FastQCFastQC Report
Wed 31 Aug 2022
EGAF00003650421

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003650421
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23
Sequences flagged as poor quality0
Sequence length146
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGGACAGAGGAGATCATCGGGGGCCATGAGGCCAAGCCCCACTCCC313.043478260869565No Hit
AATACAACTTCTGTGGGTTTTGGGCCATTGGAGTTTGCACTAATATCTTC313.043478260869565No Hit
CCCCCACCCTGAGCCTCTGAAAGCTGACTTTATCTGTAAGAGGGAGGTCA28.695652173913043No Hit
GTTTTTTGTAGAGATGGGGTTTCACCATGTTGCTCAGACTGGTCTCAAAC14.3478260869565215No Hit
GTGGCCGGGCACAGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAAGCC14.3478260869565215No Hit
CCTCAGGGTGGCCTCATGGTCAGAGACGGGATGGTGGGTCACGTGTGTCT14.3478260869565215No Hit
CTGCTAGAGGGTGTGGACCTGGTGTTTGGGTGAAAGTCATCCTCATCATC14.3478260869565215No Hit
CACTCCTGATACAGGCCTCATTTTGCCTGTGTACTCCTGTTGCAGGGAGA14.3478260869565215No Hit
AACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTC14.3478260869565215No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC14.3478260869565215Clontech SMART CDS Primer II A (96% over 26bp)
CATCAGGAGCCTCCACTTTCCCCAGTGCTGGATTATGCATCCTGCTCTCC14.3478260869565215No Hit
GCCATTGTTGATATGGTTCCTGGCAAGCCCATGTGTGTTGAGAGCTTCTC14.3478260869565215No Hit
ATGCATGGCCTGCCACTGATGAATCAAATTCTTAAGAACCTACGACCGTC14.3478260869565215No Hit
AACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATAC14.3478260869565215No Hit
GGTGGACAGCGAGGCCAGGATGGAGCCGCCGATCCACACGGAGTACTTGC14.3478260869565215No Hit
GGGTTGCCCAGGAGCATGGCTGCAAGGAGCAGGAGGGCCCAGGTAGCCAT14.3478260869565215No Hit
GCCTGAGACAGCTGTCTTGTGAGGGACTGAGATGCAGGATTTCTTCACGC14.3478260869565215No Hit
CACCCAGGGTATTCCATAGCTTGATGGTTTTATCTCGAGATCCAGAGACA14.3478260869565215No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)