FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003650352

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003650352
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences129
Sequences flagged as poor quality0
Sequence length146
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGAATCATTCACCAATGAAAGGTTGCTGATCATGGCAAACGTCAACAT32.3255813953488373No Hit
AAATTTTCCAGAACAGATAGATGTTGCTTCACGCATGTTAGACTGGCTTG32.3255813953488373No Hit
CTGCTGGACCATCTGGTCAAGGGATCCAAAGAGTCGAGGATCGCCGCAGA32.3255813953488373No Hit
CTTACATATAATGGGTAAAATTAAATTAGTTTAATGTTACGCCAGCTTGG21.550387596899225No Hit
GGTCGTCGTTAAGCGAAAGCCCCTCGACCACATGCCAGGCGCCGTCGCTG21.550387596899225No Hit
GATTGTTTCCATTTCTTATCATGATGAAAAGATACTGGATGGCACGGTGG21.550387596899225No Hit
TAGCCACCTTTGATGCAAAAAACGCCATGTGAATTTCATGTTATATTGAA21.550387596899225No Hit
CGATGAAACTGCAGGCCAACCTAATATTTCGGAACCGAATGAAGTGATTG21.550387596899225No Hit
ACATAGCCCGGCGGTGCGCCGATGAGTCTTGAAACGGCGTGTTTTTCCAT21.550387596899225No Hit
ATACACTTCGGAATATACTTCAACACCGTCACTTTCATCAATTTCTGCAT21.550387596899225No Hit
GGCACTAGGGGGCGGCATGGCAATTCAAGAGATTTTCGTTCACTCCGACT21.550387596899225No Hit
GGCTAAAGTCATTAAAGCTTTTAAACTTAAACCTGACTGTCATTGTACAT21.550387596899225No Hit
GTGCAGCAGGACCCTGTGGTCAGCCAGGTCAAGCTCATCGCCGAGCCGTG21.550387596899225No Hit
AATTGAAGGTATATTTTTGACCGTGACAGTACTGGTGTTTCATTTCATTC21.550387596899225No Hit
CCTTGCAGCAGCCTTCGTTCCCGATTCAACCGTTTCTTTATGTTCAAAAC21.550387596899225No Hit
CGTCTATATTTATCATCGAAAGTCTCTCTTTTATCATCATCATGACGCAT21.550387596899225No Hit
TCGGAGTCCCTTATCGAGGAAGAGGACCTGTCCTGGCTGGGTAAACACGT21.550387596899225No Hit
GTTTTAACGTCGTAGTGCGCAGTTTTATCTTCAACTTTATTTAGTTTCTG21.550387596899225No Hit
CCCAAATCCAAGAAATGTTGCCATTCGGCATTGAGGGGCCAATGGCTGCG21.550387596899225No Hit
CGATCGTTTCACTGATCGCTTTGAGTTATTTATTGCAGGTAATGAATATG21.550387596899225No Hit
AAATACTATTTTAAATGCAAAAACTTTAAATGGAAAAGAGTTATCATATA21.550387596899225No Hit
GGCCACCACCGGGGTCACGCCAGCCGCGGCCAGCTGGGCCAGCTCCGCGT21.550387596899225No Hit
CATAGATTCTGTTACCAATAGATTTTCTAAATTCATCTGGAACAAAAGTT21.550387596899225No Hit
CGCGGAGATGGTGCCGGTCAGTTCCTTCATGATCTGCGGCACGGCGATGC21.550387596899225No Hit
GACCTGGCCGTTGACGGTGAGGATGACCTGGCGCATGCCCTTTTCGTCCG21.550387596899225No Hit
GCTACGCGCGCCAGCTGGAGCGCGGCGGCGATGACGGCGTGATCGCCCTG10.7751937984496124No Hit
GTATGGTAGGATTTTTGTCCTTTTGGTGACTTCGGCGCAAAGATATGTGT10.7751937984496124No Hit
GTGATACAATCTGCTTTTTTGATATAGGTTCCAGTCTGATGAACCTTGAG10.7751937984496124No Hit
TCCGAAATCCGGCTCATGCGCCGCCGCGACCGCGAGGACTTTTAACCCGC10.7751937984496124No Hit
TATCCGACGAACATCTCGTCCCCCGAGGGATTCATTCTCAAGCTGCACCG10.7751937984496124No Hit
CGGTGAGGATCTGCACCCGGTCCTCCTTCACCGCCACCGAGCCCGGGGCC10.7751937984496124No Hit
GACGGCACCAGGCGGGCTTACGCCGCGAAAATCCGCGACGGCAAGCTGGT10.7751937984496124No Hit
