FastQCFastQC Report
Wed 8 May 2024
EGAF00003648445

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003648445
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1207172
Total Bases176.2 Mbp
Sequences flagged as poor quality0
Sequence length146
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTCTATAGAGCTCACACCCATACTCCCAGCTGGGACCCAGCCTTCCCTG38690.3205011381973737No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT15300.1267425023111868No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA14500.12011544336681101No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC13630.11290851676480236No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT12740.1055359136891843No Hit
TCCTGAGAGTGCCCATCGGCTGAAGTCAAGCATCGGGCTGATCAATGAAA12480.10338211953226219No Hit
TCCTAGTGCCATCCCTGCCCAATCTAGTGAAAAACCAGCTGTGTCCCGTG12230.10131116361214475No Hit
GAGTATAAGTTGGTCACCTTTGGTGTAAAACTGAGCTTCCGAGCTGGCTT12200.10106264890173067No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)