FastQCFastQC Report
Tue 10 May 2022
EGAF00003648941

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003648941
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41798
Sequences flagged as poor quality0
Sequence length146
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTTATACTGGTCCAAGCAAGCATTAGGTCATAGCTTGTTCCTCTTCCT1370.3277668787980286No Hit
GTGCAGCACCACGCGTGTGTCAACGTAGCACCCTCAACGATCGAAACCCA1160.2775252404421264No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1120.2679554045648117No Hit
CTACAACACGGCGCTGGTCGCCTCGCTCACGCGCACGACGGCGAAGCCAA870.20814393033159484No Hit
GTGCTGCACTGACCGCGGTTCGGCGGTGCCCACCGCGCGCTGGGGTGGAC850.20335901239293747No Hit
GTATAAGCATGCCAGGGCAAATATTTAGGCTAAATTGTGGGAGCTAAGAA810.19378917651562277No Hit
GTCTGCATCCGTGCCGGCCAGCACCCCTACAACACGGCGCTGGTCGCCTC710.169864586822336No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC680.16268720991434998No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT650.15550983300636395No Hit
GGCTAAAAATGTATATGCCCAAAGATCTTCTTAAAGAACTGGTACCTGTA640.15311737403703526No Hit
CTATAGAGAAGTGCTGAGCAGAAGCGCTCCATTTAGTCAACGGCTAATCC630.15072491506770658No Hit
GACTTAGAGTACCCCGTGCGCAAGGGCGTCGCGCTCGATCCCGTCATTCG610.14593999712904923No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG590.14115507919039189No Hit
GTACATGGGAGTGCGGGTGCAGACAATCTTCTGTTGGGTGGCATCATTCC570.13637016125173454No Hit
CCCCTACAACACGGCGCTGGTCGCCTCGCTCACGCGCACGACGGCGAAGC560.13397770228240585No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT510.12201540743576249No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT500.1196229484664338No Hit
GTATATGGGTACAGCCGCAGGTAGCTTGATGGATTCTCTGGTGGGAAAAC500.1196229484664338No Hit
CTCTAAGTCCGCCTGCGCGTACAGCTGCTGGGCCGGCTCGGACACCAGGA500.1196229484664338No Hit
GTACATGGGCAAGGTCTGCATCCGTGCCGGCCAGCACCCCTACAACACGG490.11723048949710511No Hit
ATCCAATAACGACACCCGCAGACTGCCGCAAGCCATGCGCTCCAGGCGCT480.11483803052777646No Hit
CTCGTACTACGTGGGCCAGATGAAGTCGTCCAAGGAAGGCAGCGACGCGC480.11483803052777646No Hit
GTAATAAAGAGATCAATGTATTTTATGTTCTTAGCTCCCACAATTTAGCC470.11244557155844777No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA460.1100531125891191No Hit
GGTTATAAATGACCCTGTCTCCTTTTTTCGGTGTACTCTTATGGCAGAAC460.1100531125891191No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT460.1100531125891191No Hit
GTACATGGGAGGGCCGCTGGTATTTGCTGTAAAGAGGGGCGTTGAGTCCG450.10766065361979041No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC450.10766065361979041No Hit
AGCATGAACAGTGCACCTGGGACAGGGAGCAGCCCCAAATTGTCACCTGC450.10766065361979041No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA440.10526819465046175No Hit
GGGTACTCTAAGTCCGCCTGCGCGTACAGCTGCTGGGCCGGCTCGGACAC440.10526819465046175No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA430.10287573568113306No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG420.1004832767118044No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT420.1004832767118044No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)