GTGCCCGGAGTCATTTCGATTACAGAAGGATTCTGGCTGTTGTTCTCAAT10.7751937984496124No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10.7751937984496124No Hit
GTCTACCGTCTGATCGGGAGGATCTGCGGCACCACCACCGGACGTCCAAT10.7751937984496124No Hit
AGCACGTCGCGTGCGTCCAACATGAACCGGTCGTCCTCGCTCAGATGAGT10.7751937984496124No Hit
AACTATGATGATATCCAGTCAGTCATCATCCGCGATATTGTAAAAGAAAG10.7751937984496124No Hit
ATATGACGGTTTGTCAATTTAAGTGCAACAGTTCTTCCTGAAACGCTTCG10.7751937984496124No Hit
GATGAGAAGAACGTGGCCAGGGTGCCGTCGATAGGCATGGTGCTCCCCCT10.7751937984496124No Hit
GTCGTGGTCTACCAGCGAAACGTCCTCACCAACGAGGCTCTTCACAACGC10.7751937984496124No Hit
GTCCATCACCATGCGCATCGCTTGAACATACAGAAGAACTGCAGTTGGAC10.7751937984496124No Hit
GTAGAACATCGCGATTGTACCGTTGCCTGTGTGGCTTGAAATTGTCGGGC10.7751937984496124No Hit
GCGCAACATCTGCTCAAGTTGCGCCTCGTTGATACGACCCGCGGTAGCCG10.7751937984496124No Hit
TGCAATTGCGAAAAAAACGAAATTAACATCTTTAGGCACGAAATTTTGAA10.7751937984496124No Hit
CAATTCAGCAGTTCTGCCGAACACTGACGAAATCAAATACTGCCGACCGA10.7751937984496124No Hit
CCGCAACGTCTTCCTTGCCCGCCACACGGGTCGGCGCCGAGGGCTCCTCC10.7751937984496124No Hit
GCGGCGGAGTCGGCCCCGCCGATCTGGTCGAACGGCAGAAGCAGCACGAT10.7751937984496124No Hit
CCAAGTCCGGCTGGAATAACTCCCAAAAGGATCTTTTCCGCCGCGCCGCT10.7751937984496124No Hit
CGCCGAGGCTGAAGCGGCTGAACGCGAAAAGGCAGAAGCTTCTCGAACTG10.7751937984496124No Hit
CATATTGAATATGAAGGTGAGAAGTTTTATTTGATGCCGGATGCAGGCGA10.7751937984496124No Hit
GTAATTTTCTTCATTGTTTCTGGTTCTACTAAGTGATCAATATCATCTTC10.7751937984496124No Hit
GAGCCGAACTCGCCGCCGTTGAACCAGAGCATGAACACGCGCCACATGCG10.7751937984496124No Hit
GTCAATCACAGCTAACAACGAATAGTGTAAAATGACTTACGCAGCGTTTC10.7751937984496124No Hit
GCGCATGCGGATGCGGCGGTAGGTGTCGCTCATTTCCGCGACTGTCTCTT10.7751937984496124No Hit
GATCCAGCTCGGTCGGATTCGAACCCATTTCCGTACGAATCGTGGCGCTC10.7751937984496124No Hit
GAGATGGACAGCGACCCTGCATGGGCGGCACAAAATCTTGCAGGCGATCT10.7751937984496124No Hit
GGTCTAGCGACGTGATGCCGCGCGGGTAGAACGGCTCTGTAATCAGAATG10.7751937984496124No Hit
AGCCGAATCCTTCGCCACCTTCGAGATTTGGGTAAAGCGAAGATTGCCGA10.7751937984496124No Hit
ATACGCACTCTGAACAATTGTTTCTACAGTACGAGCATTTAAAGGCACTT10.7751937984496124No Hit
CCATTACCTATCTTTTATGGTGAAAATGGTGAACCAATTATGACAGAAGA10.7751937984496124No Hit
GACTGATACTCTGCCGTCTTTGTTTAAAATTTCAATCGCCGCTTCAAGCG10.7751937984496124No Hit
GTATTGACGGGAGAAATGATATATCCAATCATCTGATACACGAATTCCTC10.7751937984496124No Hit
CTATCAAACGACCCAGGTGACAAGGACCCACTCTTTGGCGCTGATGACGA10.7751937984496124No Hit
GTAGGTGGCGTCGTGGATGTAGGTGTCGCGGGGAATCGACACCTGTCTCT10.7751937984496124No Hit
GTCCTATTGTAGACACGAATTATTCTCCTGTGCTTTGCCGGCTACGGCGC10.7751937984496124No Hit
GCAATTGATGCTGCACCAATCATACCCCCTAATGCTTCTGCTGTCTCTTA10.7751937984496124No Hit
GTCATGGGGGAATGATACCAATTAATATGCTAAACCTTTCAAATTTTAAG10.7751937984496124No Hit
