FastQCFastQC Report
Tue 10 May 2022
EGAF00003650324

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003650324
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11732
Sequences flagged as poor quality0
Sequence length146
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATATAGTCACTCCAGGTTTATGGAGGGTTCTTCTACTATTAGGACTT450.38356631435390387No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC310.26423457211046714No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT310.26423457211046714No Hit
GGTCTAGGGTGTAGCCTGAGAATAGGGGAAATCAGTGAATGAAGCCTCCT290.2471871803614047No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT250.21309239686327994No Hit
CCCCACCCCATCCAACATCTCCGCATGATGAAACTTCGGCTCACTCCTTG240.20456870098874871No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA240.20456870098874871No Hit
GACCATACCTATGTATCCAAATGGTTCTTTTTTTCCGGAGTAGTAAGTTA230.19604500511421755No Hit
GTACATGGGATGACCCCAATACGCAAAATTAACCCCCTAATAAAATTAAT230.19604500511421755No Hit
GTCCTAATCACAGCAGTCCTACTTCTCCTATCTCTCCCAGTCCTAGCTGC220.18752130923968632No Hit
CACCATATATTTACAGTAGGAATAGACGTAGACACACGAGCATATTTCAC210.17899761336515513No Hit
TGCTAATACAATGCCAGTCAGGCCACCTACGGTGAAAAGAAAGATGAATC200.17047391749062393No Hit
TATATGGTGTGCTCACACGATAAACCCTAGGAAGCCAATTGATATCATAG200.17047391749062393No Hit
GCCATAACCCAATACCAAACGCCCCTCTTCGTCTGATCCGTCCTAATCAC200.17047391749062393No Hit
GGACTATTCCTAGCCATGCACTACTCACCAGACGCCTCAACCGCCTTTTC190.16195022161609274No Hit
ACGTAGTACGTGTCGTGTAGTACGATGTCTAGTGATGAGTTTGCTAATAC190.16195022161609274No Hit
GTATTAGGATTAGGATTGTTGTGAAGTATAGTACGGATGCTACTTGTCCA190.16195022161609274No Hit
GGGGTAGTCCGAGTAACGTCGGGGCATTCCGGATAGGCCGAGAAAGTGTT190.16195022161609274No Hit
GGTGTATGCATCGGGGTAGTCCGAGTAACGTCGGGGCATTCCGGATAGGC190.16195022161609274No Hit
CCCTAGACCAAACCTACGCCAAAATCCATTTCACTATCATATTCATCGGC190.16195022161609274No Hit
GGTATGGTCTGAGCTATGATATCAATTGGCTTCCTAGGGTTTATCGTGTG190.16195022161609274No Hit
GCTATAGGGTAAATACGGGTCCTATTTCAAAGATTTTTAGGGGAATTAAT180.15342652574156154No Hit
GTGTAGTACGATGTCTAGTGATGAGTTTGCTAATACAATGCCAGTCAGGC180.15342652574156154No Hit
GGTGGGGGGCATCCATATAGTCACTCCAGGTTTATGGAGGGTTCTTCTAC180.15342652574156154No Hit
CCTTACACCTAGCAGGTGTCTCCTCTATCTTAGGGGCCATCAATTTCATC170.14490282986703035No Hit
GTCTAGTGATGAGTTTGCTAATACAATGCCAGTCAGGCCACCTACGGTGA170.14490282986703035No Hit
GCCTACAGATGATAGGATGTTTCATGTGGTGTATGCATCGGGGTAGTCCG170.14490282986703035No Hit
GGTCTGAGCTATGATATCAATTGGCTTCCTAGGGTTTATCGTGTGAGCAC160.13637913399249915No Hit
ATCTTAGGGGCCATCAATTTCATCACAACAATTATCAATATAAAACCCCC160.13637913399249915No Hit
ACTACTAACAGACCGCAACCTCAACACCACCTTCTTCGACCCCGCCGGAG160.13637913399249915No Hit
ATTCCTAGCCATGCACTACTCACCAGACGCCTCAACCGCCTTTTCATCAA160.13637913399249915No Hit
GGCTATGTCCTCCCGTGAGGCCAAATATCATTCTGAGGGGCCACAGTAAT160.13637913399249915No Hit
CATCGGGGTAGTCCGAGTAACGTCGGGGCATTCCGGATAGGCCGAGAAAG160.13637913399249915No Hit
GCTCTAAGCCTCCTTATTCGAGCCGAGCTGGGCCAGCCAGGCAACCTTCT150.12785543811796796No Hit
CATTGGGACAGACCTAGTTCAATGAATCTGAGGAGGCTACTCAGTAGACA150.12785543811796796No Hit
