FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003647883

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003647883
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81
Sequences flagged as poor quality0
Sequence length146
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACATCCTCGGCCCGGACGGTCATGGCGACCACCACGAGGAGAAGGCCG44.938271604938271No Hit
TTCTTCGCCAGGCCAGGAATCACAAGCTCCGGGTGGATAAGGCAGCTGCT33.7037037037037033No Hit
CGGTGAGCACGGCGAGCGCGCCGGCCAGCGACTCGAGAACGAGCTCGCGC22.4691358024691357No Hit
AAGGCTTAGGAAACAGAGACACGTTTGGTACAGTGCAAGCAGATATTTGA22.4691358024691357No Hit
TGGAGAGACAGACGGTCAAGGGTTCCGGGGGCAAGGCAACAGTCTCTTCA22.4691358024691357No Hit
GTACGAATACAGACCGTGAAAGCGGGGCCTCACGATCCTTCTGACCTTTT22.4691358024691357No Hit
TCTTTGCTGCTAGGACTGGACATGGATGGCCAGCAGTAGACTACATGGAC22.4691358024691357No Hit
CGGAAGAAGCGCTGCAGGCCTCGCTCGCACGCCACGGCCACACCGGCGCA22.4691358024691357No Hit
GGCGCAGCCGGGGGTGTACGAGCAGAGCATCGCCCCCCACCTCACGCCGG22.4691358024691357No Hit
CTGTAGACCAACATCCAGTGTGATTTTGTGTCTGAGACCACACCCCAGTA22.4691358024691357No Hit
CACTTGGAAGCTCTGTAATTTCAATGATGACTGTGCTTTTAATCCGTTTT22.4691358024691357No Hit
GCGCCAGCTCCACGAAGGCGTCGAGGCGGCGCAACGGGATGTCGGTCTGG22.4691358024691357No Hit
GGCGCAGTGGCTCATGCCTGAAATCCCAGCACTTTGGGAGGCTGAGGTAG22.4691358024691357No Hit
GGCGTGAACACCGCGATCCTGTCGCCCACCGGCGGCTCGATCGGCATCGG22.4691358024691357No Hit
CTCCCCGAAGGTGAAAGGCACGAGAGCCGGGCGCGTGTCCGTGACCTTGA22.4691358024691357No Hit
GGATAAAACTGCGTGGCGGGGGTGCGTCGGGTCTGCGAGAGCGCCAGCTA22.4691358024691357No Hit
GCGCCAAGGCGTCTGCTGCCGGAAAGATCCATTCCAACTCCTGCCCCACC11.2345679012345678No Hit
CAGTTACGCTGACGTGTTAGAACTTGAGAAATATACCGCGTTCGTGGATT11.2345679012345678No Hit
GATGCCGGCGCGGGCCTCGATGTCGCGGCGCATCGCCTCGGTCCATGGCT11.2345679012345678No Hit
TCCCGACTGCATGGGCCGCCCCGTGCGCGAGGTCTGGCCGGACCTCGCAG11.2345679012345678No Hit
GAATGGAGACAATATAATCTCCTTCCCGGGCTCCTGCCACCTGAAAAAGT11.2345679012345678No Hit
GGCAGAGGCGGCGGACGCGGACGTCGTGGTCAAGGCGTCGGGCGTCGGCG11.2345679012345678No Hit
GGAATGGAATGGTACGGAATAGAATGGAATGGAAAGAATTGGAATGGAAT11.2345679012345678No Hit
GTTGTAATTCAACTTTCGAGTTTCTTATGGGGTTGATGTGACGTACGGTT11.2345679012345678No Hit
CCAGGAACTCGTTCTTGCGCTCGTCCGCCACGCGCAGCGCCTGCAGCGCC11.2345679012345678No Hit
CCCGGGAGTGGTCCCTGGGTAAGTGCAGGTAGGAGGGGGTGCTGTCTCTT11.2345679012345678No Hit
GCCTCATTCCTGGCTGATTGGCCGCGAAACCCTGGGCCCGCTGGAACGGG11.2345679012345678No Hit
GTGGTATGTGCCTGTAGTCCCAGCTTCTCAGGAGGCTGAGGCTGGTGGAG11.2345679012345678No Hit
CCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTGCCTCATATTCT11.2345679012345678Illumina Single End PCR Primer 1 (97% over 40bp)
