FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003650351

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003650351
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences129
Sequences flagged as poor quality0
Sequence length146
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTATCGTTCGCCGCATTCATTTCAAATTCAAGCTTTTCTTTTACATAAT32.3255813953488373No Hit
TATTTCAGCTCGGTGCCGCCATACTTGGAGAAGATGACGGTGTCGCCCTC32.3255813953488373No Hit
GCGCTGCAGGCCCAGCAGCTGAGGCTTCACCGCCTGCCAGACATCGACAT32.3255813953488373No Hit
GTGTTAAAACCTGCAGATGAAGTCACTTCATACTTCTCTCAGACTTTCAT21.550387596899225No Hit
TCGCTGCAGAACATCCGCCTCGCAGTGGATAAGCTGCGCGACCGCGGTGT21.550387596899225No Hit
GGATTGTGAATCTGCTTCATACGCATTCATAAATGCATCGTGAATTTTGT21.550387596899225No Hit
ATGCCGTCCAGTTTCACGTTGCCGTTGTCGTACGCGGCACCCGGGTTACC21.550387596899225No Hit
GTTCTCTCTCTTCAATATTGCGATTTGTGCCCGTTCATCTTGCGTCAGGT21.550387596899225No Hit
GTCCTGATCGAATCAGGGAAAATAAAAGCAATCGGCACAAACCTCGATGC21.550387596899225No Hit
TTCTTCTACGGCCTCGAAGAGGGCGAGGAGACCATGATCTGGTACGGCGA21.550387596899225No Hit
TTTCAGCGGGAGGTTGCGTGATGGCTATGTACGAGGTGCCTGCGGGCACC21.550387596899225No Hit
ACCGTCTGCCGGTCTATAATTCATTCGGCATGACAGAGACGTGCTCACAG21.550387596899225No Hit
CGCTTAACTTGCTAGCATGATGCTAATTTCGTAGCATGGCGAAAATCCGT21.550387596899225No Hit
GCCAGGACGAAGCCGTGGATCTGGTTGCGGATGCCGTCATCCGTGCAAGA21.550387596899225No Hit
GCGCGACTGGGTGCAGGGCACGGATGGGCGTTGGGCGACGGTGGCGTTGC21.550387596899225No Hit
GAGCTTCATCATTACCTGCTTCACGTTCTTCCATTTGACTTTCGAAACGT21.550387596899225No Hit
CGTTGGAACGTGTGGTGACTGTACAATTTGTATTGACAGATGTCCAACAG21.550387596899225No Hit
CCGGACAGCCGGTCACGGTAGTGCAGGAAACGCTTCAGACCGCCGAGCTC21.550387596899225No Hit
AAACTAAATAAAGTTGAAGATAAAACTGCGCACTACGACGTTAAAACCTG21.550387596899225No Hit
TTCCATTATCAACCGCTACATGCGATTTGTGGATCAGGATGAGCATAAAC21.550387596899225No Hit
GTGATGAAGTTAATAGACGCGGTGGGGCGCCGGCCGTTGTGCTGGTACAG21.550387596899225No Hit
GCACTCCTCGCCGTGCGGGCAGCGGGTCGCGCCCAGGCACTCGTTGGAGG21.550387596899225No Hit
CGTATACGGACGGTGGATACATATCACACCCAGAATGAAATGAAACACCA21.550387596899225No Hit
CACGGGCATATTGGAAAAGTACGCCGGCGGGCCTTCCTGCTCGATCATCT10.7751937984496124No Hit
ACCTCGGTGAGGTCTCAATCCACTGCTTCGGTATCTTTTCACCGCGGGCC10.7751937984496124No Hit
TCATAATAGATTTTTGATTTTTTAATTCCGTTATGAATAATTAATTGATG10.7751937984496124No Hit
GTCTATGGGGACGGAATGGTTCCGGAAAGACGACATTGATGAAAATCTTA10.7751937984496124No Hit
GTATAGCGAAAGAGAAGTCTAAGGGTGACGAAGTTTTTGGAAGTACGATT10.7751937984496124No Hit
CATCCACCGACGGCGCCACCACGGCACCGACGCGCTCCGAGATCTCCTGA10.7751937984496124No Hit
CAGAAGCATTAGGGGGTATGATTGGTGCAGCATCAATTGCCTGTCTCTTA10.7751937984496124No Hit
GAAATAATAAAGTCCCAAATACCCCACAAGTACCGCCGTACAGGCGGCCA10.7751937984496124No Hit
CTGCAACAGACGCAATAGCCTTCTCACGCTGCGCTGCGAAGTTGGGATGC10.7751937984496124No Hit
CTTGTTGGTGTGACCCAAGGCTCGAATGGCGGCATGGGTTATGGCTACTT10.7751937984496124No Hit
GACCCAATCGGACTCGCCTGGTCCTAAGTTCGCTTCAGGGTCCGTCCCTG10.7751937984496124No Hit
ATGCCAAAGGTCATCGCTGCAATAGAGCGGCTCGAAAATATCAGGAGCGA10.7751937984496124No Hit
CTATAAGAAAGAGCATTCGCCGAACCGTGAAAATGAACTGCTTGAAAAAG10.7751937984496124No Hit
CTGCAGGCCGGGGTGCCGGAGATCGTCGAGTTGTACCGCAATGCCCAGGC10.7751937984496124No Hit
AGTGTGGACTTTGCCATCTCCTCACACGCGGCCAGATCCCCACCCTCCTG10.7751937984496124No Hit
GTGTCGATTCCCCGCGACACCTACATCCACGACGCCACCTACCTGTCTCT10.7751937984496124No Hit
ATTAACGGTCCGCCAAGAGCTCAATGGCTTCGGCCCACCTCGCGGTGTTC10.7751937984496124No Hit
CGTCTGATCCCGGGCGTGCTGGGCAACACCGAAAGCCACGAGGACGACAG10.7751937984496124No Hit
GCAGAGCCCGGCGCCAAAGACGCGGCTTGAAGGCGCAGGCGCAGCAGCAC10.7751937984496124No Hit
TGAAGGTACTGCTGAAGGTCACAATGGTGAATTAGTCGCAACAGTAACTT10.7751937984496124No Hit
TGAAGGTACTGCTGAAGGTCACAATGGTGAATTAGTAGCAACAGTAACTT10.7751937984496124No Hit
GAAACTTTCCCGCAGTCTACAGCTTGGAAACTCACAGTTTGGATTGTGAT10.7751937984496124No Hit
ATATTTTGCTGACCCCTATGCATCGTGGCAACGGGGCAGTAATGAAAATG10.7751937984496124No Hit
AATCAGGGTTGATTTCTTTACCTTTAGCAACCGCTAAAGCGCTAGCGAGC10.7751937984496124No Hit
TCGCGGAAATGAGCGACACCTACCGCCGCATCCGCATGCGCCTGTCTCTT10.7751937984496124No Hit
CTCCTGCACCGGACCGGCAGGGGTGCCGGAGCGCTGCAGGCCTCCGTGAA10.7751937984496124No Hit
GTATTACAGTCCGGAGAAACTGGCTGAAGATATTAGAAATACAAAAGCAA10.7751937984496124No Hit
GATAAAATATGACCTTGAACTTGGTGATATTCAACCAACTGAAGAGCCAC10.7751937984496124No Hit
CCGCAGAACAAGCCGTGGGCGCGCACGCGGTGGACTCGCCGTCCCAGGAC10.7751937984496124No Hit
CATATAATCACCTAAGAAAAAAACCCCTTGTAAAAAGGGGTTTTAAAAAT10.7751937984496124No Hit
GAAGAAGGCCTTGCGCGCATGCGGGACACCGCCGCGAAGCTGCGCTGGCT10.7751937984496124No Hit
TGCTGAAGCAAACTTACGTTTAGTTGTTTCAATCGCAAAACGTTATGTTG10.7751937984496124No Hit
GTGTCAGAATTACTGACCGCGCCCTTGTTGCGGCTGCGGAATTATCCGAC10.7751937984496124No Hit
GGATGAAGAACACAATCAGGCAGTTGTGATCAAGGAGTACGTTGAAAATA10.7751937984496124No Hit
CGCGAGGCGCCGCGTGCCGTCCACGTGCGGGTACAGCACCCAGGCCGCCG10.7751937984496124No Hit
GATTCATGATAAAGTTGTCCCTTATGACCATGCGAAGGAATTTGTTGAAG10.7751937984496124No Hit
AGCATATCAATGACGATCAGATCACAGGCAAAATCCATATCACGCAGTCG10.7751937984496124No Hit
GCATTGAGCTGGTCACCGTCGCCGCCGCAGAATCCGAGGACCCGCAGGCG10.7751937984496124No Hit
CATCATGCACGCCGCCGAAGGCAACGACGGCGTCGTGCTGGGCAAGCTTC10.7751937984496124No Hit
CCCGGGGTGCTTCCAGTGTTGGTCGGGTTCTCGGGAGTAGTCATTTCGGG10.7751937984496124No Hit
GTTTTGTGACCTGCCCCTGCGGCTTCTTGTGTGCGGGGTTTCGTGGCCCA10.7751937984496124No Hit
GATCTGATACACCACTGATTTTCTCCAATCTGAAACAGCCACTTTAAAAA10.7751937984496124No Hit
GGTGAAGACAGATAAATTCACACAAGCATTGGAAAACCAGCATAAGCATA10.7751937984496124No Hit
GAGAGTGAGATGGCGGCCATGCTGGATCCTTTCGAAGTGTCGTGTGACGG10.7751937984496124No Hit
ACATCTACGCGAGAAACGCTCCCACAGCGCTCATGCGCGACATCACAGAG10.7751937984496124No Hit
CGGCGATGCCGTCCACGCTCGCGTCCACCGCGGAGCGCACGAGGTTCGCC10.7751937984496124No Hit
CCATATTTATGGGCTGCCGGGATTTTTATCTTCAAGAGCACATCAGGGTG10.7751937984496124No Hit
GGTCAGGATAGTTGTTTGGAGTTGTTTAGAGGGAAACCCACCGAACACTC10.7751937984496124No Hit
ATGGATAAGATTGCGATGAGTTCAAATAATCAAGGCGGAGTTTCACGTGA10.7751937984496124No Hit
CTTCCAGACCCGATAGCGCCCCAGACTTTCTGCTCTCGCGTGGCGACGGC10.7751937984496124No Hit
CTGTTACACCTCGCAGCGAAGTTGTTGGAACTGATGCGGACTCGGTTCTA10.7751937984496124No Hit
CTTCCACGGCGAACGAGGCAGGCATCCCCCCGTGCTCGTACGGCACCCCG10.7751937984496124No Hit
GATTTCGACAGCTCGCCGGACGCGACGCTTCCGAGCACGTCTTTGATGTA10.7751937984496124No Hit
CCGGAGGAGTTCCACGTCCTGCGGGGAGCAGGCACGGAACCCCCAGGCGT10.7751937984496124No Hit
GACGACTGCGCCTCAGCGACGGGGACTTGAACAGTGCCGACGGCGAGCGC10.7751937984496124No Hit
GTGCTGAGAAATGGCCCTGCGCCCCTCGGGAGCATGTCGCTCCACTCTGG10.7751937984496124No Hit
CAATGACACAGAGCTGGATTAAGAGCGTTCTTAAAGATCTCGATCCGTCG10.7751937984496124No Hit
CGCGCACGCAACATGTGAACGTTGCCGCGAGACTCAACCAGCTCTAGGGT10.7751937984496124No Hit
GAAACTTTCCCGCAGTCTACAGCTTGGAAACTCACATTTTGGATTGTGAT10.7751937984496124No Hit
GTGAAGGCATATTTGACGCAGCGCATGAAGGCCTTGCCCGCTGGTCGCAC10.7751937984496124No Hit
GGTGACGATTGCCACCAGCCTGGCTGTGACGTTCATCTCTTCCATCGGCA10.7751937984496124No Hit
ATCATTACCGCGCACTGCGTCATGTCAGAATTCCCTTCTAGAGTTACTTC10.7751937984496124No Hit
CACCATTTGGACTAGATGGATGAGTGAATCCTTCTGGAAGTAAATCTTTT10.7751937984496124No Hit
CCTCTACGAGATGGACTACCTCAAGGAGGGCATCGGCTTGCGCGCGATGG10.7751937984496124No Hit
TGCATTGGCAGGTCAAAAAATAATTGGTTTTTCTTACCGTGTTTGTATAA10.7751937984496124No Hit
ATGCAGGGTATGACCTCGGACGTAATAACACGATTTTCACATTATCACTG10.7751937984496124No Hit
TGCTGGTGTGGGTGCCGGTTGTCGTGGCCATCCTGTCCGGCCTGGCCGGC10.7751937984496124No Hit
CCCTCCTTGTCGGTGGACGGCGGTGTTTCCCAGCTGTAGGTTGCGTCCGC10.7751937984496124No Hit
GGTCGGAATTTTTTCTTCCTTCAATGACTTTTTATTTTTTGTCAGAGGTT10.7751937984496124No Hit
TCCTCGGCCTGCAGGCCGGCCACGAGCTGCATGACCACCTTGGCGTCGAG10.7751937984496124No Hit
CGCTTCGCCAACTTCTTGCTCGCCAACGTCAGTGTTTCCGGAGCGCGCTT10.7751937984496124No Hit
GTGATGGTGTGGTTCGGCTTGCCCTTGTTCGTCTCGGTGATCTCCACCTG10.7751937984496124No Hit
GTATAAACTCTCGTTGCATCTCGTCGACATCGCAGACTTGGATGTTTCCG10.7751937984496124No Hit
ATATTTTCATTGTATTCGGTAAGCTTCTGATTCACTTCATAAAATATTGC10.7751937984496124No Hit
GTCCCTGTACCACGCACTGGTGCGCAAGGGAGCGATGCGCACGATGGTTA10.7751937984496124No Hit
GCTTAAGGGTTGCGCTCGTTGCGGGACTTAACCCAACATCTCACGACACG10.7751937984496124No Hit
ATGATAACTATTTCTTTCTCTGCCACTAAAGGCGGAGTGGGTAAAACAAC10.7751937984496124No Hit
GTGCCGTAGTGCTCTCGTAAAAAGTCTTCGTCTTCTGTTGTCCAGCTTCG10.7751937984496124No Hit
TATCAGTGCCGATACTTATTTCGACGGCCGCTAAAAAACTGCCTTCGACA10.7751937984496124No Hit
CGCCGACGGTGACGCCGACCAGGTTGCCCAGGCCCACAAACAGCACGACC10.7751937984496124No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)