FastQCFastQC Report
Tue 10 May 2022
EGAF00003648229

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003648229
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences155138
Sequences flagged as poor quality0
Sequence length146
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC9390.6052675682295763No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC8620.5556343384599518No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT7090.45701246632030834No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA6720.4331627325349044No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC6400.41253593574752795No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG6340.4086684113498949No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT5820.3751498665704083No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA5760.3712823421727752No Hit
GTATCGGCTTCTATGGCGAATGACAGTGGAAAGCTGTGTGTTGATTTCAT5120.33002874859802245No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC4530.2919980920212972No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT4480.2887751550232696No Hit
GCAATATGCACTGCCGCGTCTGACGGGACAAGGGATCAACCTTTCCCGGC4390.28297386842682No Hit
CCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTTGA4370.28168469362760895No Hit
AGCCGATACCGTGAAAGAAGAAAGAAAAACGGTGTGCTTCAAATTGCGCT4370.28168469362760895No Hit
GCCCTAACTGCGCAGTTAATAATTCTGGCAATTCGTCTCCACACTAGAAG4220.2720158826335263No Hit
CTTCATAGCTGTCAGCACTCGTCAGGCGGTTGAACCATGTTGATTTCCCT4120.26557000863747116No Hit
ATTATTAACTGCGCAGTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTT4010.2584795472418105No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA3980.25654578504299397No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA3730.24043110005285617No Hit
ATCCTACTGTATCTGAAAGAAGAACACTGTAGCCGCTTGGCAGGACCATT3590.23140687645837899No Hit
GTGGAAAGCTGTGTGTTGATTTCATGAATGCGATTTCTGATATGGCGGCG3510.2262501772615349No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT3490.22496100246232387No Hit
ATATATAGGAAAAGGGAAGGTAGAAGAGCTGAAGGCACTCGTGGAAGAGC3420.22044889066508527No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG3410.21980430326547976No Hit
GTTTTTTCATGTCCCGCATAATCCTCATTTGAAGAATCAATTAAATGCAG3370.21722595366705771No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA3350.21593677886784668No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT3320.21400301666903015No Hit
GCCTAGATGTGATACACGTTTCTGGAAACTGCCTCGTCATGCGACTGTTC3250.2094909048717916No Hit
ATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAAACTTCA3250.2094909048717916No Hit
ATATAGATTGGCGCTAGTAGATCACAGAACAACCGCCGCATACGGCCGAT3240.20884631747218607No Hit
CAGTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTA3200.20626796787376397No Hit
GCTACATATATAGGAAAAGGGAAGGTAGAAGAGCTGAAGGCACTCGTGGA3190.20562338047415848No Hit
CCCCTATCCCCTACGAGGCTACCCGGATCGATGACGCGAATTGGGGACAT3080.19853291907849785No Hit
ATACAAACGCAGGGAAATCAACATGGTTCAACCGCCTGACGAGTGCTGAC2920.18821952068480965No Hit
CCCTTAGACGTCTTGGTATACGGACAACTGATGGACCCACGTTGCGAGTC2910.18757493328520414No Hit
GCTGTAAAGAGGGGCGTTGAGTCCGTCCGACTTCACTGCCCCCTTTCAGC2890.18628575848599313No Hit
TCTTTAAAGGCTGCAGCGTCGTCCTCAAATTTCGCACTGACCATAATGTG2850.18370740888757106No Hit
ATCCTGAATAAATCCTACTGTATCTGAAAGAAGAACACTGTAGCCGCTTG2830.18241823408836003No Hit
GTCTTCTTCATAGCTGTCAGCACTCGTCAGGCGGTTGAACCATGTTGATT2830.18241823408836003No Hit
GTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTTCGCCTTGACCGCGGG2800.1804844718895435No Hit