TACCTGAATGCAATGGAATGGAACGGAGTGGAAAGGAATGGAATGGAATG11.2345679012345678No Hit
CTTTTTCTGGATCTGATACACCAATTTCTATGTCAAAAATGTCTCCATTT11.2345679012345678No Hit
GTGAAGGAGGAGCCCATGCCCTTGTTGGCCCGGGCGATGCACACGCCCTC11.2345679012345678No Hit
GAACGAGCGGCTGCCGAGCTGCTGGAACGCGTCGAGGCCGAGCACCGAGC11.2345679012345678No Hit
CATTTCAACTCTGAACTTGAAATAGAGCTCAGAACGGACGCAAGTGAAGT11.2345679012345678No Hit
CTCAGATATGGTGCCGACAGGCTTCCACGGTGTCGAGCTAGCAGACGTCC11.2345679012345678No Hit
GTGCCAAATCCTCAACGCATTTCTTTGCAACCTCATAGTGTTTATTCTCA11.2345679012345678No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGTCCACTTTAAATC11.2345679012345678No Hit
GTCCAGCGCAGCGAGCATGCGGGGCGTGTACTCCCGTTTCTCCAGGGCCT11.2345679012345678No Hit
GTGGTTGTGCTGATCCACACTGGAAGCGTGCGTGCCGTCCCTGGGCTGGA11.2345679012345678No Hit
GGCCAGGCGCGGTGGCTCACACCTGTAATCCCAGCACTTTGGGAGACCGA11.2345679012345678No Hit
CTCTACACCCTGTCGCTTCTGGAACGGCTGCACCGTCACCTTCTCGACGT11.2345679012345678No Hit
GGCCAGCGTGGAGCGACAACTCTGACCCCGCCCTCACCCATCCCTGGGGC11.2345679012345678No Hit
ATTCTATTCGATTCCTTTCGAAGATTATTCCATTTGAGTCCATTCGATGA11.2345679012345678No Hit
GAAAAGAGGAAGTCAAATTGTCCCTGTCTGCAGATGACATGATTGTATAT11.2345679012345678No Hit
TGGAGAGACCGACGGTCAAGGGTTCCGGGGGCAAGGCAACAGTCTCTTCA11.2345679012345678No Hit
CCTCGCGACAAGACGCTGAGGCAGCTGCGCCGGGTGCTGGTCACGCCGGA11.2345679012345678No Hit
TCTCTAAGCCATCCAGTGCCATCCTCGTCGCTGCAGCGACACACGCTCTC11.2345679012345678No Hit
GTCTGAGATTGAGAAGCTGGCCTATCCTGATGACTTGCTGATAAATGGGA11.2345679012345678No Hit
GGTCCATCGTCGGAATCTCCAGCTCGTTGGCCGCCCCGACCAACGCCCCC11.2345679012345678No Hit
GGGCTACGCAGCGGTCTTCGTGCTGAGTGCCGTCCTGTACGCGGCCTGTC11.2345679012345678No Hit
TGACCAGATTTCGCGGGACGCGCAGGACCTTGCCCTCAACGCGGATCTCA11.2345679012345678No Hit
GAATTAATTCTAGGACGATGGGCATGAAACTGTGGTTTGCTCCACAGATT11.2345679012345678No Hit
GTGATCCTGCGCCGCCGGAACCGGGGCAACGCCGCATTCTGCTGCGGCGC11.2345679012345678No Hit
CGCCGACATTTCATCGCTGCCGCGGCAGCGAGCCTTCTCGCCGCGCCGGC11.2345679012345678No Hit
AACGGGTGAACGGCGTCCTCGCGCTGGAGATCATGGGCTTCGCGATCCTC11.2345679012345678No Hit
TGTAATTGGAATGAGTCCACTTTAAATCCTTTAACGAGGATCCATTGGAG11.2345679012345678No Hit
GGCGACGACTCCGCCGGTTTCATCGTCACGACCATCATCGGCATCGCCGG11.2345679012345678No Hit
ACCTCAGCCTCCCAAAGTGCTGAGATTACAGGCGTGAGCCACTGCGCCCA11.2345679012345678No Hit
TGCTTGGCCCGGTACATCGCCAGGTCGGCGTTGCGCAACAGCTCCTCGGC11.2345679012345678No Hit
CAATCTCGCCGCGATCGAAGCCCGTCATCCGGATTGGGCCAGAGGCGAGA11.2345679012345678No Hit
CAGACGCCGAGGTACTGGCTGGGGGATTAGGGGGCTGATGGGAGAGCAAG11.2345679012345678No Hit
GCTTCATGGCGCGGGGGCTCGGGGCGCGCGGGGAACCTGCGGCTCCGAGC11.2345679012345678No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)