FastQCFastQC Report
Tue 10 May 2022
EGAF00003649526

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003649526
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7973
Sequences flagged as poor quality0
Sequence length146
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC350.4389815627743635No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC240.3010159287595635No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC240.3010159287595635No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT230.2884735983945817No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT220.2759312680295999No Hit
GTATCGGCTTCTATGGCGAATGACAGTGGAAAGCTGTGTGTTGATTTCAT220.2759312680295999No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA210.2633889376646181No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG180.22576194656967266No Hit
ATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAAACTTCA180.22576194656967266No Hit
CTTCATAGCTGTCAGCACTCGTCAGGCGGTTGAACCATGTTGATTTCCCT170.21321961620469082No Hit
GCAATATGCACTGCCGCGTCTGACGGGACAAGGGATCAACCTTTCCCGGC170.21321961620469082No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170.21321961620469082No Hit
CCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTTGA160.20067728583970904No Hit
CTACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCG160.20067728583970904No Hit
ATACAAACGCAGGGAAATCAACATGGTTCAACCGCCTGACGAGTGCTGAC150.1881349554747272No Hit
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA150.1881349554747272No Hit
ATGCACTGCCGCGTCTGACGGGACAAGGGATCAACCTTTCCCGGCAAGGC150.1881349554747272No Hit
TATCAACGCAGAGTACATGGGAATGATACGGCGACCACCGAGACTGTCTC150.1881349554747272Illumina Single End PCR Primer 1 (95% over 22bp)
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA150.1881349554747272No Hit
AGCCGATACCGTGAAAGAAGAAAGAAAAACGGTGTGCTTCAAATTGCGCT150.1881349554747272No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG140.17559262510974538No Hit
ATTCAAATGAGCATCCTAGTCACCGCGTTTAAAATGAACCTGCCGGCTGA140.17559262510974538No Hit
GCCCTAACTGCGCAGTTAATAATTCTGGCAATTCGTCTCCACACTAGAAG140.17559262510974538No Hit
ATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAATTT140.17559262510974538No Hit
CTGCCCTAACTGCGCAGTTAATAATTCTGGCAATTCGTCTCCACACTAGA130.16305029474476357No Hit
GTGGAAAGCTGTGTGTTGATTTCATGAATGCGATTTCTGATATGGCGGCG130.16305029474476357No Hit
GCGTTTGTATAGCCGACAAGCGCAATTTGAAGCACACCGTTTTTCTTTCT130.16305029474476357No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA130.16305029474476357No Hit
CCTCAAGCAGCCGAAGCACTGTTTTTTCATGTCCCGCATAATCCTCATTT130.16305029474476357No Hit
GTATCAACGCAGAGTACATGGGAATGATACGGCGACCACCGAGACTGTCT130.16305029474476357Illumina Single End PCR Primer 1 (95% over 22bp)
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA130.16305029474476357No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG130.16305029474476357No Hit
GATTTCATGAATGCGATTTCTGATATGGCGGCGGTCGGTTTCCAGTTTCG120.15050796437978176No Hit