GCTTTCCACTGTCATTCGCCATAGAAGCCGATACCGTGAAAGAAGAAAGA2780.1791952970903325No Hit
ATTTGAAGCACACCGTTTTTCTTTCTTCTTTCACGGTATCGGCTTCTATG2780.1791952970903325No Hit
GAATAAATCCTACTGTATCTGAAAGAAGAACACTGTAGCCGCTTGGCAGG2770.17855070969072698No Hit
ACATATATAGGAAAAGGGAAGGTAGAAGAGCTGAAGGCACTCGTGGAAGA2730.1759723600923049No Hit
GTCTCAGGGGATCTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGC2690.17339401049388287No Hit
CTGTAGCCGCTTGGCAGGACCATTTTTCTGGTCATCGGGTCCAGCGTGGC2680.17274942309427735No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG2600.16759272389743327No Hit
GTACATGGGGGAATTCGAGCTCGCATTTTGAAAATTCTATGGAAGAGCTA2570.1656589616986167No Hit
GCTATGAAGAAGACCTCCTGTTTGCCACGCTGGACCCGATGACCAGAAAA2570.1656589616986167No Hit
ATCCAAGATAGTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTG2560.16501437429901122No Hit
AGGCTACTACGTGCGCGATATGACAAGTTAACAAGACGGCGCAGGTTGAT2500.16114684990137812No Hit
GAGTAATATAGATTGGCGCTAGTAGATCACAGAACAACCGCCGCATACGG2470.1592130877025616No Hit
GTAATATAGATTGGCGCTAGTAGATCACAGAACAACCGCCGCATACGGCC2460.15856850030295608No Hit
TCATAGCTGTCAGCACTCGTCAGGCGGTTGAACCATGTTGATTTCCCTGC2440.15727932550374504No Hit
TTCCAGAGCTGTACGGTCGCACTGAACTCGGATAGGTCTCAGAAAAACGA2380.15341180110611197No Hit
GCATAATCCTCATTTGAAGAATCAATTAAATGCAGAATTAAATCCGCTTC2330.1501888641080844No Hit
CTCGTGGAAGAGCTTGAAGCTGATCTCCTCATCTTTAATGATGAACTGTC2330.1501888641080844No Hit
ATTAAATGCAGAATTAAATCCGCTTCTTTTACTTCCTCAAGCGTTGAGCG2280.14696592711005685No Hit
GTATCTGAAAGAAGAACACTGTAGCCGCTTGGCAGGACCATTTTTCTGGT2280.14696592711005685No Hit
TCTTTAATGATGAACTGTCGCCAAGTCAGCTGAAGTCATTGGCAACAGCA2270.14632133971045133No Hit
GCTTCAAATTGCGCTTGTCGGCTATACAAACGCAGGGAAATCAACATGGT2230.1437429901120293No Hit
GAAAAGTACTGACCAGCGTCACACAAAAACGGAACAGGGCTGACGCCGCT2200.14180922791321274No Hit
GGATATCATCTGCTTCAAGCTCCTCAAGCAGCCGAAGCACTGTTTTTTCA2190.14116464051360725No Hit
ATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAATTT2160.1392308783147907No Hit
GGACAAGGGATCAACCTTTCCCGGCAAGGCGGAGGAATTGGGGCAAGAGG2150.1385862909151852No Hit
GCGTTTGTATAGCCGACAAGCGCAATTTGAAGCACACCGTTTTTCTTTCT2130.13729711611597414No Hit
CTACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCG2120.13665252871636865No Hit
GTAAAGAGGGGCGTTGAGTCCGTCCGACTTCACTGCCCCCTTTCAGCCTT2120.13665252871636865No Hit
GAACTCGGATAGGTCTCAGAAAAACGAAATATAGGCTTACGGTAGGTCCG2110.13600794131676314No Hit
ATGATGAACTGTCGCCAAGTCAGCTGAAGTCATTGGCAACAGCAATTGAA2100.13536335391715762No Hit
ATGCACTGCCGCGTCTGACGGGACAAGGGATCAACCTTTCCCGGCAAGGC2090.1347187665175521No Hit
GTACTGACCAGCGTCACACAAAAACGGAACAGGGCTGACGCCGCTACATA2080.1340741791179466No Hit
GAATTAAATCCGCTTCTTTTACTTCCTCAAGCGTTGAGCGGAATGCAGCA2080.1340741791179466No Hit
GGGCTGACGCCGCTACATATATAGGAAAAGGGAAGGTAGAAGAGCTGAAG2070.1334295917183411No Hit
CAGTAGGATTTATTCAGGATCTTCCGACGACATTGATTGCTGCATTCCGC2050.13214041691913006No Hit
AGGTAGAAGAGCTGAAGGCACTCGTGGAAGAGCTTGAAGCTGATCTCCTC2020.1302066547203135No Hit
CTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGCTTCCTTCCGCAG2020.1302066547203135No Hit
GGTGTACACCGCCCTTAGACGTCTTGGTATACGGACAACTGATGGACCCA2010.12956206732070802No Hit
GAAGATGATTGACCGCACGCAATTGATATTAGATATTTTTGCAAAGCGGG2000.1289174799211025No Hit
CAATTGAAGTGAAGATGATTGACCGCACGCAATTGATATTAGATATTTTT2000.1289174799211025No Hit
GTTGATTTCCCTGCGTTTGTATAGCCGACAAGCGCAATTTGAAGCACACC1970.12698371772228598No Hit
GATGATTGACCGCACGCAATTGATATTAGATATTTTTGCAAAGCGGGCGA1970.12698371772228598No Hit
CTGCCCTAACTGCGCAGTTAATAATTCTGGCAATTCGTCTCCACACTAGA1960.12633913032268046No Hit
