FastQCFastQC Report
Tue 7 May 2024
EGAF00003650325

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003650325
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11586
Total Bases1.6 Mbp
Sequences flagged as poor quality0
Sequence length146
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC410.3538753668220266No Hit
GGTCTAGGGTGTAGCCTGAGAATAGGGGAAATCAGTGAATGAAGCCTCCT370.3193509407906094No Hit
TCCATATAGTCACTCCAGGTTTATGGAGGGTTCTTCTACTATTAGGACTT320.2761954082513378No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA300.25893319523562924No Hit
GGTATGGTCTGAGCTATGATATCAATTGGCTTCCTAGGGTTTATCGTGTG290.2503020887277749No Hit
ACGTAGTACGTGTCGTGTAGTACGATGTCTAGTGATGAGTTTGCTAATAC250.21577766269635767No Hit
GACCATACCTATGTATCCAAATGGTTCTTTTTTTCCGGAGTAGTAAGTTA230.19851544968064908No Hit
TATATGGTGTGCTCACACGATAAACCCTAGGAAGCCAATTGATATCATAG220.18988434317279476No Hit
GTGTAGTACGATGTCTAGTGATGAGTTTGCTAATACAATGCCAGTCAGGC210.18125323666494045No Hit
CCTATTGATAGGACATAGTGGAAGTGAGCTACAACGTAGTACGTGTCGTG210.18125323666494045No Hit
CCCCACCCCATCCAACATCTCCGCATGATGAAACTTCGGCTCACTCCTTG200.17262213015708613No Hit
CACCATATATTTACAGTAGGAATAGACGTAGACACACGAGCATATTTCAC200.17262213015708613No Hit
ATTCTATACCAACACCTATTCTGATTTTTCGGTCACCCTGAAGTTTATAT190.16399102364923185No Hit
GCTATAGGGTAAATACGGGTCCTATTTCAAAGATTTTTAGGGGAATTAAT190.16399102364923185No Hit
GTATAGTACGGATGCTACTTGTCCAATGATGGTAAAAGGGTAGCTTACTG190.16399102364923185No Hit
GGGTATAATTGTCTGGGTCGCCTAGGAGGTCTGGTGAGAATAGTGTTAAT190.16399102364923185No Hit
GCTCTAAGCCTCCTTATTCGAGCCGAGCTGGGCCAGCCAGGCAACCTTCT180.15535991714137753No Hit
ATTCCTAGCCATGCACTACTCACCAGACGCCTCAACCGCCTTTTCATCAA180.15535991714137753No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT180.15535991714137753No Hit
GAATATGATAGTGAAATGGATTTTGGCGTAGGTTTGGTCTAGGGTGTAGC180.15535991714137753No Hit
GAGTAATATAGATTGGCGCTAGTAGATCACAGAACAACCGCCGCATACGG180.15535991714137753No Hit
GGCAAATACAGCTCCTATTGATAGGACATAGTGGAAGTGAGCTACAACGT180.15535991714137753No Hit
CCCTGAAGTTTATATTCTTATCCTACCAGGCTTCGGAATAATCTCCCATA180.15535991714137753No Hit
GTACATGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTC170.14672881063352322No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG170.14672881063352322No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT170.14672881063352322No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA170.14672881063352322No Hit
GGACTATTCCTAGCCATGCACTACTCACCAGACGCCTCAACCGCCTTTTC160.1380977041256689No Hit
ACCATAATCATCGCTATCCCCACCGGCGTCAAAGTATTTAGCTGACTCGC160.1380977041256689No Hit
CCCCTATCCCCTACGAGGCTACCCGGATCGATGACGCGAATTGGGGACAT160.1380977041256689No Hit
GCTCAGACCATACCTATGTATCCAAATGGTTCTTTTTTTCCGGAGTAGTA160.1380977041256689No Hit
GGTTTATGGAGGGTTCTTCTACTATTAGGACTTTTCGCTTCGAAGCGAAG160.1380977041256689No Hit
GCTCAGAGCACTGCAGCAGATCATTTCATATTGCTTCCGTGGAGTGTGGC160.1380977041256689No Hit
GTCCCAATGTATGGGATGGCGGATAGTAAGTTTGTAATTACTGTGGCCCC160.1380977041256689No Hit
CATCTACAACGTTATCGTCACAGCCCATGCATTTGTAATAATCTTCTTCA160.1380977041256689No Hit
GGCCTATATTACGGATCATTTCTCTACTCAGAAACCTGAAACATCGGCAT150.12946659761781462No Hit