GTATCAACGCAGAGTACATGGGAATGATACGGCGACCACCGAGATCCTGT120.15050796437978176Illumina Single End PCR Primer 1 (95% over 24bp)
ATCCTACTGTATCTGAAAGAAGAACACTGTAGCCGCTTGGCAGGACCATT120.15050796437978176No Hit
GCCGAAGCACTGTTTTTTCATGTCCCGCATAATCCTCATTTGAAGAATCA120.15050796437978176No Hit
CAGTAGGATTTATTCAGGATCTTCCGACGACATTGATTGCTGCATTCCGC120.15050796437978176No Hit
GTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTTCGCCTTGACCGCGGG120.15050796437978176No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC120.15050796437978176No Hit
GTCTCAGGGGATCTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGC120.15050796437978176No Hit
ACTGGAAACCGACCGCCGCCATATCAGAAATCGCATTCATGAAATCAACA110.13796563401479994No Hit
GAAGAAGACCTCCTGTTTGCCACGCTGGACCCGATGACCAGAAAAATGGT110.13796563401479994No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA110.13796563401479994No Hit
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA110.13796563401479994No Hit
CTGTAGCCGCTTGGCAGGACCATTTTTCTGGTCATCGGGTCCAGCGTGGC110.13796563401479994No Hit
GCTTTCCACTGTCATTCGCCATAGAAGCCGATACCGTGAAAGAAGAAAGA110.13796563401479994No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT110.13796563401479994No Hit
ATCCTAGTCACCGCGTTTAAAATGAACCTGCCGGCTGATCGTTTTTAGGA110.13796563401479994No Hit
GAAAAGTACTGACCAGCGTCACACAAAAACGGAACAGGGCTGACGCCGCT110.13796563401479994No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110.13796563401479994No Hit
TCTTTAAAGGCTGCAGCGTCGTCCTCAAATTTCGCACTGACCATAATGTG110.13796563401479994No Hit
AGGCTACTACGTGCGCGATATGACAAGTTAACAAGACGGCGCAGGTTGAT110.13796563401479994No Hit
GTCCCTCAGCGTGACGCAGTAAACTAGTAGCATCCCGATCTTTTCCCTAT110.13796563401479994No Hit
CATATGCAGATCCCCTGAGACAGAGTCGCTATCGTTATGTCTCCTTCCCG100.12542330364981813No Hit
CGTCTGAACTCCAGTCACGAAGCAAGATCTCGTATGCCGTCTTCTGCTTG100.12542330364981813Illumina PCR Primer Index 1 (96% over 28bp)
ACATATATAGGAAAAGGGAAGGTAGAAGAGCTGAAGGCACTCGTGGAAGA100.12542330364981813No Hit
ATCATATCCAAGATAGTGTAGGACTCGTCGCATTGGATGACGATGCCTAG100.12542330364981813No Hit
GAACTCGGATAGGTCTCAGAAAAACGAAATATAGGCTTACGGTAGGTCCG100.12542330364981813No Hit
ATTTGAAGCACACCGTTTTTCTTTCTTCTTTCACGGTATCGGCTTCTATG100.12542330364981813No Hit
ATGTTGATTTCCCTGCGTTTGTATAGCCGACAAGCGCAATTTGAAGCACA100.12542330364981813No Hit
GATGATTGACCGCACGCAATTGATATTAGATATTTTTGCAAAGCGGGCGA100.12542330364981813No Hit
GTATCTGAAAGAAGAACACTGTAGCCGCTTGGCAGGACCATTTTTCTGGT100.12542330364981813No Hit
CGTTAACAGTTCTTGGCGCAAATAGCGCTGAATCGCTTCTTTAAAGGCTG100.12542330364981813No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT100.12542330364981813No Hit
ATCTTTTCCCTATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTG100.12542330364981813No Hit
CCCTGAGACAGAGTCGCTATCGTTATGTCTCCTTCCCGCGGTCAAGGCGA100.12542330364981813No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA100.12542330364981813No Hit
AATATGCACTGCCGCGTCTGACGGGACAAGGGATCAACCTTTCCCGGCAA90.11288097328483633No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA90.11288097328483633No Hit
