FastQCFastQC Report
Wed 11 May 2022
EGAF00003649942

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003649942
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3404419
Sequences flagged as poor quality0
Sequence length146
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT59380.17442036365089023No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA58440.1716592464088586No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC53310.15659059592840952No Hit
TCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGGCGTTATT50730.1490122103066632No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT50190.14742603657187908No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA48240.14169818697404754No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCG46630.13696903935737642No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA38890.11423388249213742No Hit
CCCTCACCCTGGTCACACTGGCTCTCTGCTGCAGCTCCGCTTCTGCAGAG38850.11411638814141267No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA38380.11273582952039686No Hit
CTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCAACC36800.10809480266676928No Hit
GTTGGGGATCTTCAGCTTCTAAATGCTAATTACACAGTGAGCTTTGGGCT35880.10539243260010005No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA35150.10324816069937337No Hit
AGACAGGCCAGAGCATCCCCCTCCTCCACCATGAAACTCGCTGTCACCCT35060.1029837984102427No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)