FastQCFastQC Report
Tue 10 May 2022
EGAF00003649304

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003649304
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1050284
Sequences flagged as poor quality0
Sequence length146
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCCATCCTCTCCCCAAGCTCCCATTTCTCCAGGGCTTGTCCCTGCGGT24400.2323181158619954No Hit
GTGTATGACTACCAGCAGCTGCTGATCGCCTACAAGCCAGCCCCTGGCAC20150.191852870271279No Hit
TCGCCACACAGGGTGCTCACGGCCATGCCCAGGAAGGCCGGGTCCCCTCC19190.18271248538490542No Hit
GTTCTGGAGATGAGCATTGGGGCGCCGGAAGCCCAGCAACGCCTGGCCCT18140.1727151894154343No Hit
TTTCTAGTGAGAGCCTCAAGACTGGGGATGCTCTCTGGAGCTATCTTCAT15640.1489121037738364No Hit
GCTCATGGGTCTCCACATGAGCCAGAAACACACGGAGATGGTTCTGGAGA15390.14653179520967663No Hit
GTCCTAGATGTAGTAGAGCGGCACCTCGCCACACAGGGTGCTCACGGCCA14900.14186639042392343No Hit
CTCTGGAGCTATCTTCATGATGTAGCAGCAGGTGCCAGGGGCTGGCTTGT13420.12777496372409747No Hit
ATGTAGTAGAGCGGCACCTCGCCACACAGGGTGCTCACGGCCATGCCCAG13400.1275845390389647No Hit
GTCATACACCACGAGGCCAGTGGAGCCGATGGAGAAGGTGGCAGTGGTAA13360.12720368966869913No Hit
GCATAGCACCTGCAGCAAGATGGATGTGGGCAGCAAAGAGGTCCTGATGG13040.12415689470657461No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA12540.11939627757825502No Hit
CCTCCGGAGGGTGCTGAGCCTGCATCTCGCCCCTCTGCCTGGCCCAGCTT12270.11682554432896246No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT12120.11539735919046659No Hit
GCTTAGACGTAGGCACTGCGGGCTTGGCCTGGAAGTTGTGGACTTTTCTA11790.11225535188577565No Hit
GTACATGGGAGGAGAGGAGAGCATAGCACCTGCAGCAAGATGGATGTGGG11650.11092237908984619No Hit
CCTCTGCCCACTCCCATCCTCTCCCCAAGCTCCCATTTCTCCAGGGCTTG11400.10854207052568639No Hit
CCTCAAGACTGGGGATGCTCTCTGGAGCTATCTTCATGATGTAGCAGCAG11390.10844685818311998No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC11170.10635218664665938No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT11160.10625697430409299No Hit
ATGTAGCAGCAGGTGCCAGGGGCTGGCTTGTAGGCGATCAGCAGCTGCTG11110.10578091259126103No Hit
CTGCTGCCCAGTGCACCTGAAACGCCTTCTTATCGTGGTGGTGGTGGTGG11080.10549527556356186No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)