AATCAACATGGTTCAACCGCCTGACGAGTGCTGACAGCTATGAAGAAGAC90.11288097328483633No Hit
TCATAGCTGTCAGCACTCGTCAGGCGGTTGAACCATGTTGATTTCCCTGC90.11288097328483633No Hit
CCGCTACATATATAGGAAAAGGGAAGGTAGAAGAGCTGAAGGCACTCGTG90.11288097328483633No Hit
CAGCTGAAGTCATTGGCAACAGCAATTGAAGTGAAGATGATTGACCGCAC90.11288097328483633No Hit
TAACAAGACGGCGCAGGTTGATGCTTCCAATAAACATGATCTTTCTGCTC90.11288097328483633No Hit
TTCCAGAGCTGTACGGTCGCACTGAACTCGGATAGGTCTCAGAAAAACGA90.11288097328483633No Hit
GCTACATATATAGGAAAAGGGAAGGTAGAAGAGCTGAAGGCACTCGTGGA90.11288097328483633No Hit
GTTGAACCATGTTGATTTCCCTGCGTTTGTATAGCCGACAAGCGCAATTT90.11288097328483633No Hit
ACGCTTGAGGAAGTAAAAGAAGCGGATTTAATTCTGCATTTAATTGATTC90.11288097328483633No Hit
ACACACAGCTTTCCACTGTCATTCGCCATAGAAGCCGATACCGTGAAAGA90.11288097328483633No Hit
ATCGTTATGTCTCCTTCCCGCGGTCAAGGCGAAACCGCAGCAAACTTCCT90.11288097328483633No Hit
GCATCGGGATATCATCTGCTTCAAGCTCCTCAAGCAGCCGAAGCACTGTT90.11288097328483633No Hit
ACCCGGATCGATGACGCGAATTGGGGACATTCAAATGAGCATCCTAGTCA90.11288097328483633No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90.11288097328483633No Hit
GTATGTCCCAAGGGGACTTAAGCGCGGTGGCCTCCCCTATCCCCTACGAG80.10033864291985452No Hit
ACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCGAT80.10033864291985452No Hit
CCTCATCTTTAATGATGAACTGTCGCCAAGTCAGCTGAAGTCATTGGCAA80.10033864291985452No Hit
GTCTTCTTCATAGCTGTCAGCACTCGTCAGGCGGTTGAACCATGTTGATT80.10033864291985452No Hit
TATTGCAGCTGAGCCAGCTCAATTTGAAGTTTGCCTTCTCTCGTTCTCGC80.10033864291985452No Hit
CGTTTGTATAGCCGACAAGCGCAATTTGAAGCACACCGTTTTTCTTTCTT80.10033864291985452No Hit
GTCCAGTCATGACCTCGATCCATCGCGTATAGGGACGCCCCCTGCTCGCG80.10033864291985452No Hit
GTTTTTTCATGTCCCGCATAATCCTCATTTGAAGAATCAATTAAATGCAG80.10033864291985452No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA80.10033864291985452Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCTGCAATATGCACTGCCGCGTCTGACGGGACAAGGGATCAACCTTTCCC80.10033864291985452No Hit
ATCTAATATCAATTGCGTGCGGTCAATCATCTTCACTTCAATTGCTGTTG80.10033864291985452No Hit
GGAAAGCTGTGTGTTGATTTCATGAATGCGATTTCTGATATGGCGGCGGT80.10033864291985452No Hit
GTCATTCGCCATAGAAGCCGATACCGTGAAAGAAGAAAGAAAAACGGTGT80.10033864291985452No Hit
CGGTTGAACCATGTTGATTTCCCTGCGTTTGTATAGCCGACAAGCGCAAT80.10033864291985452No Hit
TATCAACGCAGAGTACATGGGAATGATACGGCGACCACCGAGATCCTGTC80.10033864291985452Illumina Single End PCR Primer 1 (95% over 24bp)
CGGCTATACAAACGCAGGGAAATCAACATGGTTCAACCGCCTGACGAGTG80.10033864291985452No Hit
ATATAGATTGGCGCTAGTAGATCACAGAACAACCGCCGCATACGGCCGAT80.10033864291985452No Hit
GAATGCAGCAATCAATGTCGTCGGAAGATCCTGAATAAATCCTACTGTAT80.10033864291985452No Hit
CATCTAGGCATGGAATCTTATGCCAGCTAACGGAACAAGCTTTGTGCCAT80.10033864291985452No Hit
CTCGTCAGGCGGTTGAACCATGTTGATTTCCCTGCGTTTGTATAGCCGAC80.10033864291985452No Hit
GGATATCATCTGCTTCAAGCTCCTCAAGCAGCCGAAGCACTGTTTTTTCA80.10033864291985452No Hit
GTATCAACGCAGAGTACATGGGAATGATACGGCGACCACCGAGATCTGTC80.10033864291985452Illumina Single End PCR Primer 1 (96% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)