FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003647943

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003647943
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1329
Sequences flagged as poor quality0
Sequence length146
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT130.9781790820165538No Hit
CCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTTGA110.8276899924755455No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC100.7524454477050414No Hit
TCTTTAAAGGCTGCAGCGTCGTCCTCAAATTTCGCACTGACCATAATGTG80.601956358164033No Hit
CACTTATCCTGGGCTTCATCCAATGGTGGGTGCTAACTCTTTAATAGCCT80.601956358164033No Hit
AGTAAAAGAAGCGGATTTAATTCTGCATTTAATTGATTCTTCAAATGAGG60.4514672686230248No Hit
CGCTGGACCCGATGACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTT60.4514672686230248No Hit
GCTATGAAGAAGACCTCCTGTTTGCCACGCTGGACCCGATGACCAGAAAA60.4514672686230248No Hit
ATTCAGGATCTTCCGACGACATTGATTGCTGCATTCCGCTCAACGCTTGA60.4514672686230248No Hit
GCATAATCCTCATTTGAAGAATCAATTAAATGCAGAATTAAATCCGCTTC60.4514672686230248No Hit
ATTTCGCACTGACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCA60.4514672686230248No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA50.3762227238525207No Hit
CGGCTACAGTGTTCTTCTTTCAGATACAGTAGGATTTATTCAGGATCTTC50.3762227238525207No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA50.3762227238525207No Hit
GATTTATTCAGGATCTTCCGACGACATTGATTGCTGCATTCCGCTCAACG50.3762227238525207No Hit
CCGTAATACTTCAGATCAGTTAAGTAGGGCCATATGCCTCGGGAATAAGC40.3009781790820165No Hit
AATCAACATGGTTCAACCGCCTGACGAGTGCTGACAGCTATGAAGAAGAC40.3009781790820165No Hit
CTTCTATGGCGAATGACAGTGGAAAGCTGTGTGTTGATTTCATGAATGCG40.3009781790820165No Hit
GAAGAAGACCTCCTGTTTGCCACGCTGGACCCGATGACCAGAAAAATGGT40.3009781790820165No Hit
CAGTAGGATTTATTCAGGATCTTCCGACGACATTGATTGCTGCATTCCGC40.3009781790820165No Hit
GTTCAACCGCCTGACGAGTGCTGACAGCTATGAAGAAGACCTCCTGTTTG40.3009781790820165No Hit
GCAATATGCACTGCCGCGTCTGACGGGACAAGGGATCAACCTTTCCCGGC40.3009781790820165No Hit
ATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTTGATCACG40.3009781790820165No Hit
GCGCTGAATCGCTTCTTTAAAGGCTGCAGCGTCGTCCTCAAATTTCGCAC40.3009781790820165No Hit
CAATTAAATGCAGAATTAAATCCGCTTCTTTTACTTCCTCAAGCGTTGAG40.3009781790820165No Hit
GTTTCCAGTTTCGTTTCCCCGGGACCTCTTGCCCCAATTCCTCCGCCTTG40.3009781790820165No Hit
ACATTGATTGCTGCATTCCGCTCAACGCTTGAGGAAGTAAAAGAAGCGGA40.3009781790820165No Hit
CCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTTGATCACGTTTATTG40.3009781790820165No Hit
ATCCAATGGTGGGTGCTAACTCTTTAATAGCCTTCAGTGACTGTGAGATG40.3009781790820165No Hit
