FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003647718

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003647718
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4506
Sequences flagged as poor quality0
Sequence length146
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAGAGGAGCCTGTTCTGTAATCGATAAACCCCGATCAACCTTACCAC130.28850421660008874No Hit
GGGTCAGGGTTGTACATAAAAAATCCAGGTTTGTGGAAGTCGCGTTCTTT110.24411895250776744No Hit
CTCCAAGTCCCAGCGAACCCGCGTGCAACCTGTCCCGACTCTAGCCGCCT100.22192632046160676No Hit
GTCCCAGCATCAGGCGCAGCAGCTGCACTTGTCCGACGCCCCTTTGCAGA90.1997336884154461No Hit
GGCTACACCTTGACCTAACGTCTTTACGTGGGTACTTGCGCTTACTTTGT80.17754105636928538No Hit
CTTTACATCTGGGAGCGGGGCTGTCCCAGCATCAGGCGCAGCAGCTGCAC80.17754105636928538No Hit
GGCATGGAGGCCTCTGCTCTCACCTCATCAGCAGTGACCAGTGTGGCCAA70.1553484243231247No Hit
ATGCAGCCCTGGGCACACTTGGCACAGCCCACAGGGCAGCAGGAGCAGCA70.1553484243231247No Hit
GGTATATTTGGGATAGTTGGCTCCTCGCTGGGTCAGGATGGCTGGGTGCC70.1553484243231247No Hit
GTCCAAACCTTCTTCTCTCCTGTCAACAGTGGCCAGCCCCCCAACTATGA70.1553484243231247No Hit
ATATAAATGCGTAGGGGTTTTAGTTAAATGTCCTTTGAAGTATACTTGAG70.1553484243231247No Hit
CTATATAAATGCGTAGGGGTTTTAGTTAAATGTCCTTTGAAGTATACTTG70.1553484243231247No Hit
GTTTTATTATCATTCACATTATTTCATAGAAAAAGGAATATAGCAAACGG60.13315579227696406No Hit
GTACATGGGACAGTGGTGTCCGAGAAGTCAGGCACGTAGCTCAGCGGCGG60.13315579227696406No Hit
GTGCCATGGGCTTCACTGCGGCGGGAATCGCCTCGTCCTCCATAGCAGCC60.13315579227696406No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60.13315579227696406No Hit
CTCCTGCACCTGCGCCGGCTCCTGCAAATGCAAAGAGTGCAAATGCACCT60.13315579227696406No Hit
GTCACGGTCAGGGTTGTACATAAAAAATCCAGGTTTGTGGAAGTCGCGTT60.13315579227696406No Hit
GTGCGGGGGCGCACCCTCTCTTCGCCTTCCTGCGGGAGGCCCTGCCAGCT60.13315579227696406No Hit
GCGTGCAACCTGTCCCGACTCTAGCCGCCTCTTCAGCTCGCCATGGATCC50.11096316023080338No Hit
CTTCAGGGCCCTGTTCAACTAAGCACTCTACTCTCAGTTTACTGCTAAAT50.11096316023080338No Hit
GTACATGGGACGCCTCCTCCAAGTCCCAGCGAACCCGCGTGCAACCTGTC50.11096316023080338No Hit
GCTCCACACTGTGCCCGTAGTCCCACACGCAGATCACGTCGCCCGAGAAC50.11096316023080338No Hit
CCAGTACATCGCGGTGCACGTGGTCCCGGACCAGCTCATGGCCTTCGGCG50.11096316023080338No Hit
GCTTTTATTTTACTTCAAAGTTTACCTCAGATCAGCCTGGGAAGGTGAGG50.11096316023080338No Hit
CGCCTCCTCCAAGTCCCAGCGAACCCGCGTGCAACCTGTCCCGACTCTAG50.11096316023080338No Hit
AATCCGGAGAGTCCAGTTGCTCCCAGTGACTGCAGAGTAGCCACAAGGCT50.11096316023080338No Hit
GTACATGGGAACACATCCAAGCTTAAGACGGTGAGGTCAGCTTCACATTC50.11096316023080338No Hit
GGTATAAACTGCTGTATCTAGGGGCAGGACCAAGGGGGCAGGGGCAACAG50.11096316023080338No Hit
CAATAGCTAAGACCCAAACTGGGATTAGATACCCCACTATGCTTAGCCCT50.11096316023080338No Hit
CTCCTGATCTATCCATAGGCCTGGAAGATCAGCACTGGGATGACGATGAG50.11096316023080338No Hit
GCATCAGGCGCAGCAGCTGCACTTGTCCGACGCCCCTTTGCAGATGCAGC50.11096316023080338No Hit
CACTATGAGTGCCGTGCTCCAGGTGGCCACTGGCGTCATCTCTACGCTGC50.11096316023080338No Hit
GGGTGGCCTCTGGCTCTGCCGTAGTTTTGCCCCTGGCCAGGATTGCTACA50.11096316023080338No Hit
GCCTATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGTAAG50.11096316023080338No Hit
GGCTGGGACCACGCCGGAGTTCATTGAGCCCTCGTGGGGGAGGGCAGTTC50.11096316023080338No Hit
CCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTGATTCCCCGTCACCC50.11096316023080338No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)