FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003647884

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003647884
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81
Sequences flagged as poor quality0
Sequence length146
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCAGGACTGGGCGTGGATCTTCATGGCTGTCCTTCTGGCCTCTGGGCT44.938271604938271No Hit
GTAGTAGGTTTCTTCTCTGCAGGCTTCTTTTCAGGGGCTGGTTTCTTGGT33.7037037037037033No Hit
GTAATAAACGTCACAAGGTGATATGTGGCAAAATTAACCAGCAAACAACC33.7037037037037033No Hit
ATGATAGGAAGAGCCGACATCGAAGGATCAAAAAGCGACGTCGCTATGAA22.4691358024691357No Hit
TGCCGGGCCTGGTCAACGCCACCGAGGAGAACATCCAGACCGACATCCCG22.4691358024691357No Hit
CTCATGCTATTGTTTGTGGTTTAAGAATGCCTTTAAGTGGTTTTCCCCTC22.4691358024691357No Hit
GCTCTCCACCGCGATCCTCGACGTATCGCACGACGGCACCCGCTTCGTCG22.4691358024691357No Hit
GCTAATTTTTGTATTTTTAGTAGAGACGGAGTTTCACCATGTTGGCCAGG22.4691358024691357No Hit
ATCATTGGTTGCCTTTCAATGCGCCGGTGTGGCCGTGGCGTGCGAGCGAG22.4691358024691357No Hit
GGCGAGCACGTGCGCGCGGTGACGAGCCACGGCATGGTGGCCGTCGCGGG22.4691358024691357No Hit
CCCTCAAAGTTCAGGATCACGTCGCCGGCCTGCATCCCGGCATCGAGAGC22.4691358024691357No Hit
GTTTATGAGCTGAAGCAATCTACTTTCTCAGTTTTCAACACTTTTTCTTT22.4691358024691357No Hit
GGCGTCCTGCGCGACCGCGAAGATGCCGGGCATGCCGCCGCCGTCGGCGT22.4691358024691357No Hit
GAGCACGAGCGCACGTGTTAGGACCCGAAAGATGGTGAACTATGCCTGGG22.4691358024691357No Hit
CAGCCCCTCGTCAAGATGGAGTCACTCTGGTTCAAATGCCTCTGACACTA22.4691358024691357No Hit
ATTCCATAGTTGGTCTCATCCCTGGTCAGTTTCCACATGATGATGGTCTT11.2345679012345678No Hit
GTAGTTCTCCTTGAAGAGGTCCTTCACATCCCTTGTAAGTTAGATTCCTA11.2345679012345678No Hit
GAGAAGGAGTTCCCCAAGGTGCGCGTGTGGGGCACGATCGGGTGGATCGT11.2345679012345678No Hit
CTATGAGGTGGTTCACGGGGATGCCCAGGCTGCTCAGCAGGAGCTTCAGC11.2345679012345678No Hit
CTACGGTGCCGAGCGCGCGGGCTGCACCGTCATCCCCATGTCGGGCGGCC11.2345679012345678No Hit
GGCCTTCATGCTGGCCTCCGTCGCCACGCTGGCGCTGCATCCGGACCAGG11.2345679012345678No Hit
GTTCATGCCATTCTCCTGCCTCAGCCTCCCGAGTAGCTGGGGCTACAGGC11.2345679012345678No Hit
CGGGACGGCCGCCCTTGCCTCCACCATCAGCGCCAGGACGCCCGCCGGGC11.2345679012345678No Hit
CCACGAACACGGTATATTTCTCAAGTTCTAACACGTCAGCGTAACTGAGT11.2345679012345678No Hit
ACCCAAGCCGAACGGCTCGTCTGCCTGGAAAGTGGACTTGCGAGGCAGGG11.2345679012345678No Hit
CTTGATGACAGGATCGAACGTGTCAACACCGCCTCCTGCAATGACGGCTT11.2345679012345678No Hit
TGAACTCTTCGACCCACGTCTGGTGCCTCCTGTATCTACTGCCCGACTGA11.2345679012345678No Hit
GAGCACCTCCGTCCGGACGGATTCGGAGCGGATGGCGTCGAGGAGCGCGG11.2345679012345678No Hit
GATGCAAACTTTGCTTGTTGGCCTGACTATCCAGGACTAAGACCCTCTGG11.2345679012345678No Hit
GCCGACGTGGCCGACTTTGGCGACGTTGTCGGTCATCGACTTGTCGTCCC11.2345679012345678No Hit
