FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003647900

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003647900
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4805
Sequences flagged as poor quality0
Sequence length146
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTCAGGGTTGTACATAAAAAATCCAGGTTTGTGGAAGTCGCGTTCTTTA330.6867845993756503No Hit
ACGCAGAGTACATGGGACGCCTCCTCCAAGTCCCAGCGAACCCGCGTGCA230.4786680541103018No Hit
GTACATGGGACGCCTCCTCCAAGTCCCAGCGAACCCGCGTGCAACCTGTC210.437044745057232No Hit
GTATCAACGCAGAGTACATGGGACGCCTCCTCCAAGTCCCAGCGAACCCG190.39542143600416235No Hit
ATTTCATAGAAAAAGGAATATAGCAAACGGTCACGGTCAGGGTTGTACAT180.37460978147762747No Hit
CATTATTTCATAGAAAAAGGAATATAGCAAACGGTCACGGTCAGGGTTGT150.31217481789802287No Hit
GTTTTATTATCATTCACATTATTTCATAGAAAAAGGAATATAGCAAACGG130.2705515088449532No Hit
GTACATGGGGAAGCCGGCGGTGGACGAGATGTTCCCCGAGGGCGCCGGGC130.2705515088449532No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130.2705515088449532No Hit
GTACATGGGACAGCAGCAGCACGGCCACTTCCCGGGCTGACATGGCGCCT120.2497398543184183No Hit
ACTCTAGCCGCCTCTTCAGCTCGCCATGGATCCCAACTGCTCCTGCGCCG120.2497398543184183No Hit
GAATATAGCAAACGGTCACGGTCAGGGTTGTACATAAAAAATCCAGGTTT120.2497398543184183No Hit
GTCCCAGCGAACCCGCGTGCAACCTGTCCCGACTCTAGCCGCCTCTTCAG110.22892819979188345No Hit
CTCCAAGTCCCAGCGAACCCGCGTGCAACCTGTCCCGACTCTAGCCGCCT110.22892819979188345No Hit
GGAATATAGCAAACGGTCACGGTCAGGGTTGTACATAAAAAATCCAGGTT110.22892819979188345No Hit
GGGCTGTCCCTCATCCCGGACCCATCCTCCTCCAGCCAAACCCCCGAGGA100.20811654526534862No Hit
GTACATGGGACCACGCCTCCTCCAAGTCCCAGCGAACCCGCGTGCAACCT100.20811654526534862No Hit
CGGTCAGGGTTGTACATAAAAAATCCAGGTTTGTGGAAGTCGCGTTCTTT100.20811654526534862No Hit
GTATCAACGCAGAGTACATGGGATACTGCGAGTATGGCGGCGTCAAAGGT90.18730489073881373No Hit
CTTTACATCTGGGAGCGGGGCTGTCCCAGCATCAGGCGCAGCAGCTGCAC90.18730489073881373No Hit
GTACATGGGAGCCGCTGCCGTCGCCATGACCCGCGGTAACCAGCGTGAGC90.18730489073881373No Hit
GCATTTATTCCGTCCCAGTGGGGAGGGGCAGGGATCCAGGTGGCCGGAGG90.18730489073881373No Hit
GTACATGGGATACTGCGAGTATGGCGGCGTCAAAGGTGAAGCAGGACATG90.18730489073881373No Hit
TATCAACGCAGAGTACATGGGACCACGCCTCCTCCAAGTCCCAGCGAACC90.18730489073881373No Hit
GTACATGGGACTGCGATAGGAATCATGTCTGGTCGCGGCAAAGGCGGAAA80.16649323621227888No Hit
TCTGGGAGCGGGGCTGTCCCAGCATCAGGCGCAGCAGCTGCACTTGTCCG80.16649323621227888No Hit
GTATCAACGCAGAGTACATGGGCCTGTCGCCACCGAGGTCGCACGCGTGA80.16649323621227888No Hit
GAGTACATGGGACGCCTCCTCCAAGTCCCAGCGAACCCGCGTGCAACCTG80.16649323621227888No Hit
CTCCAGGACTGTGGTCGCCCCGTCCGCTGTGGCGGGAAAGCGGCCCCCAG70.14568158168574402No Hit
ACATCTGGGAGCGGGGCTGTCCCAGCATCAGGCGCAGCAGCTGCACTTGT70.14568158168574402No Hit
GCACTTGTCCGACGCCCCTTTGCAGATGCAGCCCTGGGCACACTTGGCAC70.14568158168574402No Hit
CCTCTGTGGTGCGCAGCCCGTACAGCTCCCCGATCAAGGGGGGCACCCAG70.14568158168574402No Hit
GTCATCATCTGACTCTTCAGACTCCTCCTTCTTCTCATCTTTCTTCTCCT70.14568158168574402No Hit
GTACATAAAAAATCCAGGTTTGTGGAAGTCGCGTTCTTTACATCTGGGAG70.14568158168574402No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGA70.14568158168574402No Hit
TCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCC70.14568158168574402No Hit
TTCCCCGTCACCCGTGGTCACCATGGTAGGCACGGCGACTACCATCGAAA70.14568158168574402No Hit
GTACATGGGCCTGTCGCCACCGAGGTCGCACGCGTGAGACTTCTCCGCCG70.14568158168574402No Hit
TCTCGGGCTTGATCTCCACCTGGTTGAAGGTCTTGCCGTTGTAGACGCCC70.14568158168574402No Hit
GTCCCGACTCTAGCCGCCTCTTCAGCTCGCCATGGATCCCAACTGCTCCT70.14568158168574402No Hit
AAAAGGAATATAGCAAACGGTCACGGTCAGGGTTGTACATAAAAAATCCA70.14568158168574402No Hit
GCATCAGGCGCAGCAGCTGCACTTGTCCGACGCCCCTTTGCAGATGCAGC60.12486992715920915No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG60.12486992715920915No Hit
GGTATCAACGCAGAGTACATGGGACCACGCCTCCTCCAAGTCCCAGCGAA60.12486992715920915No Hit
TACTACATCCATGGCTGTGACAGTTTTGCGCTTGGCGTGCTCCGTATAGG60.12486992715920915No Hit
GTCCCAGCATCAGGCGCAGCAGCTGGACTTGTCCGACGCCCCTTTGCAGA60.12486992715920915No Hit
CCTCCAAGTCCCAGCGAACCCGCGTGCAACCTGTCCCGACTCTAGCCGCC60.12486992715920915No Hit
GGTATCAACGCAGAGTACATGGGACGCCTCCTCCAAGTCCCAGCGAACCC60.12486992715920915No Hit
GAGTAGGATCCAGGGTTAGTTTTTGTAGCCTCGGCTGGCCCGTCGGCCTC60.12486992715920915No Hit
CTCCTGCGCCGCCGGTGACTCCTGCACCTGCGCCGGCTCCTGCAAATGCA60.12486992715920915No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGATGCGTG60.12486992715920915No Hit
ATTTCCACGTGTTCTTTAAAACCACCTGAAACTGTCCTGCGCAGGCTCAC60.12486992715920915No Hit
GTCCCAGCATCAGGCGCAGCAGCTGCACTTGTCCGACGCCCCTTTGCAGA60.12486992715920915No Hit
TCCCAACTGCTCCTGCGCCGCCGGTGACTCCTGCACCTGCGCCGGCTCCT60.12486992715920915No Hit
CCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAGACGTTCG60.12486992715920915No Hit
GTACATGGGATTCCTGCAGAGGATCAAGACAGCACGTGGACCTCGCACAG60.12486992715920915No Hit
GTATCAACGCAGAGTACATGGGACCACGCCTCCTCCAAGTCCCAGCGAAC60.12486992715920915No Hit
CCTTCATCCCTCTCCAGAAGAGGAGAAGAGGAAACACAAGAAGAAACGCC60.12486992715920915No Hit
TATCAACGCAGAGTACATGGGACGCCTCCTCCAAGTCCCAGCGAACCCGC60.12486992715920915No Hit
TCTTTGCATCCTTCAATGACACTTTTGTCCATGTCACTGATCTTTCTGGC50.10405827263267431No Hit
CTCCAGAAGAGGAGAAGAGGAAACACAAGAAGAAACGCCTGGTGCAGAGC50.10405827263267431No Hit
CGCACGGCCGGTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGG50.10405827263267431No Hit
AACTCAGTATGTGAAAAGGCCCCTTATCTTTGTGTGTCCGAGAGATGCTG50.10405827263267431No Hit
GGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCA50.10405827263267431No Hit
GCTGAGAATACAGGCATGCGTCACCATGCCTGGCTAATTTTGTATTTTTA50.10405827263267431No Hit
GTGGTGTCCAGCAGCATCCGGCTTCATGGGGGGACTTGAACCCTGCAGCA50.10405827263267431No Hit
CTGCGAGTATGGCGGCGTCAAAGGTGAAGCAGGACATGCCTCCGCCGGGG50.10405827263267431No Hit
CTGCACTTGTCCGACGCCCCTTTGCAGATGCAGCCCTGGGCACACTTGGC50.10405827263267431No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG50.10405827263267431No Hit
TCCTCCAAGTCCCAGCGAACCCGCGTGCAACCTGTCCCGACTCTAGCCGC50.10405827263267431No Hit
CCTGCAAATGCAAAGAGTGCAAATGCACCTCCTGCAAGAAAAGCTGCTGC50.10405827263267431No Hit
ACATGAAGCCGTGGCTGGCATGGAGAGCCTCCTCTGTGGTGCGCAGCCCG50.10405827263267431No Hit
TGACAGGCATGAGCCACCATGCCTGGCTGAATAAATGTTTTCTTCAAGTG50.10405827263267431No Hit
GTCACGGTCAGGGTTGTACATAAAAAATCCAGGTTTGTGGAAGTCGCGTT50.10405827263267431No Hit
AGCGAGGAGGAGTTGAGAGAACGGAGCGGACGCCATGGCGACCAACATCG50.10405827263267431No Hit
GCCCACAGGGCAGCAGGAGCAGCAGCTTTTCTTGCAGGAGGTGCATTTGC50.10405827263267431No Hit
GTATCAACGCAGAGTACATGGGACGATGGGAAGAGGGGAAAGCCCAGGGG50.10405827263267431No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTG50.10405827263267431No Hit
GTATCAACGCAGAGTACATGGGCCTTTCCGGCGGTGACGACCTACGCACA50.10405827263267431No Hit
TAATGAAGTGGAACCGTGAGCGCAGGCGCCTACAAATCGAGGACTTCGAG50.10405827263267431No Hit
CATTCACATTATTTCATAGAAAAAGGAATATAGCAAACGGTCACGGTCAG50.10405827263267431No Hit
CCCCTATGTTGCTGGTGGCTCTGGCTGTCTTTTTCGGCGCCTGTTACATT50.10405827263267431No Hit
TCAATGCTCAGGAGCCCTGTAATGGCCGGACTGCCCTTCACCTCGCAGTG50.10405827263267431No Hit
TCAGTGAGCTGAATGGAAAAAACATTGAAGACGTCATTGCCCAGGGTATT50.10405827263267431No Hit
GGTATCAACGCAGAGTACATGGGGAAGCCGGCGGTGGACGAGATGTTCCC50.10405827263267431No Hit
GCGCCAAGGACATCAAGAAGATCTTGGACAGCGTGGGTATCGAGGCGGAC50.10405827263267431No Hit
CCCATCAACAAGCCAAATATTCGCCGGTTCCTTTGCTTTCCCTTTTCATC50.10405827263267431No Hit
TTCTTTGACTTTTTAGTGGGAATTTCATCAGTAGGATCTGTTTGTACAGA50.10405827263267431No Hit
ATACAAGTCCATGTTCTTTCAGCCCCTTTGCGCTCATAACGTCAGACGCT50.10405827263267431No Hit
ATCAACGCAGAGTACATGGGCCTTTCCGGCGGTGACGACCTACGCACACG50.10405827263267431No Hit
CTTGATGGACTTGGCAGCCAATGAAAAGGCCGTTCATGCAGACTTTTTTA50.10405827263267431No Hit
TAAAAAATCCAGGTTTGTGGAAGTCGCGTTCTTTACATCTGGGAGCGGGG50.10405827263267431No Hit
GGGTTGTACATAAAAAATCCAGGTTTGTGGAAGTCGCGTTCTTTACATCT50.10405827263267431No Hit
GGCTTTGAACTGGGGAAAAGGGCAGCAATGAGGTGTGGCTGTAGTCCAGG50.10405827263267431No Hit
ACGCAGAGTACATGGGACCACGCCTCCTCCAAGTCCCAGCGAACCCGCGT50.10405827263267431No Hit
AAGTACGCACGGCCGGTACAGTGAAACTGCGAATGGCTCATTAAATCAGT50.10405827263267431No Hit
CCCTAGACTCAGAAACACAACAGATTTGAGCTAAGACAGCTCTGGTGAAA50.10405827263267431No Hit
ACCTGTCCCGACTCTAGCCGCCTCTTCAGCTCGCCATGGATCCCAACTGC50.10405827263267431No Hit
GTCTAGCCCTTGTCGCCCAGGCTGGAGTGCAGTGGCACGATCTCGGCTCA50.10405827263267431No Hit
GGACAGGGTCTCACTTTGTCACCCAGGCTGGAGTACAGTGGGACAACCTT50.10405827263267431No Hit
ATCAACGCAGAGTACATGGGAGGCCTGAGAAGGATTGGCACGGGCACAGA50.10405827263267431No Hit
CTCTTCAGCTCGCCATGGATCCCAACTGCTCCTGCGCCGCCGGTGACTCC50.10405827263267431No Hit
GAGTACATGGGGATGGCCGCCGGGTGTGGTGAGGGCGACGCGCTTGCAGT50.10405827263267431No Hit
AAGTGGAACCGTGAGCGCAGGCGCCTACAAATCGAGGACTTCGAGGCTCG50.10405827263267431No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50.10405827263267431No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)