FastQCFastQC Report
Tue 10 May 2022
EGAF00003650323

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003650323
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11732
Sequences flagged as poor quality0
Sequence length146
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC310.26423457211046714No Hit
TCCATATAGTCACTCCAGGTTTATGGAGGGTTCTTCTACTATTAGGACTT310.26423457211046714No Hit
GGTGTAGCCTGAGAATAGGGGAAATCAGTGAATGAAGCCTCCTATGATGG300.2557108762359359No Hit
GGTCTAGGGTGTAGCCTGAGAATAGGGGAAATCAGTGAATGAAGCCTCCT290.2471871803614047No Hit
ACTACTAACAGACCGCAACCTCAACACCACCTTCTTCGACCCCGCCGGAG280.23866348448687352No Hit
GGTGTATGCATCGGGGTAGTCCGAGTAACGTCGGGGCATTCCGGATAGGC250.21309239686327994No Hit
TCACTATACTACTAACAGACCGCAACCTCAACACCACCTTCTTCGACCCC230.19604500511421755No Hit
CACCATATATTTACAGTAGGAATAGACGTAGACACACGAGCATATTTCAC230.19604500511421755No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC220.18752130923968632No Hit
CCGTAGACCTAACCATCTTCTCCTTACACCTAGCAGGTGTCTCCTCTATC210.17899761336515513No Hit
ACCCAATACCAAACGCCCCTCTTCGTCTGATCCGTCCTAATCACAGCAGT210.17899761336515513No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT210.17899761336515513No Hit
CCTATTGATAGGACATAGTGGAAGTGAGCTACAACGTAGTACGTGTCGTG210.17899761336515513No Hit
GGTATGGTCTGAGCTATGATATCAATTGGCTTCCTAGGGTTTATCGTGTG210.17899761336515513No Hit
CTCCCAGTCCTAGCTGCTGGCATCACTATACTACTAACAGACCGCAACCT200.17047391749062393No Hit
GGCAAATACAGCTCCTATTGATAGGACATAGTGGAAGTGAGCTACAACGT200.17047391749062393No Hit
ACGTAGTACGTGTCGTGTAGTACGATGTCTAGTGATGAGTTTGCTAATAC190.16195022161609274No Hit
GACCATACCTATGTATCCAAATGGTTCTTTTTTTCCGGAGTAGTAAGTTA190.16195022161609274No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT190.16195022161609274No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA190.16195022161609274No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA190.16195022161609274No Hit
GAATATGATAGTGAAATGGATTTTGGCGTAGGTTTGGTCTAGGGTGTAGC180.15342652574156154No Hit
GATTATGGTAGCGGAGGTGAAATATGCTCGTGTGTCTACGTCTATTCCTA180.15342652574156154No Hit
CTCCTACTCCTGCTCGCATCTGCTATAGTGGAGGCCGGAGCAGGAACAGG180.15342652574156154No Hit
CATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAATG180.15342652574156154No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG180.15342652574156154No Hit
GCCATAACCCAATACCAAACGCCCCTCTTCGTCTGATCCGTCCTAATCAC180.15342652574156154No Hit
GGACTATTCCTAGCCATGCACTACTCACCAGACGCCTCAACCGCCTTTTC170.14490282986703035No Hit
GTCTAGTGATGAGTTTGCTAATACAATGCCAGTCAGGCCACCTACGGTGA170.14490282986703035No Hit
TATATGGTGTGCTCACACGATAAACCCTAGGAAGCCAATTGATATCATAG170.14490282986703035No Hit
TAGTGGAAGTGAGCTACAACGTAGTACGTGTCGTGTAGTACGATGTCTAG160.13637913399249915No Hit
GTCCTAATCACAGCAGTCCTACTTCTCCTATCTCTCCCAGTCCTAGCTGC160.13637913399249915No Hit
CATTGGGACAGACCTAGTTCAATGAATCTGAGGAGGCTACTCAGTAGACA160.13637913399249915No Hit
GCTCATGAGTGCAAGACGTCTTGTGATGTAATTATTATACGAATGGGGGC160.13637913399249915No Hit
TGCTAATACAATGCCAGTCAGGCCACCTACGGTGAAAAGAAAGATGAATC160.13637913399249915No Hit
GCTCAGACCATACCTATGTATCCAAATGGTTCTTTTTTTCCGGAGTAGTA160.13637913399249915No Hit
CCCCACCCCATCCAACATCTCCGCATGATGAAACTTCGGCTCACTCCTTG160.13637913399249915No Hit
GCTTACTGGTTGTCCTCCGATTCAGGTTAGAATGAGGAGGTCTGCGGCTA160.13637913399249915No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG150.12785543811796796No Hit