CTATTTCATTCCATTGGTGTCCATTCCATTCCATTCCTTTCGAGTGCGTT11.2345679012345678No Hit
CCCTCGCAGAGCTCGCGGGTCTCGAACGTGTCGCCGGAGACAAGGTCGGC11.2345679012345678No Hit
CTCTGGTGGTGCATGTAGCCCATCTCCACCAGCTTCTTGAGGTTGTAGCT11.2345679012345678No Hit
AACGCAGAGTACATGGGAATGTACGGCGACCACCGAGATCTACACTCTTT11.2345679012345678Illumina Single End PCR Primer 1 (100% over 29bp)
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT11.2345679012345678No Hit
GAATGTAATCATCAAAGAAAGGAATCGAAGGGAATCATCGAATGGAATCA11.2345679012345678No Hit
GTGCTGACCCACAACGAAGCCACCGGATAGCAGTGGCAGGAAGCGGCAGC11.2345679012345678No Hit
CCTAGAACCAGGCGACCTGCGACTCCTTGACGTTGACAATCGAGTAGTAC11.2345679012345678No Hit
ATGTCCGGATGGCGCTCCGGCTGGCCTGGGCCCATCTCACGCCGCACTTG11.2345679012345678No Hit
CCCTTCCAGCACTGAGAAGCACAGCCACCACACCCACAGCTACCAGCTTT11.2345679012345678No Hit
GCCGCGTACAGGACGGCACTCAGCACGAAGACCGCTGCGTAGCCCCTGTC11.2345679012345678No Hit
TGCACAAACCCATCAGTTTAGGTTGTTCACACACTGTTTGCAAGACCTGC11.2345679012345678No Hit
GTCCTTGTGACGGGCCTGCTGGTTTTCGGTAATTAGCCCATCGCTCGGCG11.2345679012345678No Hit
GTCGCAGGCCGAGACCGATACACGGCTACGGCGGCCGGACCTTTGGGAGC11.2345679012345678No Hit
ATTTCAATCGAGTTGATTCCATTCCATTCCATTCCATTCCATTCGATTCC11.2345679012345678No Hit
CGCCAACACAGTTGGTTGAGATCCGCGTTGAGGGCAAGGTCCTGCGCGTC11.2345679012345678No Hit
GTATTGGTATGATTGATGCCTTTGTTGGTAAGCAGGTGTTTAAGACAGCA11.2345679012345678No Hit
GATGCAAACTTTGCTTGTTGGCCTGACTTTCCAGGACTAAGACCCTCTGG11.2345679012345678No Hit
GATCATAGCTCACTGCAAACTGAAACTCCTGGTCTCTAGCACTCCACCAG11.2345679012345678No Hit
ACCTGAGGCCGTGCGCGCGAGCCGGCACGGCCTTGTCTCCTTGCTGCTCT11.2345679012345678No Hit
GATCTCGGCTCACTGCAATGTCTGCCTCCCGGGTTCAAGCAATTCTCCTG11.2345679012345678No Hit
CGCCCCGAATCGCGGATCACGCGGGCCACGGGGTCGACATGGACCTCCCG11.2345679012345678No Hit
GAACGACCGCTTCGGCCACGACGCCGGGGACGAGCTCCTGCGCACCGTGG11.2345679012345678No Hit
GACCCAGAAGGCGAGCGCGAGGAGGATCGCGAAGCCCATGATCTCCAGCG11.2345679012345678No Hit
CACCCCCTCCTACCTGCACTTACCCAGGGACCACTCCCGGGCTGTCTCTA11.2345679012345678No Hit
GAACATGGAAAGAGATGTCTTTGTTGTTACTCTATATGCCATATAGGCAT11.2345679012345678No Hit
ATTCGACATCGGCTGCCGGATTTCAGCTTCGCCCCGACGAGGTCGCGAGC11.2345679012345678No Hit
CCCCGCGGGACACTCAGCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGG11.2345679012345678No Hit
GAGAGAGTGAGAGAGAACGGAGATAATATCATTGCTCCTGTTTCAGAAGA11.2345679012345678No Hit
CCTCCTCCCAGGTACCAAGAGGGGTCACATTTCCCATTTATCAGCAAGTC11.2345679012345678No Hit
TCTTCGAGGTGAACGAGGCCTTCGCCTCCGTGGTGCTCCGCTTCATGCAG11.2345679012345678No Hit
GCTGCGCCACGGCCGGCGCGGCGAGAAGGCTCGCTGCCGCGGCAGCGATG11.2345679012345678No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)