FastQCFastQC Report
Tue 10 May 2022
EGAF00003648942

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003648942
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41798
Sequences flagged as poor quality0
Sequence length146
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCAGCACCACGCGTGTGTCAACGTAGCACCCTCAACGATCGAAACCCA1130.2703478635341404No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1020.24403081487152498No Hit
TGCTTATACTGGTCCAAGCAAGCATTAGGTCATAGCTTGTTCCTCTTCCT1000.2392458969328676No Hit
GTGCTGCACTGACCGCGGTTCGGCGGTGCCCACCGCGCGCTGGGGTGGAC860.20575147136226613No Hit
CTCTAAGTCCGCCTGCGCGTACAGCTGCTGGGCCGGCTCGGACACCAGGA800.1913967175462941No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC740.17704196373032202No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT730.17464950476099333No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT640.15311737403703526No Hit
CTACAACACGGCGCTGGTCGCCTCGCTCACGCGCACGACGGCGAAGCCAA620.14833245609837792No Hit
CTATAGAGAAGTGCTGAGCAGAAGCGCTCCATTTAGTCAACGGCTAATCC610.14593999712904923No Hit
GTCTGCATCCGTGCCGGCCAGCACCCCTACAACACGGCGCTGGTCGCCTC600.14354753815972054No Hit
AGCATGAACAGTGCACCTGGGACAGGGAGCAGCCCCAAATTGTCACCTGC600.14354753815972054No Hit
GTACATGGGAGTGCGGGTGCAGACAATCTTCTGTTGGGTGGCATCATTCC600.14354753815972054No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA540.1291927843437485No Hit
GTCCGATGCGCGGGCCCGCGTTATCCAATAACGACACCCGCAGACTGCCG510.12201540743576249No Hit
GTAATAAAGAGATCAATGTATTTTATGTTCTTAGCTCCCACAATTTAGCC500.1196229484664338No Hit
CATATAACCACATTCAAATTGACCTCCCTCAGGAAGCTAAGAAATACTAT500.1196229484664338No Hit
GTATAAGCATGCCAGGGCAAATATTTAGGCTAAATTGTGGGAGCTAAGAA490.11723048949710511No Hit
GTACATGGGCTGTGTCACGTGCCATGGTCACTCATATTTGGCTCAGAATA490.11723048949710511No Hit
ATCCAATAACGACACCCGCAGACTGCCGCAAGCCATGCGCTCCAGGCGCT490.11723048949710511No Hit
GGGTACTCTAAGTCCGCCTGCGCGTACAGCTGCTGGGCCGGCTCGGACAC490.11723048949710511No Hit
GACTTAGAGTACCCCGTGCGCAAGGGCGTCGCGCTCGATCCCGTCATTCG490.11723048949710511No Hit
GTCCTAGAATCTGCAGGTGTGAACTTGAAACAGGAATCCATTTGGGGCAA480.11483803052777646No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA480.11483803052777646No Hit
GTATATGGGTACAGCCGCAGGTAGCTTGATGGATTCTCTGGTGGGAAAAC480.11483803052777646No Hit
CCCCTACAACACGGCGCTGGTCGCCTCGCTCACGCGCACGACGGCGAAGC470.11244557155844777No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA460.1100531125891191No Hit
GAATAAATCTCTTCAAATATTTCACAAAGTTAGACTCTTTTCGTGGACAG460.1100531125891191No Hit
CTACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCG450.10766065361979041No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGATGCGTG440.10526819465046175No Hit
GTACATGGGCAAGGTCTGCATCCGTGCCGGCCAGCACCCCTACAACACGG440.10526819465046175No Hit
TTTATAACCTGATGCATCCACCTCACTACTGTGTCCAGTTTCCATTGGCT430.10287573568113306No Hit
TTGCTGGCCTGTGCGATGGTGCTTCTGGGCATCTGGATCGGTAGTCGGCG420.1004832767118044No Hit
GTGTAAAGTATATGGGTACAGCCGCAGGTAGCTTGATGGATTCTCTGGTG420.1004832767118044No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC420.1004832767118044No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)