FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003647899

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003647899
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4805
Sequences flagged as poor quality0
Sequence length146
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTCAGGGTTGTACATAAAAAATCCAGGTTTGTGGAAGTCGCGTTCTTTA350.72840790842872No Hit
GTACATGGGACGCCTCCTCCAAGTCCCAGCGAACCCGCGTGCAACCTGTC270.5619146722164412No Hit
TATCAACGCAGAGTACATGGGACGCCTCCTCCAAGTCCCAGCGAACCCGC260.5411030176899064No Hit
GTCACGGTCAGGGTTGTACATAAAAAATCCAGGTTTGTGGAAGTCGCGTT200.41623309053069724No Hit
GTATCAACGCAGAGTACATGGGACGCCTCCTCCAAGTCCCAGCGAACCCG160.33298647242455776No Hit
CATTATTTCATAGAAAAAGGAATATAGCAAACGGTCACGGTCAGGGTTGT130.2705515088449532No Hit
CTTTACATCTGGGAGCGGGGCTGTCCCAGCATCAGGCGCAGCAGCTGCAC130.2705515088449532No Hit
ACGCAGAGTACATGGGACGCCTCCTCCAAGTCCCAGCGAACCCGCGTGCA120.2497398543184183No Hit
ATTTCATAGAAAAAGGAATATAGCAAACGGTCACGGTCAGGGTTGTACAT120.2497398543184183No Hit
CGGTCAGGGTTGTACATAAAAAATCCAGGTTTGTGGAAGTCGCGTTCTTT120.2497398543184183No Hit
GTACATAAAAAATCCAGGTTTGTGGAAGTCGCGTTCTTTACATCTGGGAG110.22892819979188345No Hit
GAAAAAGGAATATAGCAAACGGTCACGGTCAGGGTTGTACATAAAAAATC110.22892819979188345No Hit
ACGCAGAGTACATGGGACCACGCCTCCTCCAAGTCCCAGCGAACCCGCGT110.22892819979188345No Hit
TATCAACGCAGAGTACATGGGGAAGCCGGCGGTGGACGAGATGTTCCCCG100.20811654526534862No Hit
CTCCAAGTCCCAGCGAACCCGCGTGCAACCTGTCCCGACTCTAGCCGCCT100.20811654526534862No Hit
ATCAACGCAGAGTACATGGGACGCCTCCTCCAAGTCCCAGCGAACCCGCG100.20811654526534862No Hit
TCCCAACTGCTCCTGCGCCGCCGGTGACTCCTGCACCTGCGCCGGCTCCT100.20811654526534862No Hit
GTATCAACGCAGAGTACATGGGATACTGCGAGTATGGCGGCGTCAAAGGT90.18730489073881373No Hit
GTGTTTATTGAGATGGTTTCCCACTCATCTTGACTCAGAGTGCTTTTAGT90.18730489073881373No Hit
ACTCTAGCCGCCTCTTCAGCTCGCCATGGATCCCAACTGCTCCTGCGCCG90.18730489073881373No Hit
GAATATAGCAAACGGTCACGGTCAGGGTTGTACATAAAAAATCCAGGTTT90.18730489073881373No Hit
GTACATGGGGAAGCCGGCGGTGGACGAGATGTTCCCCGAGGGCGCCGGGC90.18730489073881373No Hit
CATTTATTCCGTCCCAGTGGGGAGGGGCAGGGATCCAGGTGGCCGGAGGG80.16649323621227888No Hit
GTACATGGGGCAGCGCCAGGACCTGGCCAACCTGCAGAAGCAGCAGGCCC80.16649323621227888No Hit
GTACATGGGACCACGCCTCCTCCAAGTCCCAGCGAACCCGCGTGCAACCT80.16649323621227888No Hit
GGGCTGTCCCAGCATCAGGCGCAGCAGCTGCACTTGTCCGACGCCCCTTT80.16649323621227888No Hit
AAGGAATATAGCAAACGGTCACGGTCAGGGTTGTACATAAAAAATCCAGG80.16649323621227888No Hit
CCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGTAATTCTAGA80.16649323621227888No Hit
GGTTTGTGGAAGTCGCGTTCTTTACATCTGGGAGCGGGGCTGTCCCAGCA80.16649323621227888No Hit
TCGTAGTTCTGTATCTAAGACCCCAGAAAGTACTTCCTCTATCTTCTGCA70.14568158168574402No Hit
GTACATGGGACTGCGATAGGAATCATGTCTGGTCGCGGCAAAGGCGGAAA70.14568158168574402No Hit