ATTCAAATGAGCATCCTAGTCACCGCGTTTAAAATGAACCTGCCGGCTGA1950.12569454292307494No Hit
CACTAGAAGTCGACGAACAACGAAGAGCGACGATGCCCGTTTCAGGTGGT1930.12440536812386392No Hit
GGCTTACGGTAGGTCCGAATGGCACAAAGCTTGTTCCGTTAGCTGGCATA1930.12440536812386392No Hit
ATCCTCATTTGAAGAATCAATTAAATGCAGAATTAAATCCGCTTCTTTTA1910.12311619332465289No Hit
CCTCAAGCAGCCGAAGCACTGTTTTTTCATGTCCCGCATAATCCTCATTT1910.12311619332465289No Hit
GAATGCAGCAATCAATGTCGTCGGAAGATCCTGAATAAATCCTACTGTAT1900.12247160592504737No Hit
ATGTTGATTTCCCTGCGTTTGTATAGCCGACAAGCGCAATTTGAAGCACA1880.12118243112583635No Hit
CCGCTACATATATAGGAAAAGGGAAGGTAGAAGAGCTGAAGGCACTCGTG1870.12053784372623084No Hit
GTAGAAGAGCTGAAGGCACTCGTGGAAGAGCTTGAAGCTGATCTCCTCAT1840.1186040815274143No Hit
GTGCTAGCCCGTCGAGACTGAAAAGCTATAACCCGCAGACCCGAGCGAAA1820.11731490672820329No Hit
TGACTGGACACTGCATCGGAAGACACTTCGTGCCGACACGAAGCAGCCTC1820.11731490672820329No Hit
GCTTGAAGCTGATCTCCTCATCTTTAATGATGAACTGTCGCCAAGTCAGC1820.11731490672820329No Hit
GCATATTGCAGCTGAGCCAGCTCAATTTGAAGTTTGCCTTCTCTCGTTCT1790.11538114452938673No Hit
GTCGCCAAGTCAGCTGAAGTCATTGGCAACAGCAATTGAAGTGAAGATGA1790.11538114452938673No Hit
CCTCATCTTTAATGATGAACTGTCGCCAAGTCAGCTGAAGTCATTGGCAA1780.11473655712978123No Hit
CTCCAGATTACTTCCATTTCCGCCCAAGCTGCTCACAGTATACGGGCGTC1770.11409196973017571No Hit
TCATGCGACTGTTCCCCGGGGTCAGGGCCGCTGGTATTTGCTGTAAAGAG1760.11344738233057021No Hit
GCTGCAATATGCACTGCCGCGTCTGACGGGACAAGGGATCAACCTTTCCC1750.11280279493096469No Hit
CTTTACAGCAAATACCAGCGGCCCTGACCCCGGGGAACAGTCGCATGACG1740.11215820753135919No Hit
GTTTTTGATCACGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCT1730.11151362013175367No Hit
GGCTAGACTAGCGTACGAGCACTATGGTCAGTAATTCCTGGAGGAATAGG1730.11151362013175367No Hit
ATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTCAAGCA1720.11086903273214817No Hit
ATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAAAC1700.10957985793293712No Hit
CATTTGAAGAATCAATTAAATGCAGAATTAAATCCGCTTCTTTTACTTCC1670.1076460957341206No Hit
GGAAAAGGGAAGGTAGAAGAGCTGAAGGCACTCGTGGAAGAGCTTGAAGC1670.1076460957341206No Hit
GCTTCAAGCTCCTCAAGCAGCCGAAGCACTGTTTTTTCATGTCCCGCATA1650.10635692093490956No Hit
ATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTTGATCACG1640.10571233353530406No Hit
ACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCGAT1640.10571233353530406No Hit
GCGAATGACAGTGGAAAGCTGTGTGTTGATTTCATGAATGCGATTTCTGA1640.10571233353530406No Hit
GAACGAGAGAAGGCAAACTTCAAATTGAGCTGGCTCAGCTGCAATATGCA1620.10442315873609304No Hit
GAAGAACACTGTAGCCGCTTGGCAGGACCATTTTTCTGGTCATCGGGTCC1610.1037785713364875No Hit
GTATAGCCGACAAGCGCAATTTGAAGCACACCGTTTTTCTTTCTTCTTTC1600.10313398393688199No Hit
ACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTTG1600.10313398393688199No Hit
TATCAATTGCGTGCGGTCAATCATCTTCACTTCAATTGCTGTTGCCAATG1600.10313398393688199No Hit
GCTGTGTGTTGATTTCATGAATGCGATTTCTGATATGGCGGCGGTCGGTT1600.10313398393688199No Hit
GCGTACGGCGGGACCTCTGAGAATTGGGATACAGGACCCAAAAGGCTGAA1590.10248939653727648No Hit
TCTTTTACTTCCTCAAGCGTTGAGCGGAATGCAGCAATCAATGTCGTCGG1580.10184480913767097No Hit
GATTTCATGAATGCGATTTCTGATATGGCGGCGGTCGGTTTCCAGTTTCG1570.10120022173806546No Hit
GTGTTGATTTCATGAATGCGATTTCTGATATGGCGGCGGTCGGTTTCCAG1570.10120022173806546No Hit
ACACACAGCTTTCCACTGTCATTCGCCATAGAAGCCGATACCGTGAAAGA1570.10120022173806546No Hit
CGTTAACAGTTCTTGGCGCAAATAGCGCTGAATCGCTTCTTTAAAGGCTG1570.10120022173806546No Hit
ATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGGCT1560.10055563433845995No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)