GTATTAGCAAACTCATCACTAGACATCGTACTACACGACACGTACTACGT150.12946659761781462No Hit
CCCCTATTCTCAGGCTACACCCTAGACCAAACCTACGCCAAAATCCATTT150.12946659761781462No Hit
CCTTACACCTAGCAGGTGTCTCCTCTATCTTAGGGGCCATCAATTTCATC150.12946659761781462No Hit
GCCATGCACTACTCACCAGACGCCTCAACCGCCTTTTCATCAATCGCCCA150.12946659761781462No Hit
TAGTGGAAGTGAGCTACAACGTAGTACGTGTCGTGTAGTACGATGTCTAG140.1208354911099603No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG140.1208354911099603No Hit
GTACATGGGCTGATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAA140.1208354911099603No Hit
ACTACTAACAGACCGCAACCTCAACACCACCTTCTTCGACCCCGCCGGAG140.1208354911099603No Hit
GGTGAGAATAGTGTTAATGTCATTAAGGAGAGAAGGAAGAGAAGTAAGCC140.1208354911099603No Hit
TGCTAATACAATGCCAGTCAGGCCACCTACGGTGAAAAGAAAGATGAATC140.1208354911099603No Hit
GTACATGGGATGACCCCAATACGCAAAATTAACCCCCTAATAAAATTAAT140.1208354911099603No Hit
TCATAGAAGAGCTTATCACCTTTCATGATCACGCCCTCATAATCATTTTC140.1208354911099603No Hit
GGGGTAGTCCGAGTAACGTCGGGGCATTCCGGATAGGCCGAGAAAGTGTT140.1208354911099603No Hit
GGTGTATGCATCGGGGTAGTCCGAGTAACGTCGGGGCATTCCGGATAGGC140.1208354911099603No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT140.1208354911099603No Hit
GGATGACCCCAATACGCAAAATTAACCCCCTAATAAAATTAATTAACCAC140.1208354911099603No Hit
GGCTAGGGTATAATTGTCTGGGTCGCCTAGGAGGTCTGGTGAGAATAGTG140.1208354911099603No Hit
GGGTAGAGGGGGTGCTATAGGGTAAATACGGGTCCTATTTCAAAGATTTT140.1208354911099603No Hit
TCATAGCTCAGACCATACCTATGTATCCAAATGGTTCTTTTTTTCCGGAG140.1208354911099603No Hit
CATTGGGACAGACCTAGTTCAATGAATCTGAGGAGGCTACTCAGTAGACA130.112204384602106No Hit
CCATCATCCTAGTCCTCATCGCCCTCCCATCCCTACGCATCCTTTACATA130.112204384602106No Hit
GTCCCACCCTCACACGATTCTTTACCTTTCACTTCATCTTGCCCTTCATT130.112204384602106No Hit
ATATAAATGCGTAGGGGTTTTAGTTAAATGTCCTTTGAAGTATACTTGAG130.112204384602106No Hit
GGTGAAATATGCTCGTGTGTCTACGTCTATTCCTACTGTAAATATATGGT130.112204384602106No Hit
GCCTACAGATGATAGGATGTTTCATGTGGTGTATGCATCGGGGTAGTCCG130.112204384602106No Hit
CCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTTGA130.112204384602106No Hit
ACTTACTACTCCGGAAAAAAAGAACCATTTGGATACATAGGTATGGTCTG130.112204384602106No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC130.112204384602106No Hit
CCCTAGACCAAACCTACGCCAAAATCCATTTCACTATCATATTCATCGGC130.112204384602106No Hit
GGTGTACACCGCCCTTAGACGTCTTGGTATACGGACAACTGATGGACCCA130.112204384602106No Hit
ATTTCATATTGCTTCCGTGGAGTGTGGCGAGTCAGCTAAATACTTTGACG120.10357327809425168No Hit
GGGTAAATACGGGTCCTATTTCAAAGATTTTTAGGGGAATTAATTCTAGG120.10357327809425168No Hit
GATTAGGACGGATCAGACGAAGAGGGGCGTTTGGTATTGGGTTATGGCAG120.10357327809425168No Hit
TTCATGTGGTGTATGCATCGGGGTAGTCCGAGTAACGTCGGGGCATTCCG120.10357327809425168No Hit
CTCCTACTCCTGCTCGCATCTGCTATAGTGGAGGCCGGAGCAGGAACAGG120.10357327809425168No Hit
GGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAG120.10357327809425168No Hit
GCCATAACCCAATACCAAACGCCCCTCTTCGTCTGATCCGTCCTAATCAC120.10357327809425168No Hit
CCAGACGCCTCAACCGCCTTTTCATCAATCGCCCACATCACTCGAGACGT120.10357327809425168No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)