GAACTACTCCCACCCTGGAGCCTCCGTAGACCTAACCATCTTCTCCTTAC150.12785543811796796No Hit
TCACTATACTACTAACAGACCGCAACCTCAACACCACCTTCTTCGACCCC150.12785543811796796No Hit
TCCTAGTCCTGTATGCCCTTTTCCTAACACTCACAACAAAACTAACTAAT150.12785543811796796No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC150.12785543811796796No Hit
TCCTAGGGTTTATCGTGTGAGCACACCATATATTTACAGTAGGAATAGAC140.11933174224343676No Hit
GAATAGGGGAAATCAGTGAATGAAGCCTCCTATGATGGCAAATACAGCTC140.11933174224343676No Hit
ATTCTATACCAACACCTATTCTGATTTTTCGGTCACCCTGAAGTTTATAT140.11933174224343676No Hit
CCCCTATCCCCTACGAGGCTACCCGGATCGATGACGCGAATTGGGGACAT140.11933174224343676No Hit
TGTGGGGACAGCTCATGAGTGCAAGACGTCTTGTGATGTAATTATTATAC140.11933174224343676No Hit
GGTGTAGCCTGAGAATAGGGGAAATCAGTGAATGAAGCCTCCTATGATGG140.11933174224343676No Hit
ATATAAATGCGTAGGGGTTTTAGTTAAATGTCCTTTGAAGTATACTTGAG140.11933174224343676No Hit
TCTCCTATCTCTCCCAGTCCTAGCTGCTGGCATCACTATACTACTAACAG140.11933174224343676No Hit
ATATAAAACCCCCTGCCATAACCCAATACCAAACGCCCCTCTTCGTCTGA140.11933174224343676No Hit
CCTAGGAGGTCTGGTGAGAATAGTGTTAATGTCATTAAGGAGAGAAGGAA130.11080804636890555No Hit
GTATAATTGTCTGGGTCGCCTAGGAGGTCTGGTGAGAATAGTGTTAATGT130.11080804636890555No Hit
GTATTAGCAAACTCATCACTAGACATCGTACTACACGACACGTACTACGT130.11080804636890555No Hit
GGGTAAATACGGGTCCTATTTCAAAGATTTTTAGGGGAATTAATTCTAGG130.11080804636890555No Hit
CTCCTAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGC130.11080804636890555No Hit
GAATATGATAGTGAAATGGATTTTGGCGTAGGTTTGGTCTAGGGTGTAGC130.11080804636890555No Hit
CCGTAGACCTAACCATCTTCTCCTTACACCTAGCAGGTGTCTCCTCTATC130.11080804636890555No Hit
GTTGTGAAGTATAGTACGGATGCTACTTGTCCAATGATGGTAAAAGGGTA130.11080804636890555No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA130.11080804636890555No Hit
CTCCTACTCCTGCTCGCATCTGCTATAGTGGAGGCCGGAGCAGGAACAGG130.11080804636890555No Hit
TCATAGCTCAGACCATACCTATGTATCCAAATGGTTCTTTTTTTCCGGAG130.11080804636890555No Hit
TAGTGGAAGTGAGCTACAACGTAGTACGTGTCGTGTAGTACGATGTCTAG120.10228435049437436No Hit
GATGTGGGGAGGGGTGTTTAAGGGGTTGGCTAGGGTATAATTGTCTGGGT120.10228435049437436No Hit
GTCTACGTCTATTCCTACTGTAAATATATGGTGTGCTCACACGATAAACC120.10228435049437436No Hit
GTTTAAGGGGTTGGCTAGGGTATAATTGTCTGGGTCGCCTAGGAGGTCTG120.10228435049437436No Hit
TCACTAGACATCGTACTACACGACACGTACTACGTTGTAGCTCACTTCCA120.10228435049437436No Hit
GGCAAATACAGCTCCTATTGATAGGACATAGTGGAAGTGAGCTACAACGT120.10228435049437436No Hit
CCCCAATACGCAAAATTAACCCCCTAATAAAATTAATTAACCACTCATTC120.10228435049437436No Hit
CCCTTAGACGTCTTGGTATACGGACAACTGATGGACCCACGTTGCGAGTC120.10228435049437436No Hit
ATATAGATTGGCGCTAGTAGATCACAGAACAACCGCCGCATACGGCCGAT120.10228435049437436No Hit
GCTAAATACTTTGACGCCGGTGGGGATAGCGATGATTATGGTAGCGGAGG120.10228435049437436No Hit
TTTCAGAGCATTGACCGTAGTATACCCCCGGTCGTGTAGCGGTGAAAGTG120.10228435049437436No Hit
GCCATGCACTACTCACCAGACGCCTCAACCGCCTTTTCATCAATCGCCCA120.10228435049437436No Hit
CCCTGAGACAGAGTCGCTATCGTTATGTCTCCTTCCCGCGGTCAAGGCGA120.10228435049437436No Hit
GGGTATAATTGTCTGGGTCGCCTAGGAGGTCTGGTGAGAATAGTGTTAAT120.10228435049437436No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)