GCGTCGAACTTCTCGGTGGAGCGGAACAGCTCGGCGCCGCACGCGCGGCA10.7751937984496124No Hit
CTGTACAAGTTGCCACGGCGGTGAACTGGCAGGTGGGTCAGGTCCTAACT10.7751937984496124No Hit
CAAATACATCGTTGATGCAATCCATACGATCCGGGACATCAAGCAAAAGC10.7751937984496124No Hit
CTCTACGTCTTCGCGTGGATCGGCGGCGTGTTCTTCATCCTCCCGATTCT10.7751937984496124No Hit
AATCCACCAAGTAGCGTAGGTTGAGAATTTAAATCCTTTTTCATAATCAA10.7751937984496124No Hit
GTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGC10.7751937984496124No Hit
CCGCAGCAGCTAACGCTCTTAATAACGAAAAGAGCGAGACTGATTCGATT10.7751937984496124No Hit
CACATACACCGTCTGCGTGCCAGGGTTCCACTTCACCTGTGCAATGTGGC10.7751937984496124No Hit
CGGGTGAGGTGGACGGTAAGGACTACTTCTTTGTCACCCCCGAACACTTC10.7751937984496124No Hit
GTCGATCTGTGTACTGGTCACTTCATTATATTCCGGTACGAACAGGTTCA10.7751937984496124No Hit
TACTGAAACACCTGTATCCTGGCACATCGGCACACGGTTGGACGGTGCAA10.7751937984496124No Hit
CTTATGAAGCCGTGAAGAAACTGCCGGATAAGATCGGCCGGTTTGAAATC10.7751937984496124No Hit
CCGTAAACAGCATGGACTGCCAGTTCGACGCCGTTAGGTCCTCGGCGAAC10.7751937984496124No Hit
GTCCATATATTGTAAGTGGAAGATTTTCACCACCCTGATGTGCTCTTGAA10.7751937984496124No Hit
GACCACCGGCCGTGACGGCCGGGTCAAGCCGCTCGACGCCAAGGTGGTCA10.7751937984496124No Hit
GAAAACAGCGGCGGCACGGCGGGCGGCGTGGACACCCCGCGATACGTGGT10.7751937984496124No Hit
GTCCTGGGACGGCGAGTCCACCGCGTGCGCGCCCACGGCTTGTTCTGCGG10.7751937984496124No Hit
GCACCTGGTTATCGACGTCCTGCTCGCGCTCGCCGTCGTCGCCGCCTTTA10.7751937984496124No Hit
AATTATATGATAAGGTTGTTTTACCCACTCCGCCTTTAGTGGCAGAGAAA10.7751937984496124No Hit
GCTCTCAGGAGACGGTCTTCGCCAAAGTCACCGTCACACCGACCCCGCAG10.7751937984496124No Hit
CTCGGAAGCTGGAAAATATACACAAGCTCGTCGGCGAGCGTCTGATCCCA10.7751937984496124No Hit
ACCATACCTTGCTCGCTAGCGCTTTAGCGGTTGCTAAAGGTAAAGAAATC10.7751937984496124No Hit
GGTATCGAAATTGTCGGTACCATCCAAAATGATATCCGCTTCTTTTGCCA10.7751937984496124No Hit
GACCCTGGCAGCCTTCGGGCTGATCGTCAGCGCGGGCGCCAGGACCCTGA10.7751937984496124No Hit
CTACCCGATCTCACCGATGTTTTCGCCGAGCTCATGCGCATTTTCTCCCC10.7751937984496124No Hit
CTGTTCGAGACATTGCGACAACAGCCGTTGGCAGCATGTACCGAAAACAA10.7751937984496124No Hit
CTACCCCGGACGGCAAGAAGATCTCCTTCAACGCCTACGACAACTACGTA10.7751937984496124No Hit
GTGCGAGTACCCATTGAAGTATTTTTACATTCATCTTCTGGCCGGCTTTG10.7751937984496124No Hit
AGCACCAATAAATAAAATATGCTCAGTATTCACGGGTCCATATTTTGTTT10.7751937984496124No Hit
GAGATGCACAAACTCGTAAAGTCCCTTACCAAGTTGTCGTTGGTGACAAA10.7751937984496124No Hit
GCAGGGTGTGGTGGCGCAGGTCGGAGTTGAAGTGCTCCAGCTCGTCGGTG10.7751937984496124No Hit
GCATTGCGTTGAGCTTGGAACCCTCGCCGACGCGGTAGTACGGGTGCGGC10.7751937984496124No Hit
GTCGGCGCGCATCTCATCATCGAGCTTTTTGTCGTTGGTGCTGCGCTCTA10.7751937984496124No Hit
GTCAGATGATGTCAGTTTTCTTGTTGAATAGTTGACATAAAAACACGCAA10.7751937984496124No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)