FastQCFastQC Report
Wed 31 Aug 2022
EGAF00003650422

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003650422
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23
Sequences flagged as poor quality0
Sequence length146
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGATTTCACTGTGGAATCCAGCATAACTGGAACAACATCCAAGGTCTTC313.043478260869565No Hit
GTCCCAGAGATGGTCAGGCCCAGAGGAAGGTTAGTCTCATGCCTGCTGTT313.043478260869565No Hit
GGGCGGGGACGGGCGGTGGCTCGCCTCGCGGCGGACCGCCCGCCCGCTCC28.695652173913043No Hit
GCACTGATGGCCCTGGGAGGAACATCCTGAAGTTTAAGCCCCCAATGTGC28.695652173913043No Hit
AGGTAGTTTCGTGGACATCCGCAAAGACCTGTACGCCAACACAGTGCTGT14.3478260869565215No Hit
CTCCTGAACTGGGAGGTCACCGACCACAACCTGCACGAGCTGCGCGTGCT14.3478260869565215No Hit
CCCTGGGGGCCCTCCTGGGCCAGGCACGGGCAGGATTTCTCCTCATCACG14.3478260869565215No Hit
GTCCACTGGGTAGGAGAAGGAGCAGCGCACATGGACACACATGCCCTCTT14.3478260869565215No Hit
CCTGTGGACAAAGTGGCTGCCATGAGAGAGTTCCGGGTGTTGCACACTGC14.3478260869565215No Hit
GAGCAGGAGGGGCCGGAGTATTGGGACCGGGAGACACAGAAGTACAAGCG14.3478260869565215No Hit
GGATTACAGGCACCTGCCATCATGTCCGGCTAATTTCTGTATTTGTGTAG14.3478260869565215No Hit
GATCGCTACTACCCCGCAGTTCCCGGACATGATCCTCTCCGCCTCTCGAG14.3478260869565215No Hit
CTCCAGAGGCTGAGGCAGGACAATGGCGTGAACCCGGGAGGCAGAGCTTG14.3478260869565215No Hit
GGCTGCGTGCCAAGCTCGACCCCCTCCGCGGCCACTACTTCCTCTTCGAC14.3478260869565215No Hit
ACGATGCATTGTTATCATTAACCAGACTTTTACTACTAAACTTAAATGGC14.3478260869565215No Hit
GTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTATCAACGC14.3478260869565215Clontech Universal Primer Mix Long (96% over 25bp)
GCTCAGTGCACATAAAGTTGAAACAGAGATGGAGACATCCAGCCCCACCT14.3478260869565215No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)