FastQCFastQC Report
Tue 10 May 2022
EGAF00003649991

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003649991
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences91290
Sequences flagged as poor quality0
Sequence length146
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCGGCTTCTATGGCGAATGACAGTGGAAAGCTGTGTGTTGATTTCAT5220.5718041406506736No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA4990.5466097053346478No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC4760.521415270018622No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC4240.4644539379997809No Hit
GCAATATGCACTGCCGCGTCTGACGGGACAAGGGATCAACCTTTCCCGGC3600.3943476832073612No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT3480.38120276043378243No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT3010.3297184795705992No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC2760.30233322379231026No Hit
AGCCGATACCGTGAAAGAAGAAAGAAAAACGGTGTGCTTCAAATTGCGCT2500.2738525577828897No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT2360.2585168145470479No Hit
ATCCTACTGTATCTGAAAGAAGAACACTGTAGCCGCTTGGCAGGACCATT2340.25632599408478474No Hit
GTGGAAAGCTGTGTGTTGATTTCATGAATGCGATTTCTGATATGGCGGCG2330.25523058385365316No Hit
ATTTGAAGCACACCGTTTTTCTTTCTTCTTTCACGGTATCGGCTTCTATG2190.23989484061781138No Hit
ATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAAACTTCA2190.23989484061781138No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA2110.2311315587687589No Hit
AGGCTACTACGTGCGCGATATGACAAGTTAACAAGACGGCGCAGGTTGAT2020.22127286668857488No Hit
CAGTAGGATTTATTCAGGATCTTCCGACGACATTGATTGCTGCATTCCGC1970.21579581553291707No Hit
GCCCTAACTGCGCAGTTAATAATTCTGGCAATTCGTCTCCACACTAGAAG1950.21360499507065397No Hit
GAATAAATCCTACTGTATCTGAAAGAAGAACACTGTAGCCGCTTGGCAGG1850.2026508927593384No Hit
CTACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCG1840.20155548252820682No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA1820.1993646620659437No Hit
GCGTTTGTATAGCCGACAAGCGCAATTTGAAGCACACCGTTTTTCTTTCT1820.1993646620659437No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG1790.19607843137254902No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT1720.18841055975462812No Hit
CTTCATAGCTGTCAGCACTCGTCAGGCGGTTGAACCATGTTGATTTCCCT1690.18512432906123344No Hit
GCTTTCCACTGTCATTCGCCATAGAAGCCGATACCGTGAAAGAAGAAAGA1670.1829335085989703No Hit
GGACAAGGGATCAACCTTTCCCGGCAAGGCGGAGGAATTGGGGCAAGAGG1660.18183809836783876No Hit
CCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTTGA1650.18074268813670719No Hit
ACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCGAT1620.1774564574433125No Hit
GAAGATGATTGACCGCACGCAATTGATATTAGATATTTTTGCAAAGCGGG1620.1774564574433125No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA1570.17197940628765473No Hit
CTGTAGCCGCTTGGCAGGACCATTTTTCTGGTCATCGGGTCCAGCGTGGC1560.17088399605652316No Hit
ATATATAGGAAAAGGGAAGGTAGAAGAGCTGAAGGCACTCGTGGAAGAGC1550.1697885858253916No Hit
GCTACATATATAGGAAAAGGGAAGGTAGAAGAGCTGAAGGCACTCGTGGA1550.1697885858253916No Hit
GTACATGGGGGAATTCGAGCTCGCATTTTGAAAATTCTATGGAAGAGCTA1530.16759776536312848No Hit
TCTTTAATGATGAACTGTCGCCAAGTCAGCTGAAGTCATTGGCAACAGCA1520.16650235513199693No Hit
GTCTTCTTCATAGCTGTCAGCACTCGTCAGGCGGTTGAACCATGTTGATT1510.16540694490086538No Hit
ATCCTGAATAAATCCTACTGTATCTGAAAGAAGAACACTGTAGCCGCTTG1500.1643115346697338No Hit
GCATATTGCAGCTGAGCCAGCTCAATTTGAAGTTTGCCTTCTCTCGTTCT1490.16321612443860226No Hit
ATACAAACGCAGGGAAATCAACATGGTTCAACCGCCTGACGAGTGCTGAC1480.1621207142074707No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA1470.16102530397633916No Hit
GTTGATTTCCCTGCGTTTGTATAGCCGACAAGCGCAATTTGAAGCACACC1450.158834483514076No Hit
GGATATCATCTGCTTCAAGCTCCTCAAGCAGCCGAAGCACTGTTTTTTCA1450.158834483514076No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG1430.1566436630518129No Hit
ATGTTGATTTCCCTGCGTTTGTATAGCCGACAAGCGCAATTTGAAGCACA1410.15445284258954978No Hit
GCGAATGACAGTGGAAAGCTGTGTGTTGATTTCATGAATGCGATTTCTGA1410.15445284258954978No Hit
GTTTTTTCATGTCCCGCATAATCCTCATTTGAAGAATCAATTAAATGCAG1400.15335743235841823No Hit
ACATATATAGGAAAAGGGAAGGTAGAAGAGCTGAAGGCACTCGTGGAAGA1390.15226202212728668No Hit
CCCCTATCCCCTACGAGGCTACCCGGATCGATGACGCGAATTGGGGACAT1390.15226202212728668No Hit
TATCAATTGCGTGCGGTCAATCATCTTCACTTCAATTGCTGTTGCCAATG1380.15116661189615513No Hit
ATGATGAACTGTCGCCAAGTCAGCTGAAGTCATTGGCAACAGCAATTGAA1380.15116661189615513No Hit
ATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAAAC1370.15007120166502355No Hit
GCATAATCCTCATTTGAAGAATCAATTAAATGCAGAATTAAATCCGCTTC1370.15007120166502355No Hit
CCATAGAAGCCGATACCGTGAAAGAAGAAAGAAAAACGGTGTGCTTCAAA1370.15007120166502355No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG1360.14897579143389197No Hit
GTCGCCAAGTCAGCTGAAGTCATTGGCAACAGCAATTGAAGTGAAGATGA1350.14788038120276042No Hit
GCTATGAAGAAGACCTCCTGTTTGCCACGCTGGACCCGATGACCAGAAAA1350.14788038120276042No Hit
ATATAGATTGGCGCTAGTAGATCACAGAACAACCGCCGCATACGGCCGAT1340.14678497097162888No Hit
ATGCACTGCCGCGTCTGACGGGACAAGGGATCAACCTTTCCCGGCAAGGC1320.14459415050936575No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT1310.1434987402782342No Hit
ATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAATTT1300.14240333004710265No Hit
TCTTTAAAGGCTGCAGCGTCGTCCTCAAATTTCGCACTGACCATAATGTG1280.14021250958483952No Hit
GCTGCAATATGCACTGCCGCGTCTGACGGGACAAGGGATCAACCTTTCCC1280.14021250958483952No Hit
GGTCCAGCGTGGCAAACAGGAGGTCTTCTTCATAGCTGTCAGCACTCGTC1270.13911709935370797No Hit
GTTGAACCATGTTGATTTCCCTGCGTTTGTATAGCCGACAAGCGCAATTT1260.1380216891225764No Hit
CTCGTGGAAGAGCTTGAAGCTGATCTCCTCATCTTTAATGATGAACTGTC1260.1380216891225764No Hit
GTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTTCGCCTTGACCGCGGG1250.13692627889144485No Hit
CTTCAAATTGAGCTGGCTCAGCTGCAATATGCACTGCCGCGTCTGACGGG1250.13692627889144485No Hit
GTATCTGAAAGAAGAACACTGTAGCCGCTTGGCAGGACCATTTTTCTGGT1240.13583086866031327No Hit
TGACTGGACACTGCATCGGAAGACACTTCGTGCCGACACGAAGCAGCCTC1220.13364004819805017No Hit
GCTGTGTGTTGATTTCATGAATGCGATTTCTGATATGGCGGCGGTCGGTT1220.13364004819805017No Hit
TTCAGATACAGTAGGATTTATTCAGGATCTTCCGACGACATTGATTGCTG1210.13254463796691862No Hit
GCTTCAAATTGCGCTTGTCGGCTATACAAACGCAGGGAAATCAACATGGT1210.13254463796691862No Hit
GTGTTGATTTCATGAATGCGATTTCTGATATGGCGGCGGTCGGTTTCCAG1210.13254463796691862No Hit
CGGCTACAGTGTTCTTCTTTCAGATACAGTAGGATTTATTCAGGATCTTC1200.13144922773578707No Hit
GAATTAAATCCGCTTCTTTTACTTCCTCAAGCGTTGAGCGGAATGCAGCA1170.12816299704239237No Hit
AGGTAGAAGAGCTGAAGGCACTCGTGGAAGAGCTTGAAGCTGATCTCCTC1160.12706758681126082No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC1160.12706758681126082No Hit
CTCAAGCGTTGAGCGGAATGCAGCAATCAATGTCGTCGGAAGATCCTGAA1140.12487676634899769No Hit
ATCCCGGAAGATACGCTCTAAGCTCGGCAATCGCTCTTGCCGTGCGAGAT1130.12378135611786614No Hit
GTATGTCCCAAGGGGACTTAAGCGCGGTGGCCTCCCCTATCCCCTACGAG1130.12378135611786614No Hit
CCTCAAGCAGCCGAAGCACTGTTTTTTCATGTCCCGCATAATCCTCATTT1120.1226859458867346No Hit
GATTTCATGAATGCGATTTCTGATATGGCGGCGGTCGGTTTCCAGTTTCG1110.12159053565560303No Hit
GAACTCGGATAGGTCTCAGAAAAACGAAATATAGGCTTACGGTAGGTCCG1110.12159053565560303No Hit
GATGATTGACCGCACGCAATTGATATTAGATATTTTTGCAAAGCGGGCGA1110.12159053565560303No Hit
GCCTAGATGTGATACACGTTTCTGGAAACTGCCTCGTCATGCGACTGTTC1110.12159053565560303No Hit
CCCTTGTCCCGTCAGACGCGGCAGTGCATATTGCAGCTGAGCCAGCTCAA1100.12049512542447145No Hit
ATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTTGATCACG1090.1193997151933399No Hit
CTTCTATGGCGAATGACAGTGGAAAGCTGTGTGTTGATTTCATGAATGCG1090.1193997151933399No Hit
GTGTGTTGATTTCATGAATGCGATTTCTGATATGGCGGCGGTCGGTTTCC1080.11830430496220835No Hit
GTCCAGTCATGACCTCGATCCATCGCGTATAGGGACGCCCCCTGCTCGCG1080.11830430496220835No Hit
GAATGCAGCAATCAATGTCGTCGGAAGATCCTGAATAAATCCTACTGTAT1080.11830430496220835No Hit
GCTTGAAGCTGATCTCCTCATCTTTAATGATGAACTGTCGCCAAGTCAGC1080.11830430496220835No Hit
GTAGAAGAGCTGAAGGCACTCGTGGAAGAGCTTGAAGCTGATCTCCTCAT1070.11720889473107679No Hit
GCTTCAAGCTCCTCAAGCAGCCGAAGCACTGTTTTTTCATGTCCCGCATA1060.11611348449994523No Hit
ATCCTCATTTGAAGAATCAATTAAATGCAGAATTAAATCCGCTTCTTTTA1060.11611348449994523No Hit
CGTCAGGCGGTTGAACCATGTTGATTTCCCTGCGTTTGTATAGCCGACAA1050.11501807426881366No Hit
TCTTTTACTTCCTCAAGCGTTGAGCGGAATGCAGCAATCAATGTCGTCGG1040.11392266403768211No Hit
CAATTGAAGTGAAGATGATTGACCGCACGCAATTGATATTAGATATTTTT1040.11392266403768211No Hit
GAAAAGTACTGACCAGCGTCACACAAAAACGGAACAGGGCTGACGCCGCT1030.11282725380655056No Hit
ACCTTTCCCGGCAAGGCGGAGGAATTGGGGCAAGAGGTCCCGGGGAAACG1030.11282725380655056No Hit
CCCCGGGACCTCTTGCCCCAATTCCTCCGCCTTGCCGGGAAAGGTTGATC1020.11173184357541899No Hit
TCATAGCTGTCAGCACTCGTCAGGCGGTTGAACCATGTTGATTTCCCTGC1020.11173184357541899No Hit
GCTGCATTCCGCTCAACGCTTGAGGAAGTAAAAGAAGCGGATTTAATTCT1020.11173184357541899No Hit
CATTTGAAGAATCAATTAAATGCAGAATTAAATCCGCTTCTTTTACTTCC1010.11063643334428744No Hit
CGTTTGTATAGCCGACAAGCGCAATTTGAAGCACACCGTTTTTCTTTCTT1010.11063643334428744No Hit
GTATAGCCGACAAGCGCAATTTGAAGCACACCGTTTTTCTTTCTTCTTTC1000.10954102311315587No Hit
GAATTGGGGCAAGAGGTCCCGGGGAAACGAAACTGGAAACCGACCGCCGC1000.10954102311315587No Hit
CGGTTGAACCATGTTGATTTCCCTGCGTTTGTATAGCCGACAAGCGCAAT990.10844561288202433No Hit
GAAGAAGACCTCCTGTTTGCCACGCTGGACCCGATGACCAGAAAAATGGT970.1062547924197612No Hit
GAAGAACACTGTAGCCGCTTGGCAGGACCATTTTTCTGGTCATCGGGTCC970.1062547924197612No Hit
ACAGTGGAAAGCTGTGTGTTGATTTCATGAATGCGATTTCTGATATGGCG970.1062547924197612No Hit
GCTGTAAAGAGGGGCGTTGAGTCCGTCCGACTTCACTGCCCCCTTTCAGC970.1062547924197612No Hit
GTCATTCGCCATAGAAGCCGATACCGTGAAAGAAGAAAGAAAAACGGTGT970.1062547924197612No Hit
ACATTGATTGCTGCATTCCGCTCAACGCTTGAGGAAGTAAAAGAAGCGGA960.10515938218862964No Hit
GGATCAACCTTTCCCGGCAAGGCGGAGGAATTGGGGCAAGAGGTCCCGGG960.10515938218862964No Hit
GTAATATAGATTGGCGCTAGTAGATCACAGAACAACCGCCGCATACGGCC960.10515938218862964No Hit
GTGCATATTGCAGCTGAGCCAGCTCAATTTGAAGTTTGCCTTCTCTCGTT960.10515938218862964No Hit
ATTAAATGCAGAATTAAATCCGCTTCTTTTACTTCCTCAAGCGTTGAGCG950.10406397195749809No Hit
ATCCAAGATAGTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTG950.10406397195749809No Hit
ATTCAGGATCTTCCGACGACATTGATTGCTGCATTCCGCTCAACGCTTGA940.10296856172636654No Hit
CACTGTAGCCGCTTGGCAGGACCATTTTTCTGGTCATCGGGTCCAGCGTG940.10296856172636654No Hit
ATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTCAAGCA940.10296856172636654No Hit
GGAAAAGGGAAGGTAGAAGAGCTGAAGGCACTCGTGGAAGAGCTTGAAGC940.10296856172636654No Hit
GAGTAATATAGATTGGCGCTAGTAGATCACAGAACAACCGCCGCATACGG930.10187315149523496No Hit
CCCCAATTCCTCCGCCTTGCCGGGAAAGGTTGATCCCTTGTCCCGTCAGA920.10077774126410341No Hit
ATCGCAGACGGTATCAACAGGAACATCCTCGTGTTAGATATTGAGGCTGC920.10077774126410341No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)