CATCTAGGTCGTTAAGAACAGTCTTATCTTTCTGGTTAACCAGGTCCTAA70.14568158168574402No Hit
CTCCTGCACCTGCGCCGGCTCCTGCAAATGCAAAGAGTGCAAATGCACCT70.14568158168574402No Hit
GTCCTAAATTCAAGGGATTTCCACGTGTTCTTTAAAACCACCTGAAACTG70.14568158168574402No Hit
GTCCCAGCATCAGGCGCAGCAGCTGGACTTGTCCGACGCCCCTTTGCAGA70.14568158168574402No Hit
GGAATATAGCAAACGGTCACGGTCAGGGTTGTACATAAAAAATCCAGGTT70.14568158168574402No Hit
GTGTGTGTGTGTGTGTGTGTAGTTCTCTAGTTTAAAAGCCTTAGGAAGGC70.14568158168574402No Hit
GTACATGGGAAGCCTGCCGCAGGGAGAAGATGGCGGTCGCCGTGAGAACT70.14568158168574402No Hit
CTAGCCGCCTCTTCAGCTCGCCATGGATCCCAACTGCTCCTGCGCCGCCG70.14568158168574402No Hit
ATCCAGGTTTGTGGAAGTCGCGTTCTTTACATCTGGGAGCGGGGCTGTCC70.14568158168574402No Hit
CAGTTATGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTG70.14568158168574402No Hit
CCTTCCAGTCGGGCACGTCCTTCATGATGATGGCCTCCTCCTCCAGGTTC70.14568158168574402No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70.14568158168574402No Hit
GTTGTACATAAAAAATCCAGGTTTGTGGAAGTCGCGTTCTTTACATCTGG70.14568158168574402No Hit
GTACATGGGACAGCAGCAGCACGGCCACTTCCCGGGCTGACATGGCGCCT60.12486992715920915No Hit
GTCTCGCTCTGTCGCCCAGGCCGGACTGCGGACTGCAGTGGCGCAATCTC60.12486992715920915No Hit
ACTTTGGGGTGAGCCCCACAGGAATAAAAAACACTGGGAAGGGGTAACCC60.12486992715920915No Hit
GTGTATGATATGTTTGCGGTTTCGATGATGTGGTCTTTGGAGTAGAAACC60.12486992715920915No Hit
GTATCAACGCAGAGTACATGGGGAGCGCACCGGAAGCGCCCGGCTGGAGG60.12486992715920915No Hit
GTATCAACGCAGAGTACATGGGGCTCTTCTGAGGATCCGGCAAGATGGCA60.12486992715920915No Hit
CTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT60.12486992715920915No Hit
GTATCAACGCAGAGTACATGGGCCTTTCCGGCGGTGACGACCTACGCACA60.12486992715920915No Hit
ACAGCATGCTGGCCATAGGGATTGGAACCCTGATCTACGGGCACTGGAGC60.12486992715920915No Hit
GGTATCAACGCAGAGTACATGGGACGCCTCCTCCAAGTCCCAGCGAACCC60.12486992715920915No Hit
TATCAACGCAGAGTACATGGGATACTGCGAGTATGGCGGCGTCAAAGGTG60.12486992715920915No Hit
GTATCAACGCAGAGTACATGGGACCACGCCTCCTCCAAGTCCCAGCGAAC60.12486992715920915No Hit
GTTCCCCGAGGGCGCCGGGCCCTACGTGGACCTGGACGAGGCGGGAGGCA60.12486992715920915No Hit
CAAATACACCCATTGAAGGAAACCAGGCGGGTGATGCAGCTGCCTCTGCC60.12486992715920915No Hit
GTACATGGGAGGCGGCCGGGAAGATGGCGGACATTCAGACTGAGCGTGCC50.10405827263267431No Hit
CTCCAGAAGAGGAGAAGAGGAAACACAAGAAGAAACGCCTGGTGCAGAGC50.10405827263267431No Hit
TATCAACGCAGAGTACATGGGCCTTTCCGGCGGTGACGACCTACGCACAC50.10405827263267431No Hit
TATCAACGCAGAGTACATGGGGCTCTCCGCCAGACCGCCGCCGCGCCGCC50.10405827263267431No Hit
GTATCAACGCAGAGTACATGGGAGAACTTCCGGCCAGCGCAGCCATTTTG50.10405827263267431No Hit
GATCCCAACTGCTCCTGCGCCGCCGGTGACTCCTGCACCTGCGCCGGCTC50.10405827263267431No Hit
CCTCTGTGGTGCGCAGCCCGTACAGCTCCCCGATCAAGGGGGGCACCCAG50.10405827263267431No Hit
GTATCAACGCAGAGTACATGGGACTGCGATAGGAATCATGTCTGGTCGCG50.10405827263267431No Hit
