5590.15378139932820362No Hit
TTCCAGAGCTGTACGGTCGCACTGAACTCGGATAGGTCTCAGAAAAACGA5510.1515805921821828No Hit
GCTTTCCACTGTCATTCGCCATAGAAGCCGATACCGTGAAAGAAGAAAGA5490.15103039039567762No Hit
GAATGCAGCAATCAATGTCGTCGGAAGATCCTGAATAAATCCTACTGTAT5470.15048018860917242No Hit
GCGTTTGTATAGCCGACAAGCGCAATTTGAAGCACACCGTTTTTCTTTCT5400.14855448235640423No Hit
GTATCTGAAAGAAGAACACTGTAGCCGCTTGGCAGGACCATTTTTCTGGT5330.146628776103636No Hit
TCTTTAATGATGAACTGTCGCCAAGTCAGCTGAAGTCATTGGCAACAGCA5270.1449781707441204No Hit
CTGTAGCCGCTTGGCAGGACCATTTTTCTGGTCATCGGGTCCAGCGTGGC5250.1444279689576152No Hit
GGACAAGGGATCAACCTTTCCCGGCAAGGCGGAGGAATTGGGGCAAGAGG5190.1427773635980996No Hit
GAAAAGTACTGACCAGCGTCACACAAAAACGGAACAGGGCTGACGCCGCT5130.141126758238584No Hit
GGTGTACACCGCCCTTAGACGTCTTGGTATACGGACAACTGATGGACCCA5090.1400263546655736No Hit
ATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAATTT5090.1400263546655736No Hit
GAAGATGATTGACCGCACGCAATTGATATTAGATATTTTTGCAAAGCGGG5020.1381006484128054No Hit
GCTGCAATATGCACTGCCGCGTCTGACGGGACAAGGGATCAACCTTTCCC5020.1381006484128054No Hit
ATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTCAAGCA4930.135624740373532No Hit
GGCTAGACTAGCGTACGAGCACTATGGTCAGTAATTCCTGGAGGAATAGG4860.13369903412076378No Hit
CTGCCCTAACTGCGCAGTTAATAATTCTGGCAATTCGTCTCCACACTAGA4830.13287373144100598No Hit
CACTAGAAGTCGACGAACAACGAAGAGCGACGATGCCCGTTTCAGGTGGT4720.1298476216152274No Hit
CAGTAGGATTTATTCAGGATCTTCCGACGACATTGATTGCTGCATTCCGC4720.1298476216152274No Hit
GAGTAATATAGATTGGCGCTAGTAGATCACAGAACAACCGCCGCATACGG4710.12957252072197478No Hit
TCATAGCTGTCAGCACTCGTCAGGCGGTTGAACCATGTTGATTTCCCTGC4710.12957252072197478No Hit
GCTTCAAATTGCGCTTGTCGGCTATACAAACGCAGGGAAATCAACATGGT4690.1290223189354696No Hit
ATCCTCATTTGAAGAATCAATTAAATGCAGAATTAAATCCGCTTCTTTTA4670.1284721171489644No Hit
ATGTTGATTTCCCTGCGTTTGTATAGCCGACAAGCGCAATTTGAAGCACA4660.12819701625571178No Hit
GTAAAGAGGGGCGTTGAGTCCGTCCGACTTCACTGCCCCCTTTCAGCCTT4640.1276468144692066No Hit
GTTGATTTCCCTGCGTTTGTATAGCCGACAAGCGCAATTTGAAGCACACC4570.1257211082164384No Hit
CCATAGAAGCCGATACCGTGAAAGAAGAAAGAAAAACGGTGTGCTTCAAA4560.12544600732318578No Hit
GTGCTAGCCCGTCGAGACTGAAAAGCTATAACCCGCAGACCCGAGCGAAA4540.12489580553668059No Hit
ATGATGAACTGTCGCCAAGTCAGCTGAAGTCATTGGCAACAGCAATTGAA4520.12434560375017539No Hit
GGATATCATCTGCTTCAAGCTCCTCAAGCAGCCGAAGCACTGTTTTTTCA4500.12379540196367018No Hit
GATTATAACAACGGTGCAATCTCAGCTAAACCGACGCAGTTTTGCTCCTT4500.12379540196367018No Hit
GCCGTACGCTGAGGACCACCTGAAACGGGCATCGTCGCTCTTCGTTGTTC4480.12324520017716496No Hit
CTTCAAATTGAGCTGGCTCAGCTGCAATATGCACTGCCGCGTCTGACGGG4470.12297009928391237No Hit
GTACTGACCAGCGTCACACAAAAACGGAACAGGGCTGACGCCGCTACATA4440.12214479660415456No Hit
GTATGTCCCAAGGGGACTTAAGCGCGGTGGCCTCCCCTATCCCCTACGAG4390.12076929213789156No Hit
TCATGCGACTGTTCCCCGGGGTCAGGGCCGCTGGTATTTGCTGTAAAGAG4380.12049419124463896No Hit
GTCATTCGCCATAGAAGCCGATACCGTGAAAGAAGAAAGAAAAACGGTGT4380.12049419124463896No Hit
CTCGTGGAAGAGCTTGAAGCTGATCTCCTCATCTTTAATGATGAACTGTC4370.12021909035138637No Hit
ATTAAATGCAGAATTAAATCCGCTTCTTTTACTTCCTCAAGCGTTGAGCG4350.11966888856488117No Hit
GGGCTGACGCCGCTACATATATAGGAAAAGGGAAGGTAGAAGAGCTGAAG4340.11939378767162857No Hit
