FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003647715

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003647715
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4430
Sequences flagged as poor quality0
Sequence length146
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTCAGGGTTGTACATAAAAAATCCAGGTTTGTGGAAGTCGCGTTCTTTA140.3160270880361174No Hit
GTTTTATTATCATTCACATTATTTCATAGAAAAAGGAATATAGCAAACGG120.27088036117381487No Hit
CCTCTATATAAATGCGTAGGGGTTTTAGTTAAATGTCCTTTGAAGTATAC80.1805869074492099No Hit
GTCCCGACTCTAGCCGCCTCTTCAGCTCGCCATGGATCCCAACTGCTCCT80.1805869074492099No Hit
CTCTAGAGGAGCCTGTTCTGTAATCGATAAACCCCGATCAACCTTACCAC80.1805869074492099No Hit
CCCCAGGTATATTTGGGATAGTTGGCTCCTCGCTGGGTCAGGATGGCTGG70.1580135440180587No Hit
CTCCAAGTCCCAGCGAACCCGCGTGCAACCTGTCCCGACTCTAGCCGCCT70.1580135440180587No Hit
CTATATAAATGCGTAGGGGTTTTAGTTAAATGTCCTTTGAAGTATACTTG70.1580135440180587No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70.1580135440180587No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC60.13544018058690743No Hit
ATACAGGACTCGGCTCCGGGGGCAGGGCGAGGAATGGAAGTTGGAGTACG60.13544018058690743No Hit
GTACATGGGAACACATCCAAGCTTAAGACGGTGAGGTCAGCTTCACATTC60.13544018058690743No Hit
TAGTAGAACCTCGCAATGACAGCCGCAATGGCAGACCCAATGGAGCCCAG60.13544018058690743No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG60.13544018058690743No Hit
TATCAACGCAGAGTACATGGGAACACATCCAAGCTTAAGACGGTGAGGTC60.13544018058690743No Hit
GATTGCTACAGTTGTGATTGGAGGAGTTGTGGCTGTGCCCATGGTGCTCA50.1128668171557562No Hit
AGAAAATGTAGCCCATTTCTTGCCACCTCATGGGCTACACCTTGACCTAA50.1128668171557562No Hit
GCCATGGAGAAGGGCTAATAGAAGCAGGTCCCTTGCCCAGACCCTCAGGG50.1128668171557562No Hit
GGTATATTTGGGATAGTTGGCTCCTCGCTGGGTCAGGATGGCTGGGTGCC50.1128668171557562No Hit
TATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATG50.1128668171557562No Hit
ATCCATAGGCCTGGAAGATCAGCACTGGGATGACGATGAGCAGAATGGTC50.1128668171557562No Hit
TTACTAGAAGAGGAATTTCTCAAAGTATACCTGTGTTCTTGCGGGGCGTG50.1128668171557562No Hit
CATTATTTCATAGAAAAAGGAATATAGCAAACGGTCAGGGTCAGGGTTGT50.1128668171557562No Hit
GGAATATAGCAAACGGTCAGGGTCAGGGTTGTACATAAAAAATCCAGGTT50.1128668171557562No Hit
GTTGTACATAAAAAATCCAGGTTTGTGGAAGTCGCGTTCTTTACATCTGG50.1128668171557562No Hit
GTACATGGGGAACACATCCAAGCTTAAGACGGTGAGGTCAGCTTCACATT50.1128668171557562No Hit
GTCCCAGCGAACCCGCGTGCAACCTGTCCCGACTCTAGCCGCCTCTTCAG50.1128668171557562No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA50.1128668171557562No Hit
ATATAAATGCGTAGGGGTTTTAGTTAAATGTCCTTTGAAGTATACTTGAG50.1128668171557562No Hit
CTCCTGATCTATCCATAGGCCTGGAAGATCAGCACTGGGATGACGATGAG50.1128668171557562No Hit
GCCTAGCAGTCGCTCCATTTATCGCTTGAGATCTCCAGCCTTACCGCGGC50.1128668171557562No Hit
GCTGTGCTGGGGGCGCCCCACAACCCTGCTCCCCCGACGTCCACCGTGAT50.1128668171557562No Hit
GTATCAACGCAGAGTACATGGGGAACACATCCAAGCTTAAGACGGTGAGG50.1128668171557562No Hit
CTGTAGACAGGTGTGTGGGTATAAACTGCTGTATCTAGGGGCAGGACCAA50.1128668171557562No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)