GAATAGGGGAAATCAGTGAATGAAGCCTCCTATGATGGCAAATACAGCTC150.12785543811796796No Hit
GTACATGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTC150.12785543811796796No Hit
GTATTAGGATTAGGATTGTTGTGAAGTATAGTACGGATGCTACTTGTCCA150.12785543811796796No Hit
GTCCCAATGTATGGGATGGCGGATAGTAAGTTTGTAATTACTGTGGCCCC150.12785543811796796No Hit
ATCTTAGGGGCCATCAATTTCATCACAACAATTATCAATATAAAACCCCC140.11933174224343676No Hit
CCTCTATATAAATGCGTAGGGGTTTTAGTTAAATGTCCTTTGAAGTATAC140.11933174224343676No Hit
ATTCCTAGCCATGCACTACTCACCAGACGCCTCAACCGCCTTTTCATCAA140.11933174224343676No Hit
CGTCTGATCCGTCCTAATCACAGCAGTCCTACTTCTCCTATCTCTCCCAG140.11933174224343676No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA140.11933174224343676No Hit
ACTTACTACTCCGGAAAAAAAGAACCATTTGGATACATAGGTATGGTCTG140.11933174224343676No Hit
GAGCTACAACGTAGTACGTGTCGTGTAGTACGATGTCTAGTGATGAGTTT140.11933174224343676No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA140.11933174224343676No Hit
GAGTAACGTCGGGGCATTCCGGATAGGCCGAGAAAGTGTTGTGGGAAGAA130.11080804636890555No Hit
GTCTACGTCTATTCCTACTGTAAATATATGGTGTGCTCACACGATAAACC130.11080804636890555No Hit
GTCCAGTCATGACCTCGATCCATCGCGTATAGGGACGCCCCCTGCTCGCG130.11080804636890555No Hit
GATTATAACAACGGTGCAATCTCAGCTAAACCGACGCAGTTTTGCTCCTT130.11080804636890555No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT130.11080804636890555No Hit
CTATAGTGGAGGCCGGAGCAGGAACAGGTTGAACAGTCTACCCTCCCTTA130.11080804636890555No Hit
TGTGGGGACAGCTCATGAGTGCAAGACGTCTTGTGATGTAATTATTATAC130.11080804636890555No Hit
GCTCTGAGCCCTAGGATTCATCTTTCTTTTCACCGTAGGTGGCCTGACTG130.11080804636890555No Hit
TCCGTGGAGTGTGGCGAGTCAGCTAAATACTTTGACGCCGGTGGGGATAG130.11080804636890555No Hit
TATATTTACAGTAGGAATAGACGTAGACACACGAGCATATTTCACCTCCG130.11080804636890555No Hit
GAACTACTCCCACCCTGGAGCCTCCGTAGACCTAACCATCTTCTCCTTAC130.11080804636890555No Hit
GCCATGCACTACTCACCAGACGCCTCAACCGCCTTTTCATCAATCGCCCA130.11080804636890555No Hit
CGTCGGGGCATTCCGGATAGGCCGAGAAAGTGTTGTGGGAAGAAAGTTAG130.11080804636890555No Hit
CATCGGGGTAGTCCGAGTAACGTCGGGGCATTCCGGATAGGCCGAGAAAG130.11080804636890555No Hit
TATTTCACCTCCGCTACCATAATCATCGCTATCCCCACCGGCGTCAAAGT120.10228435049437436No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC120.10228435049437436No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG120.10228435049437436No Hit
AGCATGTGTAGTGCGCCATATCATATCCAAGATAGTGTAGGACTCGTCGC120.10228435049437436No Hit
GTCCCACCCTCACACGATTCTTTACCTTTCACTTCATCTTGCCCTTCATT120.10228435049437436No Hit
CTCCACGGAAGCAATATGAAATGATCTGCTGCAGTGCTCTGAGCCCTAGG120.10228435049437436No Hit
GCTATAGGGTAAATACGGGTCCTATTTCAAAGATTTTTAGGGGAATTAAT120.10228435049437436No Hit
CCTCTATCTTAGGGGCCATCAATTTCATCACAACAATTATCAATATAAAA120.10228435049437436No Hit
GGTGGAAGGTGATTTTATCGGAATGGGAGGTGATTCCTAGGGGGTTGTTT120.10228435049437436No Hit
GGCTATGTCCTCCCGTGAGGCCAAATATCATTCTGAGGGGCCACAGTAAT120.10228435049437436No Hit
GTCCTAGCTGCTGGCATCACTATACTACTAACAGACCGCAACCTCAACAC120.10228435049437436No Hit
CCCATACATTGGGACAGACCTAGTTCAATGAATCTGAGGAGGCTACTCAG120.10228435049437436No Hit
GTGTAGTACGATGTCTAGTGATGAGTTTGCTAATACAATGCCAGTCAGGC120.10228435049437436No Hit
CGATAAACCCTAGGAAGCCAATTGATATCATAGCTCAGACCATACCTATG120.10228435049437436No Hit
ATGTCCTCCCGTGAGGCCAAATATCATTCTGAGGGGCCACAGTAATTACA120.10228435049437436No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)