FastQCFastQC Report
Tue 10 May 2022
EGAF00003650180

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003650180
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences429966
Sequences flagged as poor quality0
Sequence length146
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT16160.375843671360061No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA15190.35328374801728507No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCG14430.33560793178995546No Hit
TCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGGCGTTATT14270.33188670732104397No Hit
CTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCAACC13870.3225836461487652No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT12460.2897903555164827No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCCGGAAGCTGCCCG10220.23769321295172177No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA9280.21583101919686673No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC8640.20094612132122075No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAG8380.19489913155923957No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA8140.18931729485587231No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATCTGTCAAT8030.18675895303349568No Hit
ACCATAAACGATGCCGACCGGCGATGCGGCGGCGTTATTCCCATGACCCG8010.18629379997488174No Hit
GTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAAGAGGGACGGC7990.1858286469162678No Hit
CTCGGGGGTCGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGG7960.18513091732834688No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA7930.184433187740426No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC7870.1830377285645842No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA7690.17885135103705874No Hit
GCGTTATTCCCATGACCCGCCGGGCAGCTTCCGGGAAACCAAAGTCTTTG7610.176990738802603No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC7430.17280436127507756No Hit
CTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTG7250.16861798374755213No Hit
GTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGT7090.16489675927864064No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA6930.16117553480972913No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGGGCACCAC6420.14931413181507375No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA6330.14722094305131103No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA6110.14210425940655774No Hit
GTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGACCAG6100.14187168287725077No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAAC6050.1407088002307159No Hit
CCCAAAGACTTTGGTTTCCCGGAAGCTGCCCGGCGGGTCATGGGAATAAC6020.14001107064279503No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT5860.1362898461738835No Hit
GAATAACGCCGCCGCATCGCCGGTCGGCATCGTTTATGGTCGGAACTACG5850.13605726964457654No Hit
AGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAAGA5650.13140573905843717No Hit
ATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATCTGTCAATCCT5570.12954512682398142No Hit
ATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGGACGCC5560.12931255029467445No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTGGTGCATG5500.12791709111883265No Hit
GACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAAGAACGAA5410.12582390235506993No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGAT5360.12466101970853509No Hit
TTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGAC5330.12396329012061419No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG5150.11977691259308874No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGATGCGTG4870.11326476977249363No Hit
TCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGC4810.11186931059665184No Hit
TCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCC4810.11186931059665184No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTACTTTGAAAAAAT4790.11140415753803788No Hit
CATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGG4780.11117158100873092No Hit
GTTCGAAGACGATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGA4730.1100086983621961No Hit
CCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCG4710.10954354530358214No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC4690.10907839224496821No Hit
CCCATGACCCGCCGGGCAGCTTCCGGGAAACCAAAGTCTTTGGGTTCCGG4520.10512459124674975No Hit
GACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAA4510.10489201471744276No Hit
GTTTATGGTCGGAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACT4480.10419428512952186No Hit
GCTAGGAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAA4460.10372913207090793No Hit
TTCTTGGACCGGCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATG4460.10372913207090793No Hit
GCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGT4310.10024048413130342No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)