FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003648658

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003648658
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62
Sequences flagged as poor quality0
Sequence length146
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGTAGGTCAGCGCTGTGTTCCCGTGGGCATCTCGGGCCATGACGTCCAC34.838709677419355No Hit
GTGGTATATAGTTTGTTAAATGTCAACAAAGTATACTCATGACACTAAGT34.838709677419355No Hit
GATGAATATTATCGGATGAAGAAGGAGGAAGAAGAAGAAGAAGAAGAAAA34.838709677419355No Hit
GTAGTACACGTCATCTGGTTGAAGTGCAAAATATTTGTACAAGTATGCTC23.225806451612903No Hit
CTGTAAGGCACATGGAGGTGATGATGTTTCTTAGAGAGAAGATCACTGAA23.225806451612903No Hit
GTCCTTGTGCGGTCTTCCCTCCATGCACACACATCTCTAAGTTTCATTAT23.225806451612903No Hit
GGATTATTGCTAGGATGAGGATGGATAGTAATAGGGCAAGGACGCCTCCT23.225806451612903No Hit
TGCATACCCTGATACCAAATTAGTGTAAGTACTGTGTAAGAAAAAAACAA23.225806451612903No Hit
GGGTTTTCCTGAAGTAGCCAGATAGATCGTGTTTACATTATTAGCATTTC23.225806451612903No Hit
CTCTTTGAGAGCGTACACTCCCGCGTGCGCGACACCAGGCGGCCCGTAGG23.225806451612903No Hit
TCCTTCATTATACGGGATGGTTTTGCAAGAATAAGACTTGACCAAGACCG11.6129032258064515No Hit
CTCCATATTAGTGCTACCAAAGTGTCATTAATTTCTATTTGTTGGAAACT11.6129032258064515No Hit
CCTGTACACAGGCTTTTCCATCCTGGTGACTCTGCTCCTCGCTGACCAGG11.6129032258064515No Hit
TGCTTAACCTAGATTGTCTTCAAAAACTATTAAAATGTAAAAGACTTAAC11.6129032258064515No Hit
CACACACACACACACACACACACACACACAAGGGCATATAATGTAGGAGT11.6129032258064515No Hit
CGCTGAGGAAGAGGGACTTTCTTTAGATGTCGTATCCGGAGGAGAGCTAT11.6129032258064515No Hit
GTCCTGGGTTGGTTCTTTTAGCAGTCATTTTGCTGTTGGTGAGAGCAGCA11.6129032258064515No Hit
TCAGTATTCTCCCTGATGACCCCCGACAAATGACTCTCTTATTCTAAGGA11.6129032258064515No Hit
CTCTCAGGCTGTGTCCAGCAACAGTGCCCAGGGCTCTGACTGTCTCTTAT11.6129032258064515No Hit
GTTGTAGGTGATTAAAATAATTTGAAGGCGATCTTTTAAAAAGAGATTAA11.6129032258064515No Hit
TCATGACACAGTCGATAAATAAAGAAACCCAGGTGATCAATGTTTTCAAT11.6129032258064515No Hit
GAATAGTATTAGTCGGTCAGTGGACCCCCTCCTGAATATCTATACTTAGC11.6129032258064515No Hit
CTTCTAAACAGAGCAGCAGCAGCAGAGATGATAACATGTTTCAGGAATTA11.6129032258064515No Hit
CTCTTTGGGTCAGAGTTAAAGCCAGGGTTGGACAAAAAGAATCTGCCTAT11.6129032258064515No Hit
GTCGTCATCCTTAAACACTGTGCTGAAGACTACTGTGACTCTGTCCTTTT11.6129032258064515No Hit
TTCCAGGTTCGCACTTTCAGGTGTAGCTGGGGGCAGTAAGTCGAATTGTT11.6129032258064515No Hit
GCAAAAGATTCAAAAACTGCTTTAAGTCATGCTTATGCTGCTGGTGCCAG11.6129032258064515No Hit
GCCCCTTTGTCACGAACTCACCTTCCAGGCATGGTATGTGCCCGAGGGGT11.6129032258064515No Hit
GTCTCCCACCACCACACACTTGATGGCCTGCATCAGGGCTGTCTCTTATA11.6129032258064515No Hit
ACACTGAATTCACCCCCACTGAAAAAGATGAGTATGCCTGCCGTGTGAAC11.6129032258064515No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACCCT11.6129032258064515No Hit
CCTCAGCTCTCCCTCTGCAGAGTTCCCTACCCTAAGAGAATGTTACCACC11.6129032258064515No Hit
TTCAGATACAGTAGGATTTATTCAGGATCTTCCGACGACATTGATTGCTG11.6129032258064515No Hit
ATCCTGGCCATGGTGGGTGAAGACGCTGATCTGCCCTGTCACCTTTCCCG11.6129032258064515No Hit
GTATAGCCGTACTCCTGATTTGTGTACATTGTTTTTGTATCCTGAAACTT11.6129032258064515No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAG11.6129032258064515No Hit
ACCCTGCGGAGATCACACTGACCTGGCAGCGGGATGGCGAGGACCAAACT11.6129032258064515No Hit
CCACAATGTACAGCCAGCCAATTTGATACATTTCACAGTAATTTGGCTAT11.6129032258064515No Hit
TGCAAGGACTCTGAATTTTTGCAGGGCTATTTTTGGATACTGTAAAAAAA11.6129032258064515No Hit
ACTCCAAAGATTCAGGTTTACTCACGTCATCCAGCAGAGAATGGAAAGTC11.6129032258064515No Hit
ATAGTAGACTTTATAGAGAAAGACCAGTTTTACCTAGATACTAAAGGAAG11.6129032258064515No Hit
GAGAGAGTTCAAACCATTTACTAAGCAGATTCTTAGCCTTCCCACTCCCG11.6129032258064515No Hit
GACATGAGGATCCACAGGGGCACAAACACCACCAGCCACGGCCAGTGAAT11.6129032258064515No Hit
TCATTGGGGAGTGATGCTGGTGAAAGGGAAGAGAGTGGCTCAGCCTGCAG11.6129032258064515No Hit
GTTCTAATATGCTGCAAGCAGGTAAACAAAGTCAGTTTCACTGTCTAAAT11.6129032258064515No Hit
GTACTGTACTAGACAGCCCATTCTTCTCACTAATGCTCACAATCCAAGAG11.6129032258064515No Hit
TCTCGGACCCGTCGCAACGGTTGTTAATATGCTGTCTCTTATACACATCT11.6129032258064515No Hit
GAGTACATGGGAGACAAGCTCACCGAAATGGCCGCGTCCAGTCAAGGAAA11.6129032258064515No Hit
CGCCCCAACCGAAATTTTTAATGCAGGTTTGGTAGTTTAGGACCTGTGGG11.6129032258064515No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)