ACACTGTGGTGTCCAGCAGCATCCGGCTTCATGGGGGGACTTGAACCCTG50.10405827263267431No Hit
ACGTGTACCACATGAAGCCGTGGCTGGCATGGAGAGCCTCCTCTGTGGTG50.10405827263267431No Hit
GGAAGGGCCCCCAGTGGTGCTCAAGACCAGGCAGCAGAAGGCCAGCAGAG50.10405827263267431No Hit
GTACATGGGACGATGGGAAGAGGGGAAAGCCCAGGGGTACAGGAGGCCTC50.10405827263267431No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG50.10405827263267431No Hit
GGTTTCCCACTCATCTTGACTCAGAGTGCTTTTAGTGCTGCTTCCTCCTG50.10405827263267431No Hit
GTATCAACGCAGAGTACATGGGACGATGGGAAGAGGGGAAAGCCCAGGGG50.10405827263267431No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTG50.10405827263267431No Hit
CCTCCAAGTCCCAGCGAACCCGCGTGCAACCTGTCCCGACTCTAGCCGCC50.10405827263267431No Hit
CCTGTAATCTCAGCTACTCAGGAGGCTGAGGCAGGAGAATTGCTTGAACC50.10405827263267431No Hit
CTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCTTCG50.10405827263267431No Hit
GTATCAACGCAGAGTACATGGGCCTGTCGCCACCGAGGTCGCACGCGTGA50.10405827263267431No Hit
CTTTTAGTGCTGCTTCCTCCTGAAGGAACATCCTTCTGTAAGCCTTGCTT50.10405827263267431No Hit
TATCTAGAGTCACCAAAGCCGCCGGCGCCCGCCCCCCGGCCGGGGCCGGA50.10405827263267431No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG50.10405827263267431No Hit
GTCCAGAATCGAACCCTCTCTGCTTCCTAAAGACACGTCTCCTTTAAAGG50.10405827263267431No Hit
ACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGG50.10405827263267431No Hit
GTACATGGGATACTGCGAGTATGGCGGCGTCAAAGGTGAAGCAGGACATG50.10405827263267431No Hit
GTCTCGCTCTGTCGCCCTGGCTGGAGTACAGTGGCACAGACTCAGCTCAC50.10405827263267431No Hit
CACAGATACTCTGATTAACTCTCACAGTGGAGGTTAAGTCCGTCCCCTTC50.10405827263267431No Hit
TATCAACGCAGAGTACATGGGACCACGCCTCCTCCAAGTCCCAGCGAACC50.10405827263267431No Hit
TATCAACGCAGAGTACATGGGCAAGCAGAAGACGGCATACGAGATCTTGC50.10405827263267431Illumina PCR Primer Index 1 (96% over 28bp)
CACCTATGCTTTCATGTTCCATTGCTTTGTTTTGTTCAATCTTTGTTCAC50.10405827263267431No Hit
CTGTGTGTTGAGGTCGCTGTAGACATCCGACCTCTGACCCTGGAGCTCCT50.10405827263267431No Hit
GTATCAACGCAGAGTACATGGGAGCTTTCTCCCGAACGCCAGCGCTGAGG50.10405827263267431No Hit
TATCAACGCAGAGTACATGGGATGACCCAGCGGGCCCCCAGGGGCATCAG50.10405827263267431No Hit
GTACAGACCCTAGACTCAGAAACACAACAGATTTGAGCTAAGACAGCTCT50.10405827263267431No Hit
GGAAGGCAGCGTGGGAGCCGATGACCACCAGGGTGACTCCCAGCACCCAG50.10405827263267431No Hit
TAGAAACCGTCTGAACTATCCTGCCCGCCATCATCCTAGTCCTCATCGCC50.10405827263267431No Hit
CGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCT50.10405827263267431No Hit
AGATTGCCTTGTTCGGCAGCTATCATCAGTGGAGTCTGGCCACACTCATT50.10405827263267431No Hit
GTGACAGACCATTCCCATCGGCCAAGTGGTCGAACCCGACATCCAAGACC50.10405827263267431No Hit
CCTGTAGGCTGGCAGAGGACAGTGGAGCAGCCAACACACAAAACTACCGT50.10405827263267431No Hit
CCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGA50.10405827263267431No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)