FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003647717

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003647717
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4506
Sequences flagged as poor quality0
Sequence length146
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGTAAG120.2663115845539281No Hit
CATTATTTCATAGAAAAAGGAATATAGCAAACGGTCAGGGTCAGGGTTGT100.22192632046160676No Hit
GATCCCAACTGCTCCTGCGCCGCCGGTGACTCCTGCACCTGCGCCGGCTC90.1997336884154461No Hit
GGGTTGTACATAAAAAATCCAGGTTTGTGGAAGTCGCGTTCTTTACATCT90.1997336884154461No Hit
GTCACGGTCAGGGTTGTACATAAAAAATCCAGGTTTGTGGAAGTCGCGTT90.1997336884154461No Hit
GGTCAGGGTTGTACATAAAAAATCCAGGTTTGTGGAAGTCGCGTTCTTTA80.17754105636928538No Hit
GTACATGGGACCACGCCTCCTCCAAGTCCCAGCGAACCCGCGTGCAACCT80.17754105636928538No Hit
ATTTGGTATATTTCACCCCAGGTATATTTGGGATAGTTGGCTCCTCGCTG80.17754105636928538No Hit
CTCCAAGTCCCAGCGAACCCGCGTGCAACCTGTCCCGACTCTAGCCGCCT80.17754105636928538No Hit
TATCAACGCAGAGTACATGGGACCACGCCTCCTCCAAGTCCCAGCGAACC80.17754105636928538No Hit
GTTTTATTATCATTCACATTATTTCATAGAAAAAGGAATATAGCAAACGG70.1553484243231247No Hit
GTCCCAGCATCAGGCGCAGCAGCTGCACTTGTCCGACGCCCCTTTGCAGA70.1553484243231247No Hit
GAACACATCCAAGCTTAAGACGGTGAGGTCAGCTTCACATTCTCAGGAAC70.1553484243231247No Hit
GTTCTCTTCTCTGCAGGGCGAGGGGCAGGGAGCTAGTAGAACCTCGCAAT70.1553484243231247No Hit
CACCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAGACGTT60.13315579227696406No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT60.13315579227696406No Hit
ACCCTGCTCCCCCGACGTCCACCGTGATCCACATCCGCAGCGAGACCTCC60.13315579227696406No Hit
GTATCAACGCAGAGTACATGGGAACACATCCAAGCTTAAGACGGTGAGGT60.13315579227696406No Hit
CCTCCTCCAAGTCCCAGCGAACCCGCGTGCAACCTGTCCCGACTCTAGCC60.13315579227696406No Hit
GAGTAGCCACAAGGCTGCCCGAGGCAACTCCACCCCCATTGGCAATGGCC60.13315579227696406No Hit
TCTCTGCAGGGCGAGGGGCAGGGAGCTAGTAGAACCTCGCAATGACAGCC60.13315579227696406No Hit
ATATAAATGCGTAGGGGTTTTAGTTAAATGTCCTTTGAAGTATACTTGAG60.13315579227696406No Hit
GTACATGGGAACACATCCAAGCTTAAGACGGTGAGGTCAGCTTCACATTC60.13315579227696406No Hit
CTCTAGAGGAGCCTGTTCTGTAATCGATAAACCCCGATCAACCTTACCAC60.13315579227696406No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60.13315579227696406No Hit
GGGTCAGGGTTGTACATAAAAAATCCAGGTTTGTGGAAGTCGCGTTCTTT60.13315579227696406No Hit
ATCCGGAGAGTCCAGTTGCTCCCAGTGACTGCAGAGTAGCCACAAGGCTG50.11096316023080338No Hit
AAATAGACTACGAAAGTGGCTTTAACATATCTGAACACACAATAGCTAAG50.11096316023080338No Hit
GCCCCACAGCCTAGACCTCAGCCACAACTCGCTGCGCGCCACCGTAAACC50.11096316023080338No Hit
ACGCAGAGTACATGGGACGCCTCCTCCAAGTCCCAGCGAACCCGCGTGCA50.11096316023080338No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA50.11096316023080338Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AATGCAAAGAGTGCAAATGCACCTCCTGCAAGAAAAGCTGCTGCTCCTGC50.11096316023080338No Hit
CTTCAGCTCGCCATGGATCCCAACTGCTCCTGCGCCGCCGGTGACTCCTG50.11096316023080338No Hit
ATACAGGACTCGGCTCCGGGGGCAGGGCGAGGAATGGAAGTTGGAGTACG50.11096316023080338No Hit
GTCCCTGTTCAACACCCTCTTCTTGAACTGGTGCTGTCTGGGCTTCATAG50.11096316023080338No Hit
TATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATG50.11096316023080338No Hit
CGTGCAACCTGTCCCGACTCTAGCCGCCTCTTCAGCTCGCCATGGATCCC50.11096316023080338No Hit
CCACTGGATTTGACTCAGAGAGGACCCCCAGAGGGTGTCTCCATCTTCCC50.11096316023080338No Hit
GTACATGGGCAACTGTGCAGACTGCCCCCAGTCTAGGCACCTGTAACTCT50.11096316023080338No Hit
GGTTCACACGGCGCAGGGGGGACACATCCACAGCCTGTCGTCTCACAGTC50.11096316023080338No Hit
CTTTATTGAATGCCATTGTAGAAAAGCGTGTGAGGATAAAGGGCTGATAC50.11096316023080338No Hit
ATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCGAATGGCTCATTAA50.11096316023080338No Hit
ACTCTAGCCGCCTCTTCAGCTCGCCATGGATCCCAACTGCTCCTGCGCCG50.11096316023080338No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)