GCACTATGGTCAGTAATTCCTGGAGGAATAGGTACCAAGAAAAAAACGAA4330.11911868677837596No Hit
GCTATGAAGAAGACCTCCTGTTTGCCACGCTGGACCCGATGACCAGAAAA4310.11856848499187077No Hit
GGTATTTGCTGTAAAGAGGGGCGTTGAGTCCGTCCGACTTCACTGCCCCC4290.11801828320536557No Hit
AGGTAGAAGAGCTGAAGGCACTCGTGGAAGAGCTTGAAGCTGATCTCCTC4280.11774318231211296No Hit
GGCTTACGGTAGGTCCGAATGGCACAAAGCTTGTTCCGTTAGCTGGCATA4250.11691787963235517No Hit
ATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAAAC4170.11471707248633436No Hit
GCGTACGGCGGGACCTCTGAGAATTGGGATACAGGACCCAAAAGGCTGAA4150.11416687069982917No Hit
ATTCAAATGAGCATCCTAGTCACCGCGTTTAAAATGAACCTGCCGGCTGA4150.11416687069982917No Hit
CCTCAAGCAGCCGAAGCACTGTTTTTTCATGTCCCGCATAATCCTCATTT4150.11416687069982917No Hit
CATTTGAAGAATCAATTAAATGCAGAATTAAATCCGCTTCTTTTACTTCC4140.11389176980657657No Hit
CTTTACAGCAAATACCAGCGGCCCTGACCCCGGGGAACAGTCGCATGACG4120.11334156802007136No Hit
GCTTGAAGCTGATCTCCTCATCTTTAATGATGAACTGTCGCCAAGTCAGC4100.11279136623356614No Hit
GCATATTGCAGCTGAGCCAGCTCAATTTGAAGTTTGCCTTCTCTCGTTCT4080.11224116444706095No Hit
GTCGCCAAGTCAGCTGAAGTCATTGGCAACAGCAATTGAAGTGAAGATGA4070.11196606355380835No Hit
CGTTAACAGTTCTTGGCGCAAATAGCGCTGAATCGCTTCTTTAAAGGCTG4010.11031545819429275No Hit
GTCCCAAGGGGACTTAAGCGCGGTGGCCTCCCCTATCCCCTACGAGGCTA3970.10921505462128235No Hit
GTGATACACGTTTCTGGAAACTGCCTCGTCATGCGACTGTTCCCCGGGGT3960.10893995372802975No Hit
CTCCAGATTACTTCCATTTCCGCCCAAGCTGCTCACAGTATACGGGCGTC3960.10893995372802975No Hit
GATGATTGACCGCACGCAATTGATATTAGATATTTTTGCAAAGCGGGCGA3950.10866485283477716No Hit
CCGCTACATATATAGGAAAAGGGAAGGTAGAAGAGCTGAAGGCACTCGTG3940.10838975194152456No Hit
CAATTGAAGTGAAGATGATTGACCGCACGCAATTGATATTAGATATTTTT3930.10811465104827195No Hit
GTAGAAGAGCTGAAGGCACTCGTGGAAGAGCTTGAAGCTGATCTCCTCAT3880.10673914658200895No Hit
GTATAGCCGACAAGCGCAATTTGAAGCACACCGTTTTTCTTTCTTCTTTC3840.10563874300899856No Hit
CATCTAGGCATGGAATCTTATGCCAGCTAACGGAACAAGCTTTGTGCCAT3840.10563874300899856No Hit
CTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGCTTCCTTCCGCAG3840.10563874300899856No Hit
GTTTTTGATCACGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCT3790.10426323854273556No Hit
GTGTTGATTTCATGAATGCGATTTCTGATATGGCGGCGGTCGGTTTCCAG3770.10371303675623035No Hit
ATTCAGGATCTTCCGACGACATTGATTGCTGCATTCCGCTCAACGCTTGA3760.10343793586297775No Hit
GCTCACAGTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTT3730.10261263318321996No Hit
TGACTGGACACTGCATCGGAAGACACTTCGTGCCGACACGAAGCAGCCTC3730.10261263318321996No Hit
ATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTTGATCACG3710.10206243139671474No Hit
GAACGAGAGAAGGCAAACTTCAAATTGAGCTGGCTCAGCTGCAATATGCA3710.10206243139671474No Hit
GAATTGGGGCAAGAGGTCCCGGGGAAACGAAACTGGAAACCGACCGCCGC3710.10206243139671474No Hit
ACCTTTCCCGGCAAGGCGGAGGAATTGGGGCAAGAGGTCCCGGGGAAACG3680.10123712871695693No Hit
CGGTTGAACCATGTTGATTTCCCTGCGTTTGTATAGCCGACAAGCGCAAT3670.10096202782370434No Hit
ACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTTG3660.10068692693045174No Hit
GTCCAGTCATGACCTCGATCCATCGCGTATAGGGACGCCCCCTGCTCGCG3650.10041182603719914No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)