FastQCFastQC Report
Wed 31 Aug 2022
EGAF00003650188

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003650188
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15
Sequences flagged as poor quality0
Sequence length146
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCGGAAGGCGTTGAGCTGCTGATGACCGGCGGGCATTTGCGCCGTCAGT320.0No Hit
ACTGCACGCCGACGGCCGTCACGGCGTCCTCGTCGGAGCTGCTGCCGGCC213.333333333333334No Hit
CGCCGTTTTGCTGGTGCAGGATGAAGGTTTTACTGACGTTTTGTACGTTA213.333333333333334No Hit
CCCACAAACAAGCGGGGGTCGGGCAAATTGGTGCCGGGATCACCACTGCG213.333333333333334No Hit
GTGCCCTCGTGACGACCGTCACCTGCTCAGCGACCCGATGGCCGGGCTTT16.666666666666667No Hit
AGCGCATCCTCAAGGACTTCTTCACCTTCCGTCCCAGGAAAGGCCAGCGT16.666666666666667No Hit
GCATCAGCTTCTACGTCAAGGGCGTGGAGGGCCAGGTGCCCGGCGGCGAC16.666666666666667No Hit
GTCCAGCGCCGAGGTGGGCTCGTCGCAGACGAGGAACTCCGGGCTGGTGG16.666666666666667No Hit
GTGCAGGAGGACTGATGAGCGCCTCATGGTCGTGGGCTTGTGTGCGGGCC16.666666666666667No Hit
TGCTGCCGGTGAAGCCTTCGATGGCGTGCTTGTAGAGCGTGTCCTTGCCC16.666666666666667No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)