CCTCAAGCAGCCGAAGCACTGTTTTTTCATGTCCCGCATAATCCTCATTT40.3009781790820165No Hit
GCACTACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGC40.3009781790820165No Hit
ATCCTCATTTGAAGAATCAATTAAATGCAGAATTAAATCCGCTTCTTTTA40.3009781790820165No Hit
AGTGCTGACAGCTATGAAGAAGACCTCCTGTTTGCCACGCTGGACCCGAT30.2257336343115124No Hit
ACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCGAT30.2257336343115124No Hit
ATCATCTGCTTCAAGCTCCTCAAGCAGCCGAAGCACTGTTTTTTCATGTC30.2257336343115124No Hit
TCTTTAATCCTCTCACTCCACAAATTCATAAGCTTCACCATTTTACTTCA30.2257336343115124No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTGT30.2257336343115124No Hit
ATACAAACGCAGGGAAATCAACATGGTTCAACCGCCTGACGAGTGCTGAC30.2257336343115124No Hit
CTTTCACGGTATCGGCTTCTATGGCGAATGACAGTGGAAAGCTGTGTGTT30.2257336343115124No Hit
ATCCTGGGCTTCATCCAATGGTGGGTGCTAACTCTTTAATAGCCTTCAGT30.2257336343115124No Hit
GTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATACAGTAGGATTTAT30.2257336343115124No Hit
CTCGTGGAAGAGCTTGAAGCTGATCTCCTCATCTTTAATGATGAACTGTC30.2257336343115124No Hit
TCTTTAATGATGAACTGTCGCCAAGTCAGCTGAAGTCATTGGCAACAGCA30.2257336343115124No Hit
ATTCAAGACGTTAACAGTTCTTGGCGCAAATAGCGCTGAATCGCTTCTTT30.2257336343115124No Hit
CCTGACGAGTGCTGACAGCTATGAAGAAGACCTCCTGTTTGCCACGCTGG30.2257336343115124No Hit
CTCTGGGACTATGGACGGGCACGCTCATATCAGGCTATATTTGGTCCGGG30.2257336343115124No Hit
GTCATTCGCCATAGAAGCCGATACCGTGAAAGAAGAAAGAAAAACGGTGT30.2257336343115124No Hit
ATACAGTAGGATTTATTCAGGATCTTCCGACGACATTGATTGCTGCATTC30.2257336343115124No Hit
GTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTAGGGCTGAA30.2257336343115124No Hit
CAGCTATGAAGAAGACCTCCTGTTTGCCACGCTGGACCCGATGACCAGAA30.2257336343115124No Hit
CCATAGAAGCCGATACCGTGAAAGAAGAAAGAAAAACGGTGTGCTTCAAA30.2257336343115124No Hit
CGTGTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC30.2257336343115124No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGGGAAGGCAA30.2257336343115124No Hit
ACAGTGTTCTTCTTTCAGATACAGTAGGATTTATTCAGGATCTTCCGACG30.2257336343115124No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT30.2257336343115124No Hit
GGTTATTATCGTCGCGGTTACCGTAATACTTCAGATCAGTTAAGTAGGGC30.2257336343115124No Hit
CAACAAGGACCTCGTGAAGCGCACCGCCGACAAGCTCAAGATCGTGCCCA30.2257336343115124No Hit
TTCAGATACAGTAGGATTTATTCAGGATCTTCCGACGACATTGATTGCTG30.2257336343115124No Hit
GCAATCGCTCTTGCCGTGCGAGATGCTAGCAAAAAGGTGTACTTCTCAGC30.2257336343115124No Hit
CTTTAAAGGCTGCAGCGTCGTCCTCAAATTTCGCACTGACCATAATGTGA30.2257336343115124No Hit
GTATCAACGCAGAGTACATGGGATACCGTGAAAGAAGAAAGAAAAACGGT20.15048908954100826No Hit
CGGCTATACAAACGCAGGGAAATCAACATGGTTCAACCGCCTGACGAGTG20.15048908954100826No Hit
TCGCACGGATGTTTGGTTAAAGAACAGTCGCAGTTTTCCTCAAATCCCGC20.15048908954100826No Hit
CTGGCACACGGCCGACACGCCGCTCAACAAGGACCTCGTGAAGCGCACCG20.15048908954100826No Hit
CTTTCAGATACAGTAGGATTTATTCAGGATCTTCCGACGACATTGATTGC20.15048908954100826No Hit
CTCTACCAATTGGAGCTTTCTTAGCTGTCTTAGCAGTAGTTTATAAGGAA20.15048908954100826No Hit
CCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTGTTTGA20.15048908954100826No Hit
GTACATGGGATTGTGACTAGTTGACCCTATCACCCCTACTGTGTTCAGAC20.15048908954100826No Hit
GTACATGGGACGGTGTGCTTCAAATTGCGCTTGTCGGCTATACAAACGCA20.15048908954100826No Hit
GCTGACAGCTATGAAGAAGACCTCCTGTTTGCCACGCTGGACCCGATGAC20.15048908954100826No Hit
GTATCAACGCAGAGTACATGGGCCGCCTGACGAGTGCTGACAGCTATGAA20.15048908954100826No Hit
GGCTGCAGCGTCGTCCTCAAATTTCGCACTGACCATAATGTGATCCCTTC20.15048908954100826No Hit
ATCCCGGAAGATACGCTCTAAGCTCGGCAATCGCTCTTGCCGTGCGAGAT20.15048908954100826No Hit
CTATAAGGGCCTAGTACCTTTCGAGCCCCGCGCGTACTAGGCAGATAAGA20.15048908954100826No Hit
TTTAAAGGCTGCAGCGTCGTCCTCAAATTTCGCACTGACCATAATGTGAT20.15048908954100826No Hit
GTACATGGGCCGCCTGACGAGTGCTGACAGCTATGAAGAAGACCTCCTGT20.15048908954100826No Hit
GTTAAGAGCCTCCGCCCGTCTCTGGGACTATGGACGGGCACGCTCATATC20.15048908954100826No Hit
TCTCTGACGAAAACAGCAGACGGAAAAGTACTGACCAGCGTCACACAAAA20.15048908954100826No Hit
TCTTTGATTAGCATGAAACTCCAAGGTCCGGGGGGGTCACTTATCCTGGG20.15048908954100826No Hit
GTCCCGGGGAAACGAAACTGGAAACCGACCGCCGCCATATCAGAAATCGC20.15048908954100826No Hit
TCCGACGACATTGATTGCTGCATTCCGCTCAACGCTTGAGGAAGTAAAAG20.15048908954100826No Hit
GCCCAGCTTGCAGCCTGTCTTGTCCACGGCGAGGAAGGACTTGCCCGAGC20.15048908954100826No Hit
GCGTCTATTTCGGCGCGCAGTACTGGCACACGGCCGACACGCCGCTCAAC20.15048908954100826No Hit
GGAAGATCCTGAATAAATCCTACTGTATCTGAAAGAAGAACACTGTAGCC20.15048908954100826No Hit
AAATTGTGCTTGTCGGCTATACAAACGCAGGGAAATCAACATGGTTCAAC20.15048908954100826No Hit
AGATGTCAATTTAAACGCTATTAAAACCTTGAAAAGTATATTCAATATCC20.15048908954100826No Hit
GTGCTAACTCTTTAATAGCCTTCAGTGACTGTGAGATGCCGTCTACGAGT20.15048908954100826No Hit
GAGTACATGGGCGCTGGACCCGATGACCAGAAAAATGGTCCTGCCAAGCG20.15048908954100826No Hit
TGCGATTTCTGATATGGCGGCGGTCGGTTTCCAGTTTCGTTTCCCCGGGA20.15048908954100826No Hit
AGCTAGCATCTCTGACGAAAACAGCAGACGGAAAAGTACTGACCAGCGTC20.15048908954100826No Hit
GTCTACGAGTGGCACGAATCGCACGGATGTTTGGTTAAAGAACAGTCGCA20.15048908954100826No Hit
GTTAAGTAGGGCCATATGCCTCGGGAATAAGCTGACGGTGACAAGGTTTC20.15048908954100826No Hit
ATGTTGTTGTTAGACACTTCTTTTTGTCTTTAAATATAAGGCGTGGTAGA20.15048908954100826No Hit
ACGCAGAGTACATGGGATACCGTGAAAGAAGAAAGAAAAACGGTGTGCTT20.15048908954100826No Hit
TATTGCAGCTGAGCCAGCTCAATTTGAAGTTTGCCTTCTCTCGTTCTCGC20.15048908954100826No Hit
ACTGTCGCCAAGTCAGCTGAAGTCATTGGCAACAGCAATTGAAGTGAAGA20.15048908954100826No Hit
CCCATAAGGACGGCCAGAAGTGATATAGCAACGTCCTGCAGGAACCAGTT20.15048908954100826No Hit
GTCCGGGGGGGTCACTTATCCTGGGCTTCATCCAATGGTGGGTGCTAACT20.15048908954100826No Hit
GTAGAAATGGGAGTATTTGCACTTGTGGTAACGGTATTTGCATTATTGAT20.15048908954100826No Hit
CCCTGCGTTTGTATAGCCGACAAGCGCAATTTGAAGCACACCGTTTTTCT20.15048908954100826No Hit
CTGCAGCGTCGTCCTCAAATTTCGCACTGACCATAATGTGATCCCTTCCG20.15048908954100826No Hit
ATAGGATTGTAGCCCAGGATGAGTCCCTCAGCGTGACGCAGTAAACTAGT20.15048908954100826No Hit
GCCTAGTACCTTTCGAGCCCCGCGCGCACTAGGCAGATAAGAACCCTCCA20.15048908954100826No Hit
ATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACCGCCCTTAGAC20.15048908954100826No Hit
CTTCAACAACTTCCCTATCTTTAATCCTCTCACTCCACAAATTCATAAGC20.15048908954100826No Hit
GAACATGACGATCCTCATGGCCTGGGCCTCGGGGCTCGAACAGTTCGACG20.15048908954100826No Hit
GTAGCGCAGTCAGCAAACGCGTATCTAGGGAGAAAGTCACAAACCCAAGC20.15048908954100826No Hit
GCTCTAAGCTCGGCAATCGCTCTTGCCGTGCGAGATGCTAGCAAAAAGGT20.15048908954100826No Hit
CCTCTCACTCCACAAATTCATAAGCTTCACCATTTTACTTCACCAATTCC20.15048908954100826No Hit
ATGAAGAAGACCTCCTGTTTGCCACGCTGGACCCGATGACCAGAAAAATG20.15048908954100826No Hit
CATGGGACGGTGTGCTTCAAATTGCGCTTGTCGGCTATACAAACGCAGGG20.15048908954100826No Hit
ATATATAGGAAAAGGGAAGGTAGAAGAGCTGAAGGCACTCGTGGAAGAGC20.15048908954100826No Hit
TACAAACGCAGGGAAATCAACATGGTTCAACCGCCTGACGAGTGCTGACA20.15048908954100826No Hit
GCCTGACGAGTGCTGACAGCTATGAAGAAGACCTCCTGTTTGCCACGCTG20.15048908954100826No Hit
AATCGAGACGCTGCAATAACACAGGGGCATACAGTAACCAGGCAAGAGTT20.15048908954100826No Hit
GCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTCAAGCAGCCGAAG20.15048908954100826No Hit
ATGGACGGGCACGCTCATATCAGGCTATATTTGGTCCGGGTTATTATCGT20.15048908954100826No Hit
AGCTGCTCACAGTATACGGGCGTCGGCATCCTGACCGTCGGCTGATCGTG20.15048908954100826No Hit
AGGCTACTACGTGCGCGATATGACAAGTTAACAAGACGGCGCAGGTTGAT20.15048908954100826No Hit
GAAGATGATTGACCGCACGCAATTGATATTAGATATTTTTGCAAAGCGGG20.15048908954100826No Hit
GGTTGCCACGCTGGACCCGATGACCAGAAAAATGGTCCTGCCAAGCGGCT20.15048908954100826No Hit
GTCGCAATCCTGCGTAATCAACGCCGCAACTTTACGTCGGATTAGCTCTT20.15048908954100826No Hit
GCTTTCCACTGTCATTCGCCATAGAAGCCGATACCGTGAAAGAAGAAAGA20.15048908954100826No Hit
GTACATGGGAGACGACCAGCGCTGACGAGCTGGCGCAATGACGACCTAAT20.15048908954100826No Hit
ATTTGAAGCACACCGTTTTTCTTTCTTCTTTCACGGTATCGGCTTCTATG20.15048908954100826No Hit
GAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATACAGTA20.15048908954100826No Hit
GTAGTAGTGTGGATGTTGTTGTTAGACACTTCTTTTTGTCTTTAAATATA20.15048908954100826No Hit
CATCTGCTTCAAGCTCCTCAAGCAGCCGAAGCACTGTTTTTTCATGTCCC20.15048908954100826No Hit
GTATCAACGCAGAGTACATGGGGGGAATTCCTGGAGATTGTCTCGTACGG20.15048908954100826No Hit
ATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTAGCTCGAG20.15048908954100826No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC20.15048908954100826No Hit
GAATGCAGCAATCAATGTCGTCGGAAGATCCTGAATAAATCCTACTGTAT20.15048908954100826No Hit
CTTCATAGCTGTCAGCACTCGTCAGGCGGTTGAACCATGTTGATTTCCCT20.15048908954100826No Hit
ATCTGAAAGAAGAACACTGTAGCCGCTTGGCAGGACCATTTTTCTGGTCA20.15048908954100826No Hit
CTATGGTTCTATTCCCCAATGACCTTAACTCCGCGATCGACGCTACAAAA20.15048908954100826No Hit
GTCCCGCATAATCCTCATTTGAAGAATCAATTAAATGCAGAATTAAATCC20.15048908954100826No Hit
CGTTAACAGTTCTTGGCGCAAATAGCGCTGAATCGCTTCTTTAAAGGCTG20.15048908954100826No Hit
TCTCTGGGACTATGGACGGGCACGCTCATATCAGGCTATATTTGGTCCGG20.15048908954100826No Hit
GAATTAAATCCGCTTCTTTTACTTCCTCAAGCGTTGAGCGGAATGCAGCA20.15048908954100826No Hit
TAGTAGCCTAGAGCGCCAGGGGCGGAAATTCGCCTGAAAAGTTTTGCCGG20.15048908954100826No Hit
GCAGTGCATATTGCAGCTGAGCCAGCTCAATTTGAAGTTTGCCTTCTCTC20.15048908954100826No Hit
ACTTTACGTCGGATTAGCTCTTCTTTGATTAGCATGAAACTCCAAGGTCC20.15048908954100826No Hit
GCTTGAAGCTGATCTCCTCATCTTTAATGATGAACTGTCGCCAAGTCAGC20.15048908954100826No Hit
CAATCCTGCGTAATCAACGCCGCAACTTTACGTCGGATTAGCTCTTCTTT20.15048908954100826No Hit
CTTTACGTCGGATTAGCTCTTCTTTGATTAGCATGAAACTCCAAGGTCCG20.15048908954100826No Hit
TCAACACACAGCTTTCCACTGTCATTCGCCATAGAAGCCGATACCGTGAA20.15048908954100826No Hit
GCTTCAAATTGCGCTTGTCGGCTATACAAACGCAGGGAAATCAACATGGT20.15048908954100826No Hit
CCTTAGAGATGTAATAGCCCATAACAATAGGAAATATCAGAAATCCAATA20.15048908954100826No Hit
AAGTGGGCAAGCCTGGGGGTCAGCCCAGCTTGCAGCCTGTCTTGTCCACG20.15048908954100826No Hit
AAGTAGGGCCATATGCCTCGGGAATAAGCTGACGGTGACAAGGTTTCCCC20.15048908954100826No Hit
AGGCTGCAGCGTCGTCCTCAAATTTCGCACTGACCATAATGTGATCCCTT20.15048908954100826No Hit
AAGTAAAAGAAGCGGATTTAATTCTGCATTTAATTGATTCTTCAAATGAG20.15048908954100826No Hit
CTCCAGATTACTTCCATTTCCGCCCAAGCTGCTCACAGTATACGGGCGTC20.15048908954100826No Hit
GCTAGCATCTCTGACGAAAACAGCAGACGGAAAAGTACTGACCAGCGTCA20.15048908954100826No Hit
ACAAAAAGAAGTGTCTAACAACAACATCCACACTACTACTCCTTGTCATA20.15048908954100826No Hit
GTATCAACGCAGAGTACATGGGGGGAAACGAAACTGGAAACCGACCGCCG20.15048908954100826No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)