FastQCFastQC Report
Tue 10 May 2022
EGAF00003650446

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003650446
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences444105
Sequences flagged as poor quality0
Sequence length146
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGATGCGTG28430.6401639251978699No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG25500.5741885364947479No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGA23560.5305051733261278No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG22790.5131669312437374No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT21970.49470282928586706No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC20720.4665563323988696No Hit
TATCTAGAGTCACCAAAGCCGCCGGCGCCCGCCCCCCGGCCGGGGCCGGA19440.4377343195865842No Hit
CCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAGACGTTCG18670.42039607750419383No Hit
CTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCATGGTAGGC17900.4030578354218034No Hit
GCATTTATCAGATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCC16920.3809909818623974No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT15940.3589241283029914No Hit
GTCTAAGTACGCACGGCCGGTACAGTGAAACTGCGAATGGCTCATTAAAT15130.34068519832021704No Hit
CTCCTCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCG14810.3334796951171457No Hit
GATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGG14700.33100280339108995No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA14510.32672453586426636No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC13820.3111876695826437No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA13310.2997038988527488No Hit
AGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGG13270.29880321095236484No Hit
CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTT13200.29722700712669303No Hit
CATTAAATCAGTTATGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGA13030.29339908355006133No Hit
AGGTTATCTAGAGTCACCAAAGCCGCCGGCGCCCGCCCCCCGGCCGGGGC12790.28799495614775783No Hit
GCCCGAGGTTATCTAGAGTCACCAAAGCCGCCGGCGCCCGCCCCCCGGCC11840.2666036185136398No Hit
TCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCC11840.2666036185136398No Hit
CTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGG11380.2562457076592248No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA11320.25489467580864883No Hit
GTTATGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGT11180.25174226815730516No Hit
AATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGATGCGTGCATT11110.25016606433163324No Hit
GCCCCTCTCCGGCCCCGGCCGGGGGGCGGGCGCCGGCGGCTTTGGTGACT11020.24813951655576946No Hit
ACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACG11010.2479143445806735No Hit
GGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCC10890.2452122808795217No Hit
GATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCG10610.2389074655768343No Hit
ATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAGACGTTCGAA10240.2305761024982831No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC10110.22764886682203533No Hit
GTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCG10000.22517197509597953No Hit
GGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCA9930.2235957712703077No Hit
GGGGGGATGCGTGCATTTATCAGATCAAAACCAACCCGGTCAGCCCCTCT9730.2190923317683881No Hit
CTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCAT9710.21864198781819613No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG9440.2125623444906047No Hit
GGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT9440.2125623444906047No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT9080.20445615338714943No Hit
GGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTT8980.20220443363618965No Hit
TCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGTAATTCTAG8980.20220443363618965No Hit
CCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCG8800.198151338084462No Hit
GGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAGACGTTCGAATG8750.1970254782089821No Hit
CGGCCGGTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCC8670.19522410240821428No Hit
CCCTGATTCCCCGTCACCCGTGGTCACCATGGTAGGCACGGCGACTACCA8460.1904954909311987No Hit
CTCCGGCCCCGGCCGGGGGGCGGGCGCCGGCGGCTTTGGTGACTCTAGAT8450.19027031895610272No Hit
GTTATCTAGAGTCACCAAAGCCGCCGGCGCCCGCCCCCCGGCCGGGGCCG8340.18779342723004694No Hit
TGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCGAATGGCTCATTAAA8280.18644239537947108No Hit
GGGCAGACGTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGC8190.18441584760360727No Hit
CGTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGCCCGAGGT8180.18419067562851127No Hit
ATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCA8150.18351515970322335No Hit
AAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGGCGCCG7900.17788586032582385No Hit
CACCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAGACGTT7750.17450828069938415No Hit
GATAAATGCACGCATCCCCCCCGCGAAGGGGGTCAGCGCCCGTCGGCATG7680.1729320768737123No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT7620.17158104502313642No Hit
GCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGA7470.16820346539669673No Hit
ATCGAACCCTGATTCCCCGTCACCCGTGGTCACCATGGTAGGCACGGCGA7410.16685243354612084No Hit
GGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAG7340.16527622972044897No Hit
ACTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAG7320.16482588577025703No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG7220.16257416601929722No Hit
CTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT7150.1609979621936254No Hit
ATCTAGAGTCACCAAAGCCGCCGGCGCCCGCCCCCCGGCCGGGGCCGGAG7010.15784555454228166No Hit
AAAGTTGATAGGGCAGACGTTCGAATGGGTCGTCGCCGCCACGGGGGGCG6980.15717003861699372No Hit
CCCGTGGTCACCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGG6980.15717003861699372No Hit
GGCCGGTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCT6940.15626935071660983No Hit
GTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCC6940.15626935071660983No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTG6750.15199108318978619No Hit
ATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCGAATGGCTCA6740.1517659112146902No Hit
CAGTTATGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTG6650.1497393634388264No Hit
CCTCGAAAGAGTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCG6600.14861350356334652No Hit
TGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTGATTCCCCG6580.14816315961315454No Hit
GAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAA6570.14793798763805854No Hit
CTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAG6470.1456862678870988No Hit
CCCTCTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCATGGT6370.14343454813613898No Hit
CTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCTTCG6360.143209376161043No Hit
CTTTTTAACTGCAGCAACTTTAATATACGCTATTGGAGCTGGAATTACCG6320.14230868826065907No Hit
CGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAG6250.14073248443498723No Hit
TATCAGATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGG6230.14028214048479526No Hit
TGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAGACGTTCGAAT6150.13848076468402742No Hit
GTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTCGCTC6050.13622904493306764No Hit
ACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGT6050.13622904493306764No Hit
TGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTA5930.1335269812319159No Hit
CCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGG5920.1333018092568199No Hit
CCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTGATTCCCCGTCACCC5900.13285146530662792No Hit
ACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACC5880.13240112135643597No Hit
GGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTGATT5800.13059974555566814No Hit
CCTCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC5750.12947388568018825No Hit
TATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATG5690.1281228538296124No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT5640.12699699395413247No Hit
GCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGATGCGTGC5600.12609630605374855No Hit
CCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGT5550.12497044617826865No Hit
TTCCAATTACAGGGCCTCGAAAGAGTCCTGTATTGTTATTTTTCGTCACT5500.12384458630278876No Hit
TTTCTCAGGCTCCCTCTCCGGAATCGAACCCTGATTCCCCGTCACCCGTG5470.12316907037750083No Hit
ACCATCGAAAGTTGATAGGGCAGACGTTCGAATGGGTCGTCGCCGCCACG5360.12069217865144505No Hit
GCCTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGGAG5350.12046700667634908No Hit
CAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTCGC5310.11956631877596514No Hit
CTCCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACA5310.11956631877596514No Hit
CCCGTCACCCGTGGTCACCATGGTAGGCACGGCGACTACCATCGAAAGTT5280.1188908028506772No Hit
CGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCT5270.11866563087558123No Hit
TGATAGGGCAGACGTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGA5230.11776494297519732No Hit
GGGCCGGAGAGGGGCTGACCGGGTTGGTTTTGATCTGATAAATGCACGCA5220.11753977100010132No Hit
GAGAGGGGCTGACCGGGTTGGTTTTGATCTGATAAATGCACGCATCCCCC5220.11753977100010132No Hit
GTTGATAGGGCAGACGTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGC5130.11551322322423752No Hit
GTTTTGATCTGATAAATGCACGCATCCCCCCCGCGAAGGGGGTCAGCGCC5110.11506287927404554No Hit
CCACGGGGGGCGTGCGATCGGCCCGAGGTTATCTAGAGTCACCAAAGCCG5020.11303633149818175No Hit
ACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCTTC4980.11213564359779782No Hit
GCCGGGGGGCGGGCGCCGGCGGCTTTGGTGACTCTAGATAACCTCGGGCC4960.11168529964760586No Hit
GTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTT4940.1112349556974139No Hit
CACGGGGGGCGTGCGATCGGCCCGAGGTTATCTAGAGTCACCAAAGCCGC4890.11010909582193401No Hit
GATTCCCCGTCACCCGTGGTCACCATGGTAGGCACGGCGACTACCATCGA4890.11010909582193401No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA4870.10965875187174204No Hit
ATCGAAAGTTGATAGGGCAGACGTTCGAATGGGTCGTCGCCGCCACGGGG4840.10898323594645411No Hit
GTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAGGAGCGAGCG4820.10853289199626215No Hit
TGCGTGCATTTATCAGATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCC4810.10830772002116618No Hit
TTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACC4780.10763220409587823No Hit
CCCCCCGGCCGGGGCCGGAGAGGGGCTGACCGGGTTGGTTTTGATCTGAT4750.10695668817059029No Hit
CACCAAAGCCGCCGGCGCCCGCCCCCCGGCCGGGGCCGGAGAGGGGCTGA4660.10493014039472646No Hit
GTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTGATTCCCC4610.10380428051924658No Hit
GGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGG4590.10335393656905462No Hit
GGGCGGGGACGGGCGGTGGCTCGCCTCGCGGCGGACCGCCCGCCCGCTCC4570.10290359261886266No Hit
AAAGAGTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGT4550.10245324866867069No Hit
TCGCGGGGGGGATGCGTGCATTTATCAGATCAAAACCAACCCGGTCAGCC4540.10222807669357471No Hit
GCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCG4540.10222807669357471No Hit
GTGGTCACCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAG4510.10155256076828678No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA4480.10087704484299884No Hit
GGGGATGCGTGCATTTATCAGATCAAAACCAACCCGGTCAGCCCCTCTCC4460.10042670089280689No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)