FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003650300

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003650300
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences122
Sequences flagged as poor quality0
Sequence length146
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTCAGGACTGTTGCCTCAAGTACAGCCAAAGGAAGATTCCCGCCAAGGT54.098360655737705No Hit
GGCCTGACCCCGGCGGCCCTGTCCGCCCCTCCCTCCAGCATCATGGCCAG43.278688524590164No Hit
GGATTCCGATGACCTCACAGCTCAAGTACGCCTGGGCACCAGTGACATTC43.278688524590164No Hit
TATCAACGCAGAGTACATGGGCTGCCCACCTCACCCTCAGCTCTGGCCTC32.459016393442623No Hit
GGCATCATTTGACTTTTGCTCGGAGATCTGAGCCGCCGTGTCCACCGCGG32.459016393442623No Hit
TCCCCACAGAAACCAGCCCAGGGCTGCAGGAAGGACAGGGGGGCCTCCAA32.459016393442623No Hit
GCTCCATCCCAGCTATCCTGTTCTTGCCCCGCAAGCGCTCTCAGGCAGAG32.459016393442623No Hit
CACTGTTTCCACAAAAATACAAACATTAGCTGGGCGTGGTGGCATGCACC21.639344262295082No Hit
ATCGAACCCTGATTCCCCGTCACCCGTGGTCACCATGGTAGGCACGGCGA21.639344262295082No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT21.639344262295082No Hit
CCCAAGATCCAACTACGAGCTTTTTAACTGCAGCAACTTTAATATACGCT21.639344262295082No Hit
GACCTGGCCTGCTGTGGGCAGCTCAGCCATGCAGGGTAGAGCTGGGAATG21.639344262295082No Hit
GACACAGCGAGCGGAGCCGCGGTCCGGACGGCAGCGCGTGCCCCGAGCTC21.639344262295082No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC21.639344262295082No Hit
ATCAACGCAGAGTACATGGGACATCTACCAAGTGGGCGGAGTGGCCTTCT21.639344262295082No Hit
CTTGATGAAGGCCCAGCCTGAGCCAATCAGGGCGATGGTGATGAAGAGGA21.639344262295082No Hit
GGTCTCCAGGGCTCCAGGCTGCTCACTGGGCTATGGCCCTTTAGGGGTCT21.639344262295082No Hit
GGGGTGTACTGGGGAGCCGTATCAGGTCCAGGGTCCTGATGATTCTCCTT21.639344262295082No Hit
CAATAGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGGG21.639344262295082No Hit
CGGTTTGATGATCCAGGTTTGATGCTCATGGGTTTCAAGCCGTTGGTACT21.639344262295082No Hit
CTCTCAGGCAGAGCTATGTGCAGACCCAAAGGAGCTCTGGGTGCAGCAGC21.639344262295082No Hit
CTTGTGATGTAATTATTATACGAATGGGGGCTTCAATCGGGAGTACTACT10.819672131147541No Hit
CCCAAAGCCAGAAGCTGGTGGGCATGAGAATGGCCAACCAGGCTCTGGGC10.819672131147541No Hit
CCTGTAAACATCGTAAATCTGCTCTTTGAAACATTTAATCAACATTTCAT10.819672131147541No Hit
CAGTAAAATAGGGTGTTGATCCCTGTATTCATGTCTCTCAAACATAGAGA10.819672131147541No Hit
GAGGCACGCACTCCTCAAGTGCAAAGTGTGAACGACAACACCAAATTTCC10.819672131147541No Hit
AACCAGCCCAGGGCTGCAGGAAGGACAGGGGGGCCTCCAAGACTGGCAAG10.819672131147541No Hit
GTATCAACGCAGAGTACATGGGACAGACCCCCAACTTGCAGCTGCCCACC10.819672131147541No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG10.819672131147541No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT10.819672131147541No Hit
GTCCATGACCGCGACCCCTCGGCGGGGGACACCCGCCCCCGCTCTCCGCA10.819672131147541No Hit
GGACTGCGCAGACCAGACTTCGCTCGTACTCGTGCGCCTCGCTTCGCTTT10.819672131147541No Hit
AGTAAAATCTGTCGAAATGCACCATGAAGCTTTGAGTGAAGCTCTTCCTG10.819672131147541No Hit
CCGCGTGCCCGCCGGGGGTCAGTGTCTTCCCGCAGCGCTCGCAGCGCAGG10.819672131147541No Hit
ATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGGACGCC10.819672131147541No Hit
CCTGTATACATCGTAAAGCTGATCTTTGAAACCTTTATTCAACATTTCAT10.819672131147541No Hit
AATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTG10.819672131147541No Hit
CCTTAGAGCTGCCCCTCTCTGGCCTGGCCGGCAGGCGTCTTCTTAACTCC10.819672131147541No Hit
GTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACACGCTGAGC10.819672131147541No Hit
GAAGACTATAAGCCCCAGCGGGCGACGACCGAACGCCCCCGGGAACACCG10.819672131147541No Hit
AAACATTCACTTATCAGACTAATTCAGCAAAGCATACACGTTCTTAGGTT10.819672131147541No Hit
GACGCCGACCGCTCGGGGGTCGCGTAACTAGTTAGCATGCCAGAGTCTCG10.819672131147541No Hit
CATTTGCCAAGAATGTTTTCATTAATCAAGAACGAAAGTCGGAGGTTCGA10.819672131147541No Hit
CTCCAGGGCCCCTGAGCATAGCCTCCTTCTTGCATCTTGGGTTCAGGCTT10.819672131147541No Hit
CAAGTGTTTTGTGCGAGTAGAAAACGTTAAAGGGGCAGTATGGACAGTGG10.819672131147541No Hit
CTCTCCGGCCCCGGCCGGGGGGCGGGCGCCGGCGGCTTTGGTGACTCTAG10.819672131147541No Hit
GACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAA10.819672131147541No Hit
ATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGC10.819672131147541No Hit
GTTTAGAGGGAGATCCTCCGAGTCTACCACCCCTCTAATGAAGTTCAGAT10.819672131147541No Hit
CAATCCACCTCATCACTTTCCTTGCTCTCTCTCTGTCACCTCATCTATCA10.819672131147541No Hit
GACTGAAGGGGAGCAAGAACCATGTTGCTCAGGGAGGCTGAGGCCGCCAC10.819672131147541No Hit
ATCCCATACTGGTCACAGAGCCAAAGTAACACAGTACTGCTTTCTTCTTA10.819672131147541No Hit
GGCCTGGAGGAGGCAGCAGGGGTCGAAGTGACCGGGGCAGTGGCCAGGGA10.819672131147541No Hit
CTGTAGCCTTGAACTCGTGGGCTCAAGCGATCCTGTCATCTCAGCCTCCC10.819672131147541No Hit
CCCTCTACCACAGACATGGCTCAGTCACTGGCTCTGAGCCTCCTTATCCT10.819672131147541No Hit
TCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGGCGTTATT10.819672131147541No Hit
GGCCAGCATGGGGTGGCTGCTCCAGGGCCCCTGAGCATAGCCTCCTTCTT10.819672131147541No Hit
GGGGGGAATTCAGATAAAACGAATAGCTCGTAACCAAACATGCACAGCGG10.819672131147541No Hit
GCTCAGGACTGTTGCCTCAAGTACAGCCAAAGGAAGATTCCCACCAAGGT10.819672131147541No Hit
GGACGGTTCTCACCCCCTCCAGTGGATGTATGGCTGCGACCTGGGGCCCG10.819672131147541No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCTCGATGCTCTTAGCTGAGTGTC10.819672131147541No Hit
AGCTTAGGCTGCTCCATCCCAGCTATCCTGTTCTTGCCCCGCTAGCGCTC10.819672131147541No Hit
GGACTGTTGCCTCAAGTACAGCCAAAGGAAGATTCCCGCCAAGGTTGTCC10.819672131147541No Hit
ATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCGAATGGCTCA10.819672131147541No Hit
CTCTACCACAGACATGGCTCAGTCACTGGCTCTGAGCCTCCTTATCCTGG10.819672131147541No Hit
GCTCAGAGGTGCTTTGAAGATGCCGGAGGCCCCGCCTCTGCTGTTGGCAG10.819672131147541No Hit
GAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCAC10.819672131147541No Hit
GCATGTAGTAGAAGGGGAACCATGACAGGAAGATATCAGAGAAGAATTCA10.819672131147541No Hit
GCAGGGTAGAGCTGGGAATGCAGATGGGGTGGTTAAAGCAGGAGAAAGAG10.819672131147541No Hit
ACGCAGAGTACATGGGACAGACCCCCAACTTGCAGCTGCCCACCTCACCC10.819672131147541No Hit
CGGTTTGATGATCCAGGTGTGATGCTCATGGGTTTCAAGCCGTTGGTACT10.819672131147541No Hit
AGCCCGAGGAGCCTCCTGAAGGTGGAAGCTATGCTGGTTCCCTGGCTGAG10.819672131147541No Hit
TCATAGACGAGCTCTTCCAGGACAGGTTCTTCACCCGGGAGCCCCAGGAT10.819672131147541No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACC10.819672131147541No Hit
TAAGTACGCACGGCCGGTACAGTGAAACTGCGAATGGCTCATTAAATCAG10.819672131147541No Hit
CCCATAGTAGGCCTAAAAGCAGTCACCAATTAAGAAAGCGTTCAAGCTCA10.819672131147541No Hit
GATTACAGGTGTGAACCACTATGCCCGGCCAATACAATCTGTTTAAAACT10.819672131147541No Hit
GTGCTTATCCTTCTCTCGCAAACGTTCCAGTTTGGCAGCCATTTGTGCCT10.819672131147541No Hit
CTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCATGGTAGGC10.819672131147541No Hit
GGGCAGCACAGACCTGTTATTGATCAATATCGGGTGGCTGAACGCCACTT10.819672131147541No Hit
TCGCCGTGGAGTACGTAGACGACACCCAGTTCGTGAGGTTCGACAGCGAC10.819672131147541No Hit
GTCACAGACCCCTAAAGGGCCATAGCCCAGTGAGCAGCCTGGAGCCCTGG10.819672131147541No Hit
GAACTGGCTCAGGAAGGAGGCAGGAATGAGCTGACCGAGACAAGGCATCC10.819672131147541No Hit
CCTCGGGGAGACTCAAGCCAGATGCCAGCCACGGGGACCACAGCCTTGGC10.819672131147541No Hit
ATTCTAAGCAGAGTATGTAAATTGGAAGTTAACTTATGCACGCTTAACTA10.819672131147541No Hit
GAGGTGGGGTGTACTGGGGAGCCGTATCAGGTCCAGGGTCCTGATGATTC10.819672131147541No Hit
CTGCAGCCCTGGGCTGGTTTCTGTGGGGATGGTGTCTTGTCCAGATGCTG10.819672131147541No Hit
GTCCAGGCTCCCACACAGTCACACAGTCATACACACAGCGTTAAGGCGTC10.819672131147541No Hit
ATGCAATATATGGCTCTACAATCCTCAGCATGTTAATCGAAGCCTTGTTG10.819672131147541No Hit
AGGATACTGCGAGCAAATGGGGTGGAGGGGTGCTCTCCTGAGCTACAGAA